Purification of Biotech Products and Chromatography

Flashcards focused on key concepts from the lecture on purification of biotech products and chromatography.

Chromatography

A technique used to separate, identify, and determinate mixtures of chemical compounds or biomolecules in solution based on differences in affinity for a mobile and stationary phase.

Mikhail Tswett

Russian botanist who invented liquid-adsorption column chromatography in 1903.

Adsorption Isotherm

Describes how molecules interact with a surface at constant temperature, providing insight into how much of a substance can be adsorbed under different concentrations.

Freundlich Isotherm

An empirical model used to describe adsorption on heterogeneous surfaces, indicating that as concentration increases, adsorption increases at a decreasing rate.

Langmuir Isotherm

Assumes monolayer adsorption on a homogeneous surface with finite identical sites, indicating a maximum adsorption capacity reached as concentration increases.

Linear Isotherm

A model representing the simplest case where adsorption is directly proportional to the concentration of the adsorbate.

Ion Exchange Chromatography

A separation technique that uses charged beads to attract or repel ions, allowing for the separation of ionic materials.

Affinity Chromatography

A technique used to separate and purify proteins or biomolecules based on specific binding properties to a ligand immobilized on a solid support.

Normal Phase Chromatography

Uses a polar stationary phase and a nonpolar mobile phase for separation of compounds based on polarity.

Reversed Phase Chromatography

Uses a nonpolar stationary phase and a polar mobile phase for separating compounds, commonly used in liquid chromatography.