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Which of the following describes a key difference among the 20 amino acids that are used to make proteins?
Some amino acids are hydrophobic.
Figure 1. A short segment of a double-stranded nucleic acid molecule
Which of the following statements is correct about the molecule shown in Figure 1 ?
It is DNA because of the nucleotides present.
Different polysaccharides are used by plants for energy storage and structural support. The molecular structures for two common polysaccharides are shown in Figure 1. Starch is used by plants for energy storage, and cellulose provides structural support for cell walls. The monomer used to construct both molecules is glucose.
Based the information provided, which of the following statements best describes why starch and cellulose provide different functions in plants?
The differences in the assembly and organization of the monomers of these two polymers result in different chemical properties.
Which feature of model 1 best illustrates how biological information is coded in a DNA molecule?
The linear sequence of the base pairs
Different polysaccharides are used by plants for energy storage and structural support. The molecular structures for two common polysaccharides are shown in Figure 1. Starch is used by plants for energy storage, and cellulose provides structural support for cell walls. The monomer used to construct both molecules is glucose.
Which of the following would most likely occur if cattle lost the ability to maintain a colony of microorganisms in their digestive tract?
Cattle would no longer be able to use cellulose as a primary source of glucose.
Different polysaccharides are used by plants for energy storage and structural support. The molecular structures for two common polysaccharides are shown in Figure 1. Starch is used by plants for energy storage, and cellulose provides structural support for cell walls. The monomer used to construct both molecules is glucose.
Based on Figure 1, which of the following best compares the atomic structures of starch and cellulose?
Starch and cellulose are composed of repeating glucose monomers; however, in cellulose every other glucose monomer is rotated 180 degrees.
Which of the following best describes a structural similarity between the two molecules shown in Figure 1 that is relevant to their function?
Both molecules contain nucleotides that form base pairs with other nucleotides, which allows each molecule to act as a template in the synthesis of other nucleic acid molecules.
Figure 1 represents a segment of DNA. Radiation can damage the nucleotides in a DNA molecule. To repair some types of damage, a single nucleotide can be removed from a DNA molecule and replaced with an undamaged nucleotide. Which of the four labeled bonds in Figure 1 could be broken to remove and replace the cytosine nucleotide without affecting the biological information coded in the DNA molecule?
Bonds Y and Z at the same time
A student wants to modify model 1 so that it represents an RNA double helix instead of a DNA double helix. Of the following possible changes, which would be most effective in making model 1 look more like RNA than DNA?
Changing the deoxyriboses to riboses by adding −OH groups
Phosphorous (P) is an important nutrient for plant growth. Figure 1 shows Arabidopsis thaliana plants grown under phosphorus‐sufficient (left) and phosphorus‐starved (right) conditions for six weeks.
Which of the following is the most likely reason for the difference in leaf growth?
The phosphorus-starved plant was unable to synthesize both the required nucleic acids and lipids, limiting growth.
Water molecules are polar covalent molecules. There is a partial negative charge near the oxygen atom and partial positive charges near the hydrogen atoms due to the uneven distribution of electrons between the atoms, which results in the formation of hydrogen bonds between water molecules. The polarity of water molecules contributes to many properties of water that are important for biological processes.
Which of the following models best demonstrates the arrangement of hydrogen bonds between adjacent water molecules?
H/O\H--O/H\H
Figure 1 shows three amino acids that are part of a polypeptide chain. Figure 2 shows the same section of the chain after a mutation has occurred.
How might this change affect the structure and function of the protein?
The R-group of the new amino acid, valine, has different chemical properties than the R-group of cysteine. This will cause the protein to misfold and not function properly in the cell.
As shown in the diagram, when environmental temperatures drop below freezing, a layer of ice typically forms on the surface of bodies of freshwater such as lakes and rivers.
Which of the following best describes how the structure of ice benefits the organisms that live in the water below?
The water molecules in ice are farther apart than those in liquid water, so the ice floats, maintaining the warmer, denser water at the lake bottom.
Based on the figure, which statement best describes the observed relationship between atmospheric CO2 enrichment and plant growth under ideal and stressed conditions?
The increase in atmospheric CO2 resulted in a greater increase in plant growth under stressed conditions than under ideal conditions.
A polypeptide is polymer of amino acids held together by peptide bonds. The process of dehydration synthesis creates these peptide bonds, as shown in Figure 1.
As shown in Figure 1, an amino acid must have which of the following properties in order to be incorporated into a polypeptide?
The ability to form a covalent bond with both its NH2 group and its COOH group
Which of the following is common feature of the illustrated reactions showing the linking of monomers to form macromolecules?
Monomers are joined by a covalent bond, and a water molecule is produced.
In vascular plants, water flows from root to leaf via specialized cells called xylem. Xylem cells are hollow cells stacked together like a straw. A student explains that evaporation of water from the leaf pulls water up from the roots through the xylem, as shown in Figure 1.
Which statement describes how water is pulled up through the xylem to the leaves of the plant?
As water exits the leaf, hydrogen bonding between water molecules pulls more water up from below.
Different polysaccharides are used by plants for energy storage and structural support. The molecular structures for two common polysaccharides are shown in Figure 1. Starch is used by plants for energy storage, and cellulose provides structural support for cell walls. The monomer used to construct both molecules is glucose.
Which of the following best describes the process that adds a monosaccharide to an existing polysaccharide?
A specific enzyme removes the hydrogen (H) from the monosaccharide and the hydroxide (OH) from the polysaccharide, creating a bond between the two and creating a water (H2O) molecule.
A certain type of specialized cell contains an unusually large amount of rough endoplasmic reticulum (ER).
Which of the following functions is this cell type most likely specialized to perform?
The production and secretion of proteins
A group of mutations, known as MT-ND1, have been identified in mitochondrial DNA. These mutations are associated with a number of debilitating diseases stemming from the production of nonfunctional proteins in the mitochondria.
Which of the following cellular deficiencies would most likely be related to these MT-ND1 mutations?
The cell is unable to complete reactions related to electron transport and ATP production.
A scientist is studying the various prokaryotic and eukaryotic species found floating in a sample of water taken from a marine ecosystem.
Which cellular component will be found in the widest range of organisms in the sample?
The ribosome, since all organisms need to synthesize proteins.
In an experiment, researchers provided a radiolabeled amino acid to living plant cells. After one hour, the researchers determined the amount of the radiolabeled amino acid that was in each of several subcellular compartments. The results of the experiment are represented in the table.
Which of the following conclusions about the radiolabeled amino acid is best supported by the results of the experiment?
It was mostly incorporated into proteins that regulate and manage metabolic reactions.
Researchers conducted an experiment to investigate the effects of a valinomycin treatment on skeletal muscle cells. Valinomycin is a naturally occurring substance that can be used as a drug. The results of the experiment are presented in the table.
Which of the following claims about the effects of the valinomycin treatment is best supported by the data presented in the table?
The valinomycin treatment caused a decrease in the activity of the mitochondria.
In an experiment, researchers compared the growth of two different plants, plant X and plant Y. The researchers maintained the plants under nearly identical conditions and observed that plant X grew faster than plant Y. The researchers also observed that the inner mitochondrial membranes of plant X had more folds than did those of plant Y.
Which of the following conclusions about increasing the number of folds in the inner mitochondrial membrane is best supported by the results of the experiment?
It increases the surface area available for ATP production, which results in faster cell growth.
Some cells, such as intestinal cells, exchange a lot of material with their surroundings. The surface-to-volume ratio of these cells affects the efficiency of material exchange.
Based on the data, which cell is likely to be most effective in the exchange of materials?
Cell 2
Hereditary spherocytosis (HS) is a disorder of red blood cells that causes the cells to be smaller and spherical instead of having the usual flattened, biconcave shape. The average diameter of normal red blood cells is 7.2μm, and the average diameter of red blood cells in a person with HS was found to be 6.7μm. The normal red blood cell has an average surface area of 136μm2 and an average volume of 91μm3.
Which of the following provides an accurate calculation of the surface area to volume ratio of an HS red blood cell, as well as a prediction of its effect on the efficient transferring of oxygen compared to a normal red blood cell?
The ratio is 0.89, and the cells are less efficient at transferring oxygen.
Stomata are pores on the surfaces of the leaves and stems of plants that regulate gas exchange between the plants and the atmosphere.
Researchers found that the stomata density on the leaves of a species of plant change as the concentration of CO2 in the atmosphere changes. When grown at 350 ppm CO2 the plant has an average density of 300 stomata per mm2, but when grown at 400 ppm CO2 the plant has an average density of 250 stomata per mm2.
Which of the following best describes how the ratio of the density of stomata (stomata per mm2) per CO2concentration (ppm CO2) changes as the CO2 concentration increases?
The ratio decreases from 0.86 to 0.63, because fewer stomata are needed at higher CO2 concentrations.
Testosterone is a small steroid hormone that is important in cell signaling. Which of the following indicates where testosterone enters a cell and why it is able to cross at that point?
1, because testosterone is nonpolar and can diffuse through the membrane.
The figure shows a representation of a protein embedded in a cell membrane. The numbers indicate different structural regions of the protein.
Based on the figure, which of the following statements best describes the relationship between regions 1 and 2 of the protein?
Region 1 is hydrophilic because it interacts with an aqueous environment, whereas region 2 is hydrophobic because it interacts with the interior of the membrane.
Cholesterol is a naturally occurring substance that helps regulate the fluidity of a cell's plasma membrane. A cholesterol molecule can be represented as having a polar head and a nonpolar region, as shown in the figure.
Which of the following models shows how cholesterol molecules most likely interact with the phospholipid bilayer of a cell's plasma membrane?
The model correctly shows the polar heads of the cholesterol molecules interacting with the polar heads of the phospholipids. Also, the model correctly shows the nonpolar regions of the cholesterol molecules interacting with the hydrophobic interior of the phospholipid bilayer.
Intravenous (IV) therapy is used for fluid replacement in instances of dehydration in humans and other animals. One type of IV fluid is essentially a saltwater solution. To determine the best concentration for therapy in people, a team of students is researching the effects of solutions of different salt concentrations on red blood cells. The following observations were made from three different red blood cell samples viewed under a microscope.
The team wants to extend the research project. What should the team of students do next to obtain data that are more conclusive?
Repeat the process with other salt concentrations.
Certain bacteria can use both ethyl alcohol and acetate as sources of nutrients. In an experiment where both nutrients are available to a bacterial population, the following results were obtained and graphed.
What additional procedure would best help determine whether these movements are due to active transport or to passive transport?
Use two additional treatments, one containing only ethyl alcohol and one containing only acetate. Include a substance known to block ATP use by the plasma membrane. Compare the graphs of these two treatments to the original graph.
Researchers investigate the transport of a certain protein into cells by endocytosis. In an experiment, the researchers incubate the cells in the presence of the protein and measure the amount of the protein that is absorbed into the cells over a five-minute period.
Based on their observations, what should the researchers do to further clarify how the availability of the protein outside the cells affects the rate of endocytosis of the protein?
Incubate the cells in the presence of several different concentrations of the protein.
Lysosomes digest food particles brought into a cell by endocytosis. After a vesicle containing food particles fuses with a lysosome, H+ ions are transported into the lysosome from the cytosol. This significantly lowers the pH of the lysosome relative to the cytosol and activates the enzymes that digest the particles.
Which of the following best predicts what will happen to the lysosomal enzymes if the proteins that transport H+ ions from the cytosol into the lysosome are damaged?
The lysosomal enzymes will not become active, since there will be no active transport of H+ions.
Which of the following transport mechanisms will be affected most directly by a temporary shortage of ATP molecules inside the cell?
The transport of glucose molecules against a concentration gradient
Some membrane proteins help maintain the concentrations of ions inside a cell by transporting the ions across the cell's plasma membrane. Other membrane proteins form pores in the plasma membrane through which the ions can diffuse. A model showing the influence of membrane proteins on the movement of sodium (Na+) and potassium (K+) ions across a plasma membrane is presented in Figure 1.
Based on the model presented in Figure 1, which of the following changes will most likely result from a depletion of available ATP stores inside the cell?
The Na+concentration inside the cell will increase.
A study was conducted to understand the factors controlling the rate at which molecules or ions travel across cell membranes. An artificial membrane was created that was composed of a phospholipid bilayer only. The speed at which various substances crossed this membrane was measured.
Some substances can pass through an actual cell membrane much faster than they passed through the artificial membrane in this study. Which of the following statements best explains this finding?
Actual cell membranes have a variety of proteins embedded in the membrane that are absent in the artificial membrane.
A magnesium sulfate solution taken orally can cause a net movement of water into the large intestine, which results from water molecules diffusing through aquaporins embedded in the cells of the intestinal lining.
By which of the following mechanisms do the water molecules most likely move into the large intestine?
By passive transport from an area of low osmolarity to an area of high osmolarity
Aldosterone (a steroid hormone) is a small, nonpolar, hydrophobic molecule that enters a target cell by moving across the plasma membrane, down a concentration gradient.
Based on the information presented, how does aldosterone most likely enter target cells?
By simple diffusion
A student is using dialysis bags to model the effects of changing solute concentrations on cells. The student places one dialysis bag that contains 25 mL of distilled water into each of two beakers that are filled with 200 mL of distilled water. (Figure 1). The membrane of each dialysis bag membrane contains pores that allow small solutes such as monoatomic ions to pass through but are too small for anything larger to pass. After 30 minutes, 5 mL of a concentrated solution of albumin (a medium-sized, water-soluble protein) is added to one of the two beakers. Nothing is added to the other beaker. After two more hours at room temperature, the mass of each bag is determined. There is no change in the mass of the dialysis bag in the beaker to which no albumin was added.
Which of the graphs below best represents the predicted change in mass over time of the dialysis bag in the beaker to which albumin was added?
Graph that goes from High to low
Some viral infections can lead to the rupture of the lysosome membrane. Which prediction of the effect of this disruption of cellular compartmentalization is most likely correct?
Hydrolytic enzymes will be released, which will cause cell death.
Gaucher disease is an inherited disorder in which cells of the body are unable to break down a particular type of lipid, resulting in a buildup of the lipid in some tissues and organs.
Based on the information provided, Gaucher disease results most directly from a defect in the function of which of the following organelles?
The lysosome
Researchers have proposed a model of chloroplast evolution. According to the model, chloroplasts evolved from a small prokaryotic organism that was engulfed by an ancestral eukaryote. The engulfed prokaryote then formed an endosymbiotic relationship with the eukaryotic host.
Which of the following observations best supports the model?
Chloroplasts and some prokaryotes share similar photosynthetic reactions.
Which of the following observations best supports the claim that mitochondria evolved from once-free-living prokaryotic cells by the process of endocytosis?
Mitochondria are surrounded by a double membrane.
Which of the following claims about the origin of the euglenid chloroplast is best supported by the three-membrane structure of the envelope?
It originated from the incorporation of a photosynthetic prokaryote into a eukaryotic cell by two endosymbiotic events.
Beetroot cells contain a family of dark red pigments called betalains. The selectively permeable nature of the beetroot cells keeps the internal environment of the cell separate from the external environment of the cell. Researchers are interested in determining whether the selective permeability of beetroot cells is due to the cell membrane or if it is due to the cell wall.
Exposure to cellulase is known to damage the structure of the cell wall. An experiment is set up in which beetroot cells are placed in an aqueous solution with cellulase and in one without cellulase.
Which of the following results best refutes the alternative hypothesis that selective permeability is a consequence of the cell wall?
When beetroot cells are placed in a solution with cellulase, the solution remains clear.
Two competing hypotheses exist regarding the cell membrane structure. One hypothesis states that membrane structure is static and membrane components throughout the bilayer are rigidly bound. Alternatively, the other hypothesis states that cell membranes are a fluid mosaic in which membrane components may drift within the bilayer around the surface of the cell. An experiment is set up in which membrane proteins of two different cells are fluorescently labeled with two different colors and then fused as shown in Figure 1.
Which of the following results, one hour after membrane fusion, best supports the alternative hypothesis that the cell membrane is a fluid mosaic?
Image w/ mixed up gray and black dots
The cell membrane is selectively permeable due to its structure. Thus, the internal environment of the cell is distinct from the external environment of the cell. One biologist hypothesizes that small nonpolar molecules readily pass through the membrane. Another biologist alternatively hypothesizes that these types of molecules require channel and transport proteins that are embedded in the membrane in order to move across the membrane.
Which of the following data would best refute this alternative hypothesis?
CO2 and N2 movement in and out of cells is unaffected when membrane protein activity is blocked.
Aminolevulinate dehydratase (ALAD) is an enzyme that relies on zinc as a coenzyme. A zinc ion binds to the ALADactive site, where it forms favorable interactions with the side chains of three amino acids. Researchers have found that substituting a lead ion for a zinc ion in the ALAD active site causes inhibition of ALAD.
Which of the following statements best helps explain how the lead ion causes inhibition of ALAD?
It changes the three-dimensional structure of the active site so that ALAD is no longer compatible with its substrate.
Acetylcholinesterase (AChE) is a protein that catalyzes the conversion of acetylcholine to acetate and choline. When the concentration of AChE in an aqueous solution is held constant, the rate of the reaction catalyzed by AChEincreases with increasing concentrations of substrate. At low concentrations of acetylcholine, a small increase in the substrate concentration results in a large increase in the reaction rate. At high concentrations of acetylcholine, however, a large increase in the substrate concentration results in only a small increase in the reaction rate.
Which of the following statements correctly explains the observed effect of the acetylcholine concentration on the rate of the enzyme-catalyzed reaction?
The active site of AChE is specific for acetylcholine, and only one substrate molecule can occupy the active site at a time.
A researcher proposes a model to explain how enzyme-substrate interactions determine enzyme specificity. The model is based on the idea that substrate molecules form favorable interactions with the amino acid side chains in an enzyme's active site.
Based on the model, which of the following statements best explains an enzyme's specificity for a particular substrate molecule?
A molecule with negative charges interacts with positively charged side chains in the enzyme's active site.
Catalase is an enzyme found in yeast cells that facilitates the chemical breakdown of hydrogen peroxide to water and oxygen gas. An experiment was conducted to determine the effect of pH on catalase function. Five buffer solutions of varying pH (2, 4, 6, 8, and 10) were prepared and added to separate test tubes. Hydrogen peroxide (H2O2) was added to each test tube. Yeast was added, and the reactions were timed. After 1 minute the amount of oxygen gas released was determined by measuring the foam layer produced in each test tube. Figure 1 illustrates the experimental setup.
A set of five additional test tubes were prepared and used as controls. Which of the following best describes the contents expected to be contained in one of the five control test tubes?
pHpH 4 buffer solution and hydrogen peroxide only
A student designs an experiment to investigate the influence of temperature on enzyme function. The student's plan is presented in Table 1.
Which test tubes are controls in the experiment?
Test tubes 1, 3, 5, and 7
A researcher designs an experiment to investigate whether soil bacteria trigger the synthesis of defense enzymes in plant roots. The design of the experiment is presented in Table 1. For each group in the experiment, the researcher will determine the average rate of change in the amount of defense enzymes in the roots of the seedlings.
Which of the following statements best helps justify the inclusion of group 2 as one of the controls in the experiment?
It will show whether the changes observed in group 1 depend on the metabolic activity of soil bacteria.
Pectinase is a protein that catalyzes the breakdown of pectic polysaccharides in plant cell walls. A researcher designs an experiment to investigate the effect of salinity on the ability of pectinase to lower the activation energy of the reaction involved. The design of the experiment is presented in Table 1. For each test tube, the researcher will measure the amount of product formed over 20 minutes.
Which of the following statements best helps justify the inclusion of test tube 5 in the experiment?
It will act as a control for test tube 6 by showing the effect of the presence or absence of the enzyme.