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gene
a segment of DNA that contains the information required to produce a product that functions in the organism
alleles
different versions of agene that have slight variations in the DNA sequence found in other alleles of the same gene
transcription
the process that creates an RNA molecule from information stored in agene
translation
the process that uses the information in messenger RNA (mRNA) to synthesize a protein
chromosome
a complex of DNA-binding proteins and DNA. they aer circular in most bacteria and archaea and linear in eukaryotes
haploid number
the number of different types of chromosomes present in a cell. it’s indicated with an n. humans have n=23
ploidy
the number of each type of chromosome present in a call. its written as a number before the n. humans are 2n
haploid
having one of each type of chromosome
diploid
having two of each type of chromosome
homologous chromosomes (homologs)
different versions of the same chromosome type
replicated chromosome
a chromosome comprised of two identical DNA molecules joined together
sister chromatids
the identical copies in a replicatd chromosome
Which enzymes are needed more frequently on the lagging strand compared to the leading strand?
ligase, primase
exonuclease
cut internally within a strand
endonuclease
cut nucleotides from the ends
what do crossover events do?
create genetic diversity
how do immune cells build antibodies?
they used information stored in genes, which is then used to make temporary information in RNA
mitosis prophase
cell prepares for division by condensing its genetic material into visible chromosomes
mitosis metaphase
where chromosomes, which have been duplicated and are visible, line up along the center of the cell at the metaphase plate
mitosis anaphase
sister chromatids separate and are pulled to opposite ends of the cell by the spindle fibers
mitosis telephase
during which the cell's separated chromosomes arrive at opposite poles, and two new nuclei reform around them
ploidy
chromosome
a DNA molecule — circular or linear, depending on the species
ribosome
a molecular machine consisting of RNA and proteins that is the site of protein synthesis
cytoskeleton functions
structural support, transport of materials, and movement in some species
flagellum
a long, flexible structure that extends from the cell and whips or rotates to propel the cell through water
which one has a nucleus, eukaryotes or prokaryotes?
eukaryotes
what do mitochondrion do?
“burns” sugars to provide energy for the cell
what are the lineages of prokaryotes?
archaea and bacteria
vacuole
an organelle that stores key molecules; very prominment in most plant cells
chloroplast
an organelle that contains the molecular machinery required for photosynthesis
endoplasmic reticulum (ER)
eukaryotic organelle that forms a branching network of narrow tubes and narrow sac. the place where proteins, membrane lipids, and other molecules are synthesized and/or processed.
what makes a rough and smooth er?
if it’s rough it has ribosomes attached, if smooth there are no ribosomes
golgi
organelle comprised of stacked, flattened sacs, where proteins and other molecules are processed and packaged for shipment to destinations
actin filaments
small-diameter cytoskeletal fibers made of subunits of the protein actin
intermediate filaments
cytokskeletal fibers whose size is intermediate between those of actin filaments and microtubules and can be made from a variety of protein subunits
microtubules
large diameter, hollow cytoskeletal elements made of pairs of the proteins alpha tubulin and beta tubulin
endomembrane system
collection of machines, cytoskeletal components, and organelles that together produce, process, and transport proteins and lipids destined for organelles, the cell membrane, or outside the cell
signal sequence
a series of amino acidsat the start of the protein that allows that protein to enter the endomembrane system
motor protein
a protein that functions in cell movement through shape changes caused by phosphorylation or dephosphorylation
phosphorylation
the addition of a phosphate group (PO43-) to a protein or other molecule. In most cases, the phosphate group comes from ATP. Dephosphorylation is the reverse process
what does it mean for the energy if a reaction is spontaneous?
the reactants have higher free energy than the products
what does it mean for the energy if a reaction is nonspontaneous?
the reactants have lower free energy than the products
electrical energy
energy related to interactions among charged particles
thermal energy
the energy of motion in ions and molecules, measured as temperature
potential energy
energy that is related to an object’s position
chemical reaction
conversion of substances into other substances via breaking and forming chemical bonds
free energy
the total energy available to do work — a combination of entropy and thermal and potential energy
energetic coupling
a phosphorylation reaction that makes a nonspontaneous reaction spontaneous, because it raises the free energy of the reactants
which cytoskeletal elements have the most diversity in terms of protein composition?
intermediate filaments
how are microtubules similar to actin filaments?
both are dynamic, with the ability to grow or shrink
which organelles contain DNA that is structurally similar to bacterial DNA?
mitochondria and chloroplast
endergonic reaction
one that results in an increase in free energy; another way of referring to a nonspontaneous reaction
exergonic reaction
one that results in a decrease in free energy other way of referring to a spontaneous reaction
activation energy
the amount of energy required to get a chemical reaction through its transition state
transition state
during a chemical reaction, an intermediate state where old bonds are being broken but new bonds have not yet formed
enzyme
a protein that catalyzes a chemical reaction
active site
the place on a enzyme where a reaction is catalyzed
redox (reduction-oxidation) reactions
chemical reactions involving the gain (reduction) or loss (oxidation) of an electron
cellular respiration
a multi-step process that uses energy captured from carbon oxidation to power ATP production via an electron transport chain and ATP synthase
electron transport chain (ETC)
a series of machines that uses an electric current to pump protons across a membrane, establishing a proton gradient that is then used to generate ATP. When the ETC uses ocygen as an electron acceptor, ATP production via the combined action of the ETC and ATP synthase is called oxidative phosphorylation
ATP synthase
the multi-protein machine that transforms the kinetic energy in a flow of protons to a mechanical energy that catalyzes the addition of a phosphate group to ADP to form ATP
glycolysis
a sequence of ten enzyme-catalyzed reactions that begins with glucose and ends with pyruvate, producing 2 ATP and 2 NADH per molecule of glucose
pyruvate processing
a serires of enzyme-catalyzed reactions that begins with acetyl-CoA, completes the oxidation of glucose to CO2, and produces ATP, NADH, and FADH2
NADH2, FADH2, Q
molecules that function as electron carriers during cellular respiration, meaning that they transport electrons to or within the electron transport chain
photosynthesis
a process that transforms light energy into chemical energy — meaning the potential energy found in electrons that participate in covalent bonds, usually in sugars or other carbohydrates
pigment
a molecule that absorbs specific wavelengths of light
chlorophyll
the primary photosynthetic pigment in land plants and most algae
photosystem II (psII)
a complex of molecular machines that acquire electerons by oxidizing water, use the energy in sunlight to excite those electron carrier that feeds the electron transport chain, leading to ATP production by ATP synthase
photosystem I (psI)
a complex of molecular machines that receive low-energy electrons at the end of the electron transport chain, use the energy in sunlight to excite those electrons to a high-energy state, and pass them on to electron carriers that either feed the electron transport chain or an enzyme that catalyzes the reduction of NADP+ to NADPH
calvin cycle
a series of reactions that results in carbon from CO2 being “fixed” or reduced, and used to synthesize sugars
rubisco
the enzyme that catalyzes the reduction of CO2 and its incorporation into sugars
stomata
openings in stems and leaves that allow gas exchange via diffusion along their concentration gradients — most importantly, CO2 to enter and O2 to leave
guard cells
specialized epidermal cells that regulate the size of stomata depending on enviornmental conditions
what are the input(s) in order for photosystem II (PSII) to work in photosynthesis?
H2O
what are the input(s) in order for photosystem I (PSI) to work in photosynthesis?
NADP2
what are the input(s) in order for ATP synthase to work in photosynthesis?
ADP and inorganic phosphate (Pi)
what are the input(s) in order for calvin cycle to work in photosynthesis?
CO2, ATP, NADPH
what are the output(s) of photosystem II (PSII) in photosynthesis?
O2
what are the output(s) of photosystem I (PSI) in photosynthesis?
NADPH
what are the output(s) of ATP synthase in photosynthesis?
ATP
what are the output(s) of the calvin cycle in photosynthesis?
carbohydrates, ADP and inorganic phosphate (Pi), NADP+
what happens to atoms in CO2 molecules during photosynthesis?
the carbon atoms end up in the carbohydrate product, and the carbon atoms will gain electrons and be reduced
when C changes from being in a polar bond with O to a non-polar bond with a C or H what happens?
it becomes reduced
when O changes from being in a polar bond in H2O to a nonpolar bond in O2, what happens?
it becomes oxidized
what about enzymes and substrates?
enzymes can only use specific molecules as substrates
in cellular respiration what does glycolysis produce?
ATP and NADH
in cellular respiration what does pyruvate processing produce?
acetyl CoA, NADH, CO2
in cellular respiration what does the citric acid cycle proudce?
FADH2, NADH, CO2, ATP
in cellular respiration what does the ETC and ATP synthase produce?
water, ATP
in phospholipids when one of the legs are bent is it unsaturated or saturated?
unsaturated
during pyruvate processing, pyruvate is oxidized to what?
acetyl-coA
in the ETC what does it oxidize?
NADH back to NAD+
what structure is required for proteins to enter the endomembrane system?
signal sequence
in order for a nonspontaenous reaction to be couple with a spontaeneous reaction, does the spontaenous reaction have to have a lower or higher free energy?
higher free energy
how does an enzyme affect the activation energy of a chemical reaction?
interactions between reactants and specific R-groups in the enzyme stabilize the transition state, which reduces activation energy
which process generates the most reduced electron carriers for the electron transport chain in cellular respiration?
citric acid cycle
how many phospholipid bilayers would you expect to find in a bacterial cell?
1
how many phospholipid bilayers would you expect to find in a eukaryotic cell?
too many to count