DNA Electrophoresis

What charge is DNA

Negative

DNA and polyacrylamide gels

Can separate DNA molecules that differ in length by only one nucleotide (DNA sequencing)

DNA and agarose gels

Used to separate longer DNA fragments that have larger size differences

(blue) Native-PAGE used when you want to look at

proteins and protein complexes that are still fully assembled

(blue) Native-PAGE - you can separate

proteins of approximately the same size but different charge

SDS-PAGE used when you want to look at

denatured proteins - net charged masked

SDS-PAGE - you can separate

proteins with different mass, by masking their net charge

Hemoglobin - what charge

negative at an alkaline pH

Hemoglobin migrates toward the ___ in gel electrophoresis

anode