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Topic 9: Negative Staining
The negative stain technique does not stain the bacteria but stains the background.
The bacteria will appear clear against a stained backgroun.
The stain does not stain the bacteria because of ionic repulsion (the bacteria and the acidic stain both have negative charges)
No heat fixing or strong chemicals are used so the bacteria are less distored than other staining procedures.
The negative stain technique is useful in situations where other staining techniques don’t clearly indicate cell morphology or size.
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