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Flashcards covering key terms and concepts from the Biology lecture notes, including cell structure and cycling of matter in living systems.
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Biology
The study of life, described from micro to macro levels.
Levels of Biological Organization
Molecules to organelle to cell to tissue to organ to organ system to organism to population to community to ecosystem to biosphere.
Abiogenesis
The theory that organisms can arise spontaneously.
Biogenesis
Reddi disproved it. Idea that new organisms come from pre-existing organisms.
Francesco Redi (1668)
Disproved spontaneous generation with his experiment involving meat, flies, and maggots.
Pasteur (1864)
Used an S-bend flask to allow air but not microorganisms to reach broth, disproving spontaneous generation.
Controlled Variables in Pasteur's Experiment
Light, temperature, broth type, flask type.
Manipulated Variable in Pasteur's Experiment
Access to air.
Responding Variable in Pasteur's Experiment
Growth of mold.
Leeuwenhoek
Discovered the microscope (~1680)
Hooke
Observed cork cells (~1680).
Cell Theory
All organisms are made of cells; all plants and animals come from cells; the cell is the basic unit of life; new cells come from pre-existing cells.
Three tasks accomplished by a Microscope
Produced a magnified image of the specimen, separate the details in the image, and render the details visible to the human eye or camera.
Simple Microscope
Light passes through only one lens; example: magnifying glass.
Compound Microscope
Lets light pass through an object and then through two or more lenses.
Stereoscopic (Dissecting) Microscope
Gives a three-dimensional view of an object; used for dissections.
Contrast in Microscopy
Cells appear colorless when light passes through. Staining fixes this, but kills the cells.
Resolution (Resolving Power)
The ability to distinguish between two structures that are very close together.
Electron Microscope
Uses a beam of electrons instead of light to magnify the image.
Scanning Electron Microscope (SEM)
Produces a 3D image of a specimen's surface features by bouncing electrons off a surface covered with electron-dense material.
Transmission Electron Microscope (TEM)
Allows observation of molecules within cells and magnification of objects in the order of 100,000s; provides a 2D view.
Confocal Laser Scanning Microscope (CLSM)
Uses a laser beam to illuminate spots on a specimen, and a computer compiles images to generate a 3D image; used on thick specimens.
Scanning Tunneling Microscope
Can resolve the bump caused by a single iron atom.
Microscope Magnification
An increase in the apparent size of an object, calculated as the product of the magnifying powers of the objective lens and the eyepiece.
Field of View
Area that can be seen through the microscope with a given objective lens.
Cell Theory
All organisms are composed of one or more cells; cells are the smallest living units; cells come from pre-existing cells.
Prokaryote Cell
No nucleus, single loop of DNA, few organelles; bacteria.
Eukaryote Cell
Enclosed nucleus, DNA in chromosomes, many organelles; plants, animals.
Cytoplasm
A jelly-like substance that supports the organelles inside a cell.
Cell Membrane
Encloses the cell contents; phospholipid bilayer with embedded proteins.
Nucleus
Contains genetic material (DNA); directs activities of the cell.
Nucleolus
Site of ribosome assembly.
Chromosomes
DNA of eukaryotes is densely packed into these structures.
Endoplasmic Reticulum (ER)
Serves as a system of channels from the nucleus for transport.
Rough ER
Has ribosomes attached (sites of protein synthesis).
Smooth ER
Lacks ribosomes.
Golgi Apparatus
Collect, package, and distribute molecules synthesized at one location in the cell and utilized at another location.
Lysosomes
Membrane-bound vesicles containing digestive enzymes; break down food particles and destroy old, worn-out cell parts.
Ribosomes
Site of protein synthesis; assembled in the nucleolus of the cell.
Mitochondria
Site of cellular respiration; converts stored energy into usable ATP energy.
Chloroplasts
Found only in plant cells; contain chlorophyll that absorbs sunlight and converts it into glucose.
Central Vacuole
Storage facility for water and other materials; some support for the cell.
Cell Wall (Plant)
Made of cellulose; provides support to the plant cell.
Animal Cells
Use a variety of proteins to provide support, strength, and resilience.
Centriole
Found only in animal cells; located outside the nucleus and plays a role in cell division.
Open System (Cell)
Exchange matter and energy with their surroundings and perform intake of nutrients, movement, growth, response to stimuli, exchange of gases, waste removal, and reproduction.
Cell Membrane Role
Separates the intracellular environment from the extracellular environment and selectively allows materials to cross; aka plasma membrane.
Cell Membrane Structure
Double layer, phospholipid bilayer; proteins are suspended in the bilayer.
Fluid Mosaic Model
Membrane proteins are interspersed through the membrane, with their hydrophilic and hydrophobic ends placed accordingly.
Biological Barrier (Cell Membrane)
Prevents many substances from entering cells; surrounds most organelles.
Selective Filter (Cell Membrane)
Semi-permeable – only some particles can move in and out of the cell.
Three Functions of the Cell Membrane
Keeps wastes and toxins out, allows needed substances in; surrounds and packages materials in vesicles; is a selective filter.
Types of Transport Across Cell Membranes
Passive Transport, Active Transport, Endocytosis/Exocytosis
Passive Transport
High concentration to low concentration; no energy (ATP) is required; usually gases (CO2, O2, water) are involved.
Concentration Gradient
Different concentrations of dissolved particles (solutes) exist across a cell membrane.
Brownian Motion
Matter is made up of tiny particles that are in constant motion.
Kinetic Molecular Theory
Space between particles, particles make up all materials, attraction exists between particles, and particles are always moving.
Concentration Gradient
The difference in concentration between the high and low concentration areas.
Diffusion
Molecules always move randomly and from areas of high concentration to areas of low concentration.
Passive Transport - Diffusion
Small molecules move by diffusion (water, oxygen, carbon dioxide).
Osmosis
Water molecules move by diffusion across a membrane.
Hypertonic Solution
Excess of solutes exists in the extracellular fluid; water moves OUT of the cell.
Isotonic Solution
Solute concentrations across the cell membrane are in equilibrium; no net water movement.
Hypotonic Solution
There is a deficit of solutes in the extracellular fluid; water moves IN to the cell.
Honey as a Topical Treatment
Antibacterial, anti-inflammatory, and anti-fungal properties; applied to wounds, creates a hypertonic environment that kills bacteria.
Facilitated Transport (Facilitated Diffusion)
Molecules that are too large to cross the membrane by diffusion (i.e. glucose) use this type of transport.
Active transport
Work against the concentration gradient - low concentration to high concentration - energy (ATP) is required.
Importance of Active Transport
Maintains internal cell environments (i.e. cell’s electrical gradient, roots pull in minerals from soil, filtering blood in your kidneys).
Endocytosis/Exocytosis
Larger particles are involved; the cell membrane is used to create vesicles (endocytosis) or release particles from vesicles (exocytosis).
Phagocytosis
Solids are brought into the cell.
Pinocytosis
Liquids are brought into the cell.
Diffusion and Surface Area
Diffusion over long distances is very slow and inefficient; cells maximize surface area to volume ratio for quick diffusion.
Multicellular Organisms
Multicellular organisms add more cells rather than increase the cell size; cells specialize in many different types.
Levels of Organization
Cells -> Tissues -> Organs -> System -> Organism
Photosynthesis
Carbon dioxide + water -> glucose + oxygen.
Leaf - Cuticle
Waxy coating; protects leaf from water loss.
Leaf - Epidermis
Transparent to allow light to pass through to the underlying layer.
Leaf - Palisade Cells
Main cells for photosynthesis; contain lots of chloroplasts.
Leaf - Vascular Bundle (Vein)
Organizes the two transport tissues into 'highways' throughout the leaf.
Leaf - Xylem
Transports water and minerals from roots to leaves.
Leaf - Phloem
Transports sugar (made in the leaves) to the rest of the plant.
Leaf - Spongy Mesophyll
Loosely packed with many air spaces around them – this tissue helps in water and gas exchange with the environment.
Leaf - Stomata
Openings for gas exchange (CO2 and H2O in, O2 out).
Leaf - Guard Cells
Regulate the size of the stomata (opening) to control H2O loss.
Lenticels
Allow gas flow in the stem and roots of plants.
Plant Vascular System
Extend throughout the plant; made of Xylem and Phloem; transport water, minerals, and sugars throughout the plant.
Xylem
Transports water and dissolved minerals from soil to leaves; cells linked end to end to make long tubes (xylem vessels).
Plant Transport - Water Transport
Root pressure (osmosis), cohesion/adhesion of water in the xylem, and transpiration.
Root Pressure
Water uptake from soil; the soil outside the root is hypotonic to the root cell, so water enters the cell by osmosis.
Cohesion
The attraction of water molecules to other water molecules.
Adhesion
The attraction of water molecules to molecules of other substances (xylem walls).
Transpiration
Evaporation of water through the stomata and lenticels; the 'pull' moving water up into the leaves.
Turgor Pressure
Cell walls that surround the plasma membrane and prevent water from leaving the cell.
Phloem
Transport sugar (sap) from leaves to rest of the plant.
Tropism
Plant growth in response to a stimulus (tropo - turn); can be positive or negative.
Phototropism
The growth response of a plant in response to light direction.
Gravitropism
The growth of a plant in response to gravity.
F. W. Went (1926) and Auxin
Auxin is a hormone – manufactured in 1 area and transported to another location to initiate cell elongation.
Synthetic membranes
Synthetic membranes can be used for the controlled delivery of medication and filtration across membranes
Reverse Osmosis
semi-permeable membrane, contaminates are kept from passing through