Hgb compounds and O2 affinity

Composition of Cyanmethhgb

Ferrous iron of Hgb and Ferricyanide

The most stable among Hgb pigments

Cyanmethgb

Cyanmethgb is the reference for

Hgb determination

Absorption wavelength for cyanmethb

540 nm

Hgb that have undergone non enzymatic irreversible attachment of glucose

Glycosylated Hemoglobins

Glycosylated hbs are also called

Fast hemoglobins

In Glycosylated hbs the glucose is attached at what terminal of what chains

Valine/lysine of the B chains

Glycosylated hbs is important in testing what hb

Hb1a, Hb1b, Hb1c

Hb1c is elevated____ fold in patients with _______

2-3 folds, diabetes mellitus

Condition that is common in Px with DM

Chronic hyperglycemia

A long term indicator of blood glucose control

Hb1c

Hb1c reflects glucose for how long

3-4 months

Conditions that reflects increased Hb

Polycythemia

Dehydrations ( burns, diarrhea )

Condition where there is an absolute increase in hgb level because of true increase in red cell mass

Polycythemia

Condition where there is an increase in hgb because of decrease plasma volume leading to hemoconcentration

Dehydration

Conditions that reflects decreased Hb

All anemia

leukemia

Refers to how strong Hgb can bind to O2 at given partial pressure

Oxygen affinity

Factors affecting hb affinity for 02

Blood ( Body ) temperature

Blood pH

Level of 2,3-DPG

Carbon dioxide

Fetal Hb

Abnormal Hb

Hb has less attraction or affinity for 0₂

Right Shift

Hgb has more affinity / attraction for

Hgb has more attraction for O₂

Left shift

Alterations in blood pH, shifts O2 dissociation curve. results in an enhanced capacity to release O2 where it is needed

Bohr effect

Uptake of oxygen will release carbon dioxide and losing oxygen will increase affinity for carbon dioxide

Haldane effect