Topic 8: Staining Methods

Who introduced procedures of air-drying, chemical fixation, and staining with aniline dyes?

Robert Koch

Staining bacteria enhances ___ between bacteria and the surrounding material.

contrast

What permits the observation of greater detail and resolution than wet mount procedures?

staining

What is used to transfer bacteria from growth or culture media?

inoculating loop

True or False: Inoculating loop is sterilized only after use.

False - before and after use

How is an inoculating loop sterilized?

heating/flaming using bunsen burner or electric incinerator till loop is red-hot

What type of stain is when only reagent used?

simple stains

What type of stain is when multiple reagents used?

differential stains

What stains are used to identify specific parts of the microbe?

structural stains

What morphology are the bacteria normally grown in a lab setting?

rods or cocci

Staining and microscopic examination is usually the first steps in ___.

identifying microorganisms