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what colour do gram negative bacteria stain?
pink
what are the two main shapes of gram negative bacteria?
- bacilli
- cocci
what are the two main subdivisions of gram negative bacilli?
- enterobacteriaceae (coliforms)
- pseudomonadaceae (pseudomonads)
are gram negative bacilli generally antibiotic resistant or sensitive?
resistant
are gram negative bacilli generally antiseptic resistant or sensitive?
resistant
are gram negative bacilli generally resistant or sensitive to drying?
sensitive
what are some examples coliforms?
- E.coli
- enterobacter
- klebsiella (pneumoniae)
- proteus
- salmonellae
where do coliforms live?
gut
what infection type do coliforms cause?
opportunistic and primary
what intestinal opportunistic infection examples do coliforms cause?
- appendicitis
- diverticulitis
- biliary infection
what urosepsis opportunistic infections do coliforms cause?
UTIs
what hospital opportunistic infections do coliforms cause?
ventilator associated pneumonia (VAP)
what primary infections do coliforms cause?
- salmonellas
- shigellas
- E coli
what are the gram negative antibiotics used for coliforms? (5)
broad spectrum:
- amoxycillin (50% resistant)
- trimethoprim (25% resistant)
- co-amoxyclav
- cephalosporins
- gentamicin
what are some examples of pseudomonads?
- pseudomonas aeruginosa
- burkholderia cepacia
- stenotrophomonas maltophilia
- haemophilus influenzae
when can pseudomonads cause HCAIs?
- moisture
- antimicrobial use
when can pseudomonads cause primary infection?
in contaminated water
what are two types of haemophilus influenzae bacteria?
- capsulate
- non-capsulate
what infections can capsulate haemophilus influenzae cause?
primary:
- HIB
- meningitis
what infections can non-capsulate haemophilus influenzae cause?
opportunistic:
- COPD
- sinusitis
- otitis media
what is the main type of gram negative cocci?
neisseria
what type of cocci is neisseria?
diplococci
what are the two main types of neisseria?
- n. gonorrhoeae (gonococci)
- n. meningitidis meningococci
what gram are the gram negative antibiotics used for pseudomonads and resistant coliforms?
broad spectrum:
- gentamicin
- ciprofloxacin