Dilutions

What is the purpose of making dilutions?

to have the correct amount of a specific item.

decreasing the concentration of solute with an amount of solvent

List 3 examples of dilutions made for use outside of the lab:

Adding hot water to coffee grounds or tea leaves

adding water to cool aid powder

Adding ice to a beverage: concentration of the drink decreases

List 3 examples of dilutions made for use inside the lab:

Contrex

ethanol

P30 & Hematrace

Prepare a 40% ethanol solution with a final volume of 1000mL

1000/100 = 10

40 × 10 = 400mL Ethanol

60 × 10 = 600mL H2O

Prepare a 1:100 dilution to overcome a possible high dose hook effect using the ABA card p30 test, final volume of 200uL.

200/100 = 2

in 100uL = 1uL neat and 99 H2O

in 200uL = 2uL neat and 198 H2O

Why was the modification verification performed?

because of new design

What type of dilutions were made when performing ABAcard P30 test?

serial dilution

How do you convert ng/mL to ng/uL

divide by 1000