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initiation
where the ribosomal components, messenger RNA (mRNA), and the transfer RNA (tRNA) come together into one ribosomal unit. begins at the start codon.
elongation
the ribosome travels to the right of the start codon (towards the 3’ end) along the mRNA, and adds on to the polypeptide multiple times
termination
when a stop codon is encountered, the ribosome dissociates and releases the polypeptide along with its other translational components to recycle them
inducible gene with activator protein
no transcription because there is no inducer molecule
inducible gene with activator protein and inducer molecule
yes transcription can occur because there is an inducer molecule
repressible gene with repressor protein
yes transcription can occur because there is no corepressor
repressor gene with repressor protein and corepressor molecule
no transcription because there is a corepressor that allows the repressor to bind to the DNA
repressible gene with activator protein
yes transcription can occur because there is no inhibitor
repressible gene with activator protein and inhibitor molecule
no transcription because the inhibitor stops the activator from binding to the DNA
lactose and glucose absent, high levels of cAMP
no transcription
lactose and glucose present, low levels of cAMP
Little to no transcription
glucose present, lactose absent, low levels of cAMP
little to no transcription
what levels of regulation do eukaryotes share with bacteria?
transcription, translation, and post-translation
what level of regulation do eukaryotes have that bacteria lack?
RNA modification
which motifs function in DNA-binding?
helix-turn-helix, helix-loop-helix, zinc finger, and leucine zipper
which motif creates a dimer of two transcription factors?
leucine zipper and helix-loop-helix
glucocorticoids
steroid hormones that can cross the plasma membrane and enter cells
3 ways that regulatory transcription factors can be controlled
the binding of a small effector molecule, protein-protein interactions, and covalent modifications
what way is the action of the glucocorticoid hormone an example of?
the binding of a small effector molecule
is the glucocorticoid receptor an activator or repressor protein?
activator
what bases are purines, and which are pyrimidines?
A/G are purines and T/C are pyrimidines
is the glucocorticoid response element (GRE) an enhancer or silencer?
enhancer
histone acetylation leads to…
transcriptional activation
biological control
biological control is the use of living organisms or their products to alleviate plant diseases or damage from environmental conditions
bioremediation
bioremediation refers to the use of living organisms or their products to decrease pollutants in the environment
transgenic organism
carries a gene from another species
CRISPR-Cas technology
can inactivate a gene by introducing a deletion in it