Making Insulin

A restriction enzyme cuts a segment of DNA, a gene of interest. It also cuts a plasmid (vector) open.

A restriction enzyme cuts a segment of DNA, a gene of interest. It also cuts a plasmid (vector) open.

Step 1

DNA Ligase glues the Human DNA segment between the sticky ends of the Bacteria DNA.

Step 2

The bacteria is made to take up this recombinant DNA by a heat shock.

Step 3

Copies of the engineered cells are made during binary fission in a fermenter.

Step 4