Lesson 4.2. Isolation of Polysaccharides

skin

Collection and Preparation:

1. Peel off the __________ of the root crops and banana.

2. Cut the plant material into ____________ and place in a blender or food processor.

3. Transfer the _________________ in a Ziplock or plastic container and keep in the refrigerator.

1 = ?

small pieces

Collection and Preparation:

1. Peel off the __________ of the root crops and banana.

2. Cut the plant material into ____________ and place in a blender or food processor.

3. Transfer the _________________ in a Ziplock or plastic container and keep in the refrigerator.

2 = ?

homogenized sample

Collection and Preparation:

1. Peel off the __________ of the root crops and banana.

2. Cut the plant material into ____________ and place in a blender or food processor.

3. Transfer the _________________ in a Ziplock or plastic container and keep in the refrigerator.

3 = ?

50 g of homogenized sample

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

1 = ?

100 mL distilled water or qs add

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

2 = ?

5 minutes

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

3 = ?

cheesecloth

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

4 = ?

starch

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

5.a. = ?

cellulose

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

5.b. = ?

starch

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

6 = ?

white powder

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

7 = ?

supernatant liquid

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

8 = ?

cold-distilled water

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

9 = ?

settle again

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

10 = ?

50-mL Distilled water

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

11 = ?

ethanol

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

12 = ?

purified starch

Isolation of Starch from the Homogenized Plant Samples:

1. Transfer _______________________ in a beaker.

2. Add ________________________________ to cover the plant materials.

3. Mix for ____________.

4. Filter the slurry or mixture in a cheesecloth

5. Separate the liquid (a.________) from the residue (b.__________)

6. Squeeze the cheesecloth to maximize the extraction of the _________

7. Allow the starch-containing liquid to settle for few minutes (__________ at the bottom of the beaker)

8. Carefully decant the ________________, leaving behind the starch sediment.

9. Add _________________ to the sediments and gently stir.

10. Allow the starch to ________________ and decant the supernatant.

11. Add ______________________ to the collected starch

12. Dilute it with ____________ dropwise and wait until the starch precipitate in the solution.

13. Collect the ____________________ by decantation or filtration.

13 = ?