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Brightfield
stained bacteria. dead specimens, can’t view viruses. directs all visible light to specimen.
Darkfield
small live bacteria, can’t view viruses, specimens are bright against dark background.
DIC (Differential Interference Contrast)
enhances 3D contrast of unstained live cells. can view eukaryotic organelles. can’t view viruses
Fluorescence
fluorescent compounds are added to dead or live cells which emit light against the dark background. can’t view viruses
TEM (Transmission electron microscope)
electron beams shine at dead specimen to view their internal structures at nm scale.
SEM (Scanning Electron microscope)
used to view the 3D surface of a dead specimen.