Topic 9: Negative Staining

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Biology

Microbiology

BY 271L

UAB

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9 Terms

1
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Does the negative stain technique stain the bacteria?
no
2
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What does the negative stain technique stain?
background
3
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In negative staining, the bacteria appears ___ against the stained background.
clear
4
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Why does negative staining technique not stain the bacteria?
because of ionic replusion
5
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What is ionic replusion?
replusion of like charges
6
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What charge does the bacteria and the acidic stain have?
negative
7
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Is heat fixing or strong chemicals used during negative staining?
no
8
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Why is heat fixing or strong chemical not used during negative staining?
bacteria is less disorted
9
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Why can negative staining be useful?
in situations where other staining techniques don't clearly indicate cell morphology or size