Making Insulin
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Last updated 9:29 AM on 3/31/23
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4 Terms
1
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A restriction enzyme cuts a segment of DNA, a gene of interest. It also cuts a plasmid (vector) open.
Step 1
2
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DNA Ligase glues the Human DNA segment between the sticky ends of the Bacteria DNA.
Step 2
3
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The bacteria is made to take up this recombinant DNA by a heat shock.
Step 3
4
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Copies of the engineered cells are made during binary fission in a fermenter.
Step 4