Media Lab Blood Bank Questions

Storage lesion, or the biochemical changes in red blood cells during storage, will result in:

Decreased hemoglobin

Increased plasma sodium

Decreased plasma potassium

Decreased plasma pH

Decreased plasma pH

In blood banking, the DAT is used in the investigation of which of the following clinical conditions?

1 HDFN
2 IgA deficiency
3 HTR
4 Zika virus infections

1 3

Which of the following antigen groups is closely related to the ABO antigens on the RBC membrane?

Rh
Kell
I,i
Duffy

I, i

Which of the following statements regarding the ABO phenotype A2 is true?

A2 cells contain more antigens per RBC than A1 cells

A2 antigens are linear while A1 antigens are branched

A2 RBCs will agglutinate with anti-A1 lectin (Dolichos biflorus)

A2 cells will not agglutinate with anti-A antisera used for ABO blood typing

A2 antigens are linear while A1 antigens are branched

What is the Indirect Antiglobulin Test (IAT) usually used to detect?

Antibodies coating red cells

Antibodies in the plasma

Antigens coating red cells

Antigens in the plasma

Antigens in the plasma

The lectin Dolichus biflorus agglutinated which types of cells?

B

A1B

A2

Both A1B and A2

A1B

A D positive mother with a D negative fetus eliminates the possibility of HDFN due to the:

D antigen
ABO antigen system
Lewis antigen system
Rh antigen system

D antigen

Which of the following D variants has the best likelihood to recieve D positive RBCs without any adverse effects?

Del

Partial D

Partial weak D

C in Trans to RHD

C in Trans to RHD

What is Weak D testing not performed on pregnant women?

Weak D should be performed

Weak D is not required as part of a prenatal evaluation, as it’s not possible to differentiate weak D from partial D serologically

Weak D testing is no longer used in blood banking

Weak D testing is not reliable

Weak D is not required as part of a prenatal evaluation, as it’s not possible to differentiate weak D from partial D serologically

In the Coombs phase of a crossmatch, what is the proper procedure to follow if the Check cells give a negative reaction?

Repeat procedure with new AHG reagent and check the cell washer

Add additional Check cells and dilute with 100 % distilled water

Add additional AHG reagent plus proteolytic enzymes to enhance the reaction

Accept the crossmatch results as correct

Repeat procedure with new AHG reagent and check the cell washer

Which best describes an A1 individual?

A antigens on RBCs and anti-B antibodies in serum, which may also contain anti-A1 antibodies

A, A1 and B antigens on RBCs and no antibodies in serum

B antigens on RBCs and anti A antibodies in serum

A and A1 antigens on RBCs and anti B in serum

A and A1 antigens on RBCs and anti B in serum

for granulocyte concentrations, all of the following lab tests must be performed on the unit by the donor center except?
ABO and Rh
Red Cell antibodies
Infectious disease markers
Lymphocyte enumeration

Lymphocyte enumeration

What is the third antibody that has not been excluded? (Anti E and K are the other two antibodies?

Anti c
anti Jka
anti Jkb
anti C

anti Jkb

What kind of immunoglobulins are predominantly found in Rh immune globulin?

IgM anti-D

IgG anti-D

IgM anti-A,B

IgG anti-AB

IgG anti-D

Before testing, all cord cells should be thoroughly washed in order to:

Remove Lea substances

Remove H substances

Remove Wharton's jelly

Hemolyze contaminating maternal cells

Remove Wharton's jelly

A sample has reactions occurring at immediate spin and AHG in a panel that show varying reaction strengths. There is no obvious pattern that matches a particular panel cell or single antigen profile and the auto-control was negative. Which of the following is the most likely cause?

An IgM and an IgG antibody

An IgG antibody only

An IgM antibody only

An autoantibody

An IgM and an IgG antibody

Which of the following blood smear observations would support the diagnosis of Multiple Myeloma if a patient demonstrated plasma cells in his bone marrow and had an elevated serum IgG?

Anisocytosis

Target cells

Microcytic RBC's

Rouleaux formation

Rouleaux formation

Which of the following is the most common reagent source for anti-Al?

Group A1 plasma

Dolichos biflorous seed extracts (lectins)

Bandeiraea simp[icifolia seed extracts (lectins)

Ulex europaeus seed extracts (lectins)

Dolichos biflorous seed extracts (lectins)

Delayed hemolytic transfusion reactions are usually caused by antibodies directed against what blood group system?

MNS

Kidd

Kell

ABO

Kidd

At many hospitals, patients with sickle cell disease are given phenotypically matched units of blood. This policy is often used to help prevent alloimmunization to common RBC antigens in patients who are regularly transfused. A patient who regularly comes to your hospital demonstrates the following phenotype on her RBCs: C antigen positive; E antigen-negative: K antigen positive. The patient's doctor requests a single unit of crossmatched packed RBCs. Based on the antigen prevalence indicated below, how many units of ABO compatible packed RBCs will you phenotype to find one to transfuse to this patient?

Antigen_freguencies:

C antigen positive: 68%

E antigen positive: 22%

K antigen positive: 9%

7 units

77 units

4 units

1 unit

1 unit

78% are neg for E antigen=.78 reciprocal: 1/.78=1.3=1 unit

or 100/78 =x x=1.28= 1 unit

Which of the following represents the approximate percentage of the Caucasian population that is Rh-positive?

35%

65%

85%

95%

85%

In the interest of safety, it is the policy at XYZ hospital to always add one extra 300 pg vial of RhlG, regardless if the dosage calculation is rounded up or rounded down.

After performing a Kleihauer-Betke test, a technologist in the laboratory at XYZ hospital calculates the fetomaternal hemorrhage to be 45 mL of fetal whole blood. How many 300 pg vials of RhlG should be administered to this woman?

One

Two

Three

Four

Three

(45/30=1.5=2 +1=3)

Which of the following statements is correct regarding blood bank adverse event reporting to the FDA?

All patient deaths while being transfused must be reported to the FDA, even when it has been confirmed that the death was not related to the transfusion.

When a transfusion reaction is the result of an error it must be reported to the FDA in writing.

A transfusion-related death must be reported to the FDA within 24 hours of the patient's death.

The initial notification to the FDA of a transfusion-related death must be made by fax, telephone, express mail, or electronically as soon as possible after the death is confirmed to be associated with the transfusion.

The initial notification to the FDA of a transfusion-related death must be made by fax, telephone, express mail, or electronically as soon as possible after the death is confirmed to be associated with the transfusion.

A spectrophotometric scan of amniotic fluid may be valuable in the determination of which of the following conditions:

Neural tube defects

Maternal hypertension

Hemolytic disease of the newborn

Maternal diabetes

Hemolytic disease of the newborn