Histo Lab-Cardiovascular Sysytem (Aughey)

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Artery (dog). (1) Lumen. (2) Tunica intima. (3) Tunica media. (4) Tunica adventitia. Masson’s trichrome.×62.5.

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Artery (dog). (1) Lumen. (2) Tunica intima with the internal elastic lamina (arrowed). (3) Tunica media. Masson’s trichrome. ×125.

name of histologic slide:

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Aorta (horse). Elastic artery stained to illustrate the wavy elastic fibres. Weigert’s elastin. ×100.

name of histologic slide:

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Muscular artery (sheep). (1) Lumen. (2) Tunica intima with the internal elastic lamina. (3) Tunica media. (4) Tunica adventitia. H& E. ×125

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Muscular artery (sheep). (1) Lumen. (2) Tunica intima. (3) Tunica media. (4) Tunica adventitia. Masson’s trichrome.×250.

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Artery and vein. Stomach (dog). (1) Small artery. (2) Small vein. (3) Lymphatic vessel. H & E. ×62.5.

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Arteriole, vein and a Iymphatic vessel in connective tissue. Tongue (ox). (1) Arteriole. (2) Vein. (3) Lymphatic vessel. H & E. ×62.5.

name of histologic slide:

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Arterioles in the wall of a small artery (horse). (1) Arteriole. (2) Veins. (3) Artery. H & E. ×160.

Three types of Artery:

elastic,

muscular (distributing)

arteriole.

Structure of the arterial wall

tunica intima (inner lining layer),

tunica media (middle layer)

tunica adventitia (outer layer

tunica intima consists of

elongated flattened endothelial cells resting on loose areolar connective tissue.

tunica media of the elastic arteries has a

-high proportion of concentric lamellae of fenestrated elastic fibres interspersed with smooth muscle fibres (these allow the vessels to dilate)

-consist of only one layer of smooth muscle cells, and the luminal diameter is less than the thickness of the wall

composition of muscular (distributing) arteries

The elastic content is reduced and the smooth muscle increased.

Internal and external elastic lamina are present

arterioles

reduce the pressure of the blood and supply the capillary bed.

tunica adventitia is composed of

collagen and elastic fibres, and contains the vasa vasorum, the small nutrient arteries and veins in the walls of the larger blood vessels

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Capillaries (sheep) in the connective tissue of the cervix (arrowed). Masson’s trichrome. ×125.

capillary

capillary is the smallest unit of the vascular system.

layers of capillary wall (two-layered)

a tunica intima of one or two squamous endothe lial cells resting on a basal lamina,

a fine tunica adventitia of collagen and elastic fibres.

pericytes:

undifferentiated cells believed capable of becoming fibroblasts or muscle cells

Venules

collect the blood from the capillaries.

Their lumina are wider than those of the arterioles.

The tunica intima (there is no tunica media and adventitia) in each venule consists of a continuous layer of endothelial cells and areolar connective tissue.

Pericytes are also present.

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Liver (sheep). (1) Central vein. (2) Liver sinusoids. H & E. ×62.5.

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Spleen (horse). Sinusoids filled with erythrocytes. H & E. ×125.

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Pars distalis of the adenohypophysis (cat). (1) Sinusoids. (2) Cords of hypophyseal cells. Orange G. ×250.

Sinusoids

found in the liver, spleen, bone mar row and adenohypophysis.

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Spermatic cord (horse). Venous plexus (arrowed). H& E. ×62.5.

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Caudal vena cava (dog). (1) Tunica intima. (2) Tunica media. (3) Tunica adventitia with vasa vasorum (arrowed). Gomori’s trichrome.×125.

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Caudal vena cava (horse). (1) Tunica intima. (2) Tunica media. Masson’s trichrome. ×125.

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Cranial vena cava (horse). (1) Tunica intima. (2) Tunica media. H & E. ×125.

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Femoral vein with valves (cat). (1) Lumen. (2) Valve. (3) Tunica intima. (4) Tunica media. (5) Tunica adventitia. Masson’s trichrome. ×25

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Valve in the brachial vein (cat). (1) Lumen filled with erythrocytes. (2) Valve. (3) Tunica intima. H & E. ×125.

Veins

lined by a continuous layer of endothelial cells and areolar connective tissue

name of histologic slide (clinical correlates):

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Arteriovenous anastomosis in loose connective tissue (cat). (1) Artery. (2) Vein. H & E. ×62.5.

Arteriovenous anastomoses

are special areas of the skin of the nose, lips and pads where the arteriole opens directly into a venule without going 6.19 through the capillary bed

This provides an alternative channel of blood supply and regulation of heat loss

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Cardiac muscle (ox). (1) Cardiac muscle. (2) Connective tissue of the fibrous skeleton. (3) Atrioventricular node. Masson’s trichrome. ×125.

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Cardiac muscle (horse). (1) Cardiac muscle fibres. (2) Conducting (Purkinje) fibres. H & E. ×125.

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Heart (dog). (1) Lumen of the atrium. (2) Lumen of the pulmonary artery. (3) Valve cusps. Masson’s trichrome. ×62.5.

name of histologic slide :

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Heart (dog). (1) Valve cusps. (2) Dense connective tissue part of the fibrous skeleton of the heart. Masson’s trichrome. ×62.5.