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This lab focuses on two sperm parameters:
motility and morphology
System used to track individual sperm and determine motility
Computer Assisted Semen Analysis (CASA)
It is important for sperm to be maintained at…
normal body temperature
Motility naturally reduces at ________temperatures?
lower
What examines how sperm have been correctly formed during spermatogenesis?
Morphology
Sperm morphology can be assessed using smear technique. Sperm are mixed with two stains:
EOSIN - stains dead sperm pink
NIGROSIN - improves contrast
Tool used to calculate number of sperm?
Hemocytometer