Sem 1 Biotech kelly final

Flammable

What does this picture mean?

Autoclave

An ____ is a device that uses superheated steam to sterilize equipment used in the lab

FDA

The biopharma pipeline is regulated from discovery to approval by this organization

transfer

A _____ pipette does not have any graduations and is primarily used to transfer bulk solutions

USDA

The affect on agriculture of GMO crops is monitored by the ____

SOP

A structured document listing the equipment, personal responsibilities, procedures, and reporting of outcomes associated with lab procedures is called the ____ document

Buffer

A substance that keeps the PH of a solution relatively constant is a _____

to prevent denaturation

Why is it important to normalize PH in the practice of Biotech?

a base

What can be used to neutralize an acidic solution?

Biotechnology

____ is any technical application that uses biological systems to modify and produce useful products

Phase-3

_____ __ is the step in drug development that uses a very large group (1000-3000) of healthy individuals to determine efficiency and side effects

Healthcare

_____ is the largest sector of the biotech industry and includes the development of biosimilars, pharmacogenomics, and drug development.

SOP

A ____ is a standardized list of procedures and responsibilities used in manufacturing

USDA

The ___ is the agency that oversees animal and plant health as biotechnology improves new crop variables

Pre-clinical

___-______ is the second step in drug development which includes using animal models

Genomics

____ is the study of the full complement of an organsim's DNA

Bioinformatics

____ is the study, storage, analysis, and use of large sums of data that is generated in biotechnology.

Phase-2

____-______ is the step in drug development that uses a moderately large group (100-300) of sick individuals to determine if the drug actually treats the affliction.

Agriculture

____ is a sector of the biotechnology industry that includes the development of GMO crops and the practice of pharming

Transcriptomics

The study of mRNA generated by an organism would fall under the study of ____

Health Hazard

What does this picture mean?

EPA

The effects on the environment that biopharma agencies exert both from fractory and crop management are monitored by this agency

MSDS

The____ is a document listing all of the potential hazards with chemicals used in the lab.

Toxic

What does this picture mean?

BSL-1

HB101-K12 is a strain of E. coli used in our labs. It is classified as this safety level

1/2, lethal dose

The LD50 is included in the application to transition from pre clinical to clinical trials. LD50 is classified as ____ of the ____ ____

TAE

Name a buffer we have used this year

GLP

A _____ is a set of internationally recognized set of laboratory guidelines that are used in generating new products

control

A set of experimental conditions that is unchanged describes a ____ variable

In-vivo

When experiments and procedures are performed within a living organism they are considered _____

manipulated

A single condition that is changed in an experiment describes the ____ variable

GMP

___ are similar to GLPs but focused on the production side of biotechnology and are enacted once the research has been completed

serological

A _____ pipette has graduations all the way to the tip of the pipette

OSHA

Workplace safety is overseen by this agency

Caution

What does this picture mean?

special

What does the white part mean ?

Health

What does the blue part mean?

flammable

What does the red mean?

Reactivity

What does the yellow mean?

PPE

Gloves, goggles, and gowns are all examples of what?

responding

The measurement of the manipulated variable describes the _____ variable

nanobiotechnology

The use of very small molecules and viral vectors greatly improved by biotechnology. This created a field called _____

EPA

The ____ is the agency that oversees hopw biotechnology affects the environment.

FDA

The ___ is the agency that oversees all new medical, agriculture and food biotechnology.

Pharming

____ is the practice of using crops or livestock to produce theraputics

negative

A set of conditions in an experiment designed to not produce a change is referred to as a ____ control variable.

Phase-one

____-_____ is the step in drug development that uses a small group (<100) of healthy individuals to determine dosage and safety levels

High-throughput

_______-______ type of technology used in drug discovery that allows the researcher to screen thousands of molecules in a short period of time

Discovery

____ is the first step in drug development

GMO

A crop that has had its genome modified to resist pests or herbicides is considered a _____ crop.

Bioremediation

The process of using genetically modified bacteria to clean up pollution is called the practice of ______

positive

A set of conditions in an experiment designed to produce an expected change is referred to as a _____ control variable

forensics

The _____ industry uses biotechnology to identify people through processes such as DNA fingerprinting

OSHA

____ is the agency that oversees workplace safety in the biotech lab

Plasmid

______ DNA is the ideal vector for insertion of recombinant DNA into bacteria

positive

A stain that sticks to DNA by charge is called a _____ stain

DNA fingerprinting

The process of ____ _______ uses RFLPs and STRs to identify people based on differences in their DNA

Gel electrophoresis

_____ _____ is a process used in separating DNA fragments by size using a molecular sieve and electricity.

sticky

If the restriction enzyme cuts DNA leaving uneven ends, the ends are classified as ____

STRs

DNA fingerprinting originally took advantage of _____ regions of DNA that were genetically different repeating regions of DNA in individuals.

palindromes

Restriction enzymes look through DNA for repeating sections of nucleotides called ____

negative

The overall charge of DNA is ______

thymine

The nucleotide ____ is found in DNA but not in RNA

Ligase

____ is the enzyme that ties the sticky ends of DNA together

TAE

_____ is a common buffer used in gel electrophoresis

electrolyte

A solution that is capable of transmitting charge is called an _____

purines

A & G have two rings and are classified as _____

Pyrimidines

C & T have one ring and are classified as _____

southern blotting

The original process of using RFLPs in DNA fingerprinting is called _____ ______

fluorescent

A ______ dye makes DNA run on a gel easy to read by reacting with ultraviolet light

RNA polymerase

____ ______ is the enzyme that makes RNA from DNA in the transcription process

3'-5', 5'-3'

DNA is read in the __-__ direction and copied in the __-__ direction

Restriction enzymes

____ _____ read DNA and cut it at specific regions

promoter

DNA is inserted, at the MCS, upstream of a ____ region to ensure expression

C to G, A to T

According to Chargaff's rule, __ is bound to __ (with a triple bond) and __ is bound to __ (with a double bond)

recombinant

DNA that has been altered through the insertion of foreign DNA is now classified as ____ DNA

virus

A ____ is another name for a bacteriophage

EDTA

____ is a chelating agent used to stabilize DNA that is to be studied using gel electrophoresis

DNA Polymerase

___ ______ is the enzyme that replicates DNA

RNA

____ is made by RNA polymerase as genes are copied in the process of transcription

anti-parallel

The strands of DNA are said to be ____ as they are identical running in the opposite direction

uracil

The nucleotide ____ is found in RNA but not in DNA

genome

All genetic information of an individual is contained in a person's _____

Plasmid

Ideal prokaryotic vector is a _____

cloning

If DNA is stored or replicated, it is called a _______ vector

expression

If DNA is used to produce a product, it is called an ______ vector

transformation

Process in which bacteria pick up plasmids (from the environment) is called ______

log

Bacteria in the ___ phase are most capable to pick up plasmids

CaCl2

Exposing the cell membrane to the chemical _____ makes it more competent

heat

The process of ____ shock opens up the pores to increase efficiency

ice

Placing bacteria on ____ closes the pores, trapping in the plasmids

electroporation

Eukaryotic cells are transformed/tranfected by using an electrical current through the process of ____

shuttle

Makes use of the viral vector known as a ____ plasmid

restriction, MCS

Plasmids are opened with the ____ enzyme at the _____ site

sticky

The gene of interest and plasmid are cut with the same enzyme so it can be inserted into a plasmid because it has the same ____ ends

DNA ligase

Insertion is made permanent with the enzyme ___ _____ which joins the ends together

miniprep

Purification of plasmids from a digested bacterium is known as _____

ORI

Sit that signifies the beginning of replication ____

mcs

A _____ site where the gene of interest is inserted

antibiotic resistance

An ____ ____ gene is inserted soi that the bacteria can be grown on differential media