Endocytosis and Exocytosis

Cell Transport and Endocytosis Flashcards

1. Cell Transport Brownian Movement Definition: Random movement of particles caused by their inherent kinetic energy. Occurs above freezing temperatures. Passive process (requires no energy/ATP). Responsible for constant molecular motion. Diffusion Definition: Movement of molecules or ions from an area of higher concentration to lower concentration. Passive transport Moves down the concentration gradient No energy required Facilitated Diffusion Definition: Diffusion through a selectively permeable membrane using membrane proteins. Passive transport High → Low concentration Only certain substances can pass Filtration Definition: Movement of water and dissolved substances through a membrane from higher pressure to lower pressure. Driven by pressure differences Dialysis Definition: Separation of small molecules from larger molecules. Used in dialysis machines Not a normal transport process in the body 2. Active Transport Active Transport Definition: Transport requiring cellular energy (ATP). Endocytosis ("Into the Cell") Phagocytosis Cell Eating Engulfs solid particles Example: White blood cells engulfing bacteria Pinocytosis Cell Drinking Engulfs fluids Exocytosis Definition: Movement of substances out of the cell. 3. Epithelial Tissue Characteristics Covers body surfaces Lines organs and cavities Cells are tightly packed High mitotic rate Connected to connective tissue by a basement membrane Classification by Shape Shape Name Flat Squamous Cube-shaped Cuboidal Tall/elongated Columnar Classification by Layers Layers Name One layer Simple Two or more layers Stratified Example: Simple Columnar Epithelium = One layer of elongated cells 4. Connective Tissue Components Connective tissue contains: Cells Fibers Ground substance Fibers + Ground Substance = Matrix Loose Connective Tissue Areolar Tissue Supports organs Widely spaced fibers Adipose Tissue Fat tissue Insulation Energy storage Reticular Tissue Found in lymph nodes and spleen Acts as a filtering framework Dense Connective Tissue Dense Regular Fibers run in one direction Found in: Tendons Ligaments Aponeuroses Dense Irregular Fibers run in multiple directions Found in: Dermis of skin Elastic Connective Tissue Allows stretching Found in: Walls of arteries Elastic structures 5. Cartilage Hyaline Cartilage Found in: Articular cartilage (joint surfaces) Costal cartilage (ribs) Fibrocartilage Strongest cartilage Found in: Intervertebral discs Pubic symphysis Meniscus of knee Elastic Cartilage Provides flexibility Found in: External ear 6. Skin (Integumentary System) Epidermal Cells Keratinocytes Produce keratin Waterproofing protein Melanocytes Produce melanin Protect from UV radiation Langerhans Cells Immune defense Fight infection Merkel Cells Touch receptors 7. Layers of the Epidermis Deepest → Most Superficial Stratum Basale Stratum Spinosum Stratum Granulosum Stratum Lucidum (only thick skin) Stratum Corneum Thick Skin Found on: Palms Soles Contains: Stratum Lucidum Dead Layers Stratum Lucidum Stratum Corneum Filled with keratin. 8. Skin Glands Sudoriferous Glands Sweat glands Eccrine glands Apocrine glands Sebaceous Glands Produce sebum (oil) Ceruminous Glands Produce earwax Mammary Glands Modified sweat glands Produce milk 9. Skeletal System Upper Extremity Humerus Arm bone Radius Lateral forearm bone Thumb side Ulna Medial forearm bone Forms elbow joint Carpals Wrist bones 8 bones Metacarpals Hand bones Phalanges Finger bones Thumb: 2 phalanges Other fingers: 3 phalanges Pelvis Ilium Superior portion Pubis Anterior portion Ischium Posterior/inferior portion "Sit bones" Lower Extremity Femur Thigh bone Tibia Shin bone Medial Fibula Lateral lower leg bone Tarsals Ankle bones Metatarsals Foot bones Phalanges Toe bones Big toe: 2 phalanges Other toes: 3 phalanges High-Yield Exam Questions 1. What is Brownian movement? Random movement of particles caused by kinetic energy. 2. Does diffusion require energy? No. It is passive transport. 3. Difference between diffusion and facilitated diffusion? Facilitated diffusion requires a selectively permeable membrane protein. 4. What is phagocytosis? Cell eating (engulfing solids). 5. What is pinocytosis? Cell drinking (engulfing fluids). 6. Which epithelial tissue has one layer of column-shaped cells? Simple columnar epithelium. 7. What is the matrix of connective tissue? Fibers + ground substance. 8. Which cartilage is found in intervertebral discs? Fibrocartilage. 9. Which epidermal cells produce melanin? Melanocytes. 10. What are the five epidermal layers? Basale → Spinosum → Granulosum → Lucidum → Corneum. 11. Which bone is on the thumb side of the forearm? Radius. 12. Which bone forms the shin? Tibia. 13. What are the wrist bones called? Carpals. 14. What are the ankle bones called? Tarsals. 15. What are the hand and foot bones called? Metacarpals and Metatarsals. Quick Memorization Tips Pino = Sip → Pinocytosis = cell drinking Phago = Eat → Phagocytosis = cell eating Radius = Radio to Thumb → Radius is lateral Simple = One Layer Stratified = Many Layers Melanocytes = Melanin Keratinocytes = Keratin Sebaceous = Sebum (Oil) Sudoriferous = Sweat Carpals = Wrist Tarsals = Ankle This covers the major concepts your instructor specifically reviewed for the exam

endocytosis

exocytosis and lysosome

lec 14-Endocytosis and Intracellular Vesicle Trafficking

endocytosis and vesicle trafficking

MMiPeople Francesco Redi: scientist who tested spontaneous generation with rotting meat and maggot formation with an open container, sealed container, and gauze-covered container, and refuted spontaneous generation Needham: scientist who tested spontaneous generation with boiling chicken broth, sealing the container, and waiting a few days to assess for microbial growth, and supported spontaneous generation Spallanzani: scientist who tested spontaneous generation with further testing, Needham’s experiment, and tested with an open and closed container that was later opened, and refuted spontaneous generation Louis Pasteur: scientist who used swan-neck flasks in his experiment and definitively refuted spontaneous generation Anthony van Leeuwenhoek: scientist who was the first to observe eukaryotic microbes, calling them “wee animalcule” Robert Hooke: coined the term “cell” when looking at cork Matthias Schleiden: observed cells in plant tissue Theodor Schwann: observed cells in animal tissue Rudolf Virchow and Robert Remark: observed cells dividing to make new cells (not mitosis) Hippocrates: suggested disease has natural causes Thucydides: advocated for evidence-based analysis of cause and effect and suggested immunity after observing plague survivors didn't get sick again Marcus Terentius Varro: the first to propose that things we cannot see cause disease Ignaz Semmelweis: physician who observed the spread of disease among patients in different sides of the hospital and instituted hand washing between patients to reduce the spread of disease from patient to patient via healthcare workers Joseph Lister: surgeon who observed post-surgical infection and instituted hand washing and sterilization of medical equipment with 5% phenol solution for less disease Robert Koch: scientist who developed Koch’s postulates to determine the cause of disease and had a rivalry with Louis Pasteur John Snow: questioned the London cholera outbreak and asked questions to the people who had been infected about where they had been and what they had eaten or drank, and noticed that everyone who had cholera used one of two water pumps Classification of microbes What are the 2 domains that are composed of all microbes? Bacteria and Archaea What domain of life is composed only of some microbes? Eukarya What types of microbes do not fit into the domains of life? Viruses and Prions Prions: unicellular organisms in the domains Bacteria and Archaea with no nucleus and have cell walls Viruses: acellular and domainless (not alive) Eukaryotes: unicellular OR multicellular organisms, in domain Eukarya, have a nucleus, have membrane-bound organelles, and include fungi, algae, protazoa, and helminths How many times bigger are bacteria than viruses? 100x A cell is around how many times bigger than an individual bacteria? 10x Cell structures Know the function, general location, and whether they are shared with eukaryotes (if yes similarities/differences) of the following structures: 1. Nucleoid: contains chromosome(s) and nuclear-associated proteins that are usually haploid and circular near the center of the cell, which hold the DNA genetic information without a membrane Eukaryotes = have a nucleus, are diploid and linear Prokaryotes = have nucleoid, are haploid and circular 2. Ribosomes: work with mRNA protein synthesis, made up of proteins and RNA, found in cytoplasm Eukaryotes: 80S, 60 large, 40 small (18S sequencing) Prokaryotes: 70S, 50 large, 30 small (16S sequencing) 3. Cytoplasm: fluid inner layer 4. Fimbriae: short, bristle-like projections for attachment to surfaces 5. Endospores: not all bacteria have, protect bacteria in a dormant state/ harsh environment, found inside bacteria, sporulation = the process of becoming dormant, germination = process of becoming active 6. Plasma membrane: semipermeable, composed of lipids and proteins, controls transport into and out of cell, most inner layer before cytoplasm (ex: facilitated diffusion, active transport, diffusion, endocytosis (Eukaryotes), sterols (Eukaryotes), and cholesterol (Eukaryotes) Same for Eukaryotes 7. Cell wall: protects against harsh changing environments and osmotic stress, contains peptidoglycan in bacteria, contains Gram + and Gram - Steps of Gram stain: dye with crystal violet, iodine (mordant), alcohol (decolorizer), and safranin red If mycobacteria complete an acid-fast stain Gram + has LTA an TA and one membrane Gram - has LPS and 2 membranes 8. Capsule: protective protein shell, outermost layer 9. Pilus: medium projection, adheres to surfaces, does DNA gene transfer 10. Flagellum: long protein projections made of flagellin for movement (ex: 1= monotrichous, one at each end = amphitrichous, many at one end = lophotrichous, many flagella all over the cell = peritrichous 11. Plasmid: circular, double-stranded DNA not part of the chromosome, and can have 1-100 of the same or different plasmids to help with gene transfer, antibiotic resistance, and virulence factors, found anywhere in the cytoplasm Not part of Eukaryotes 12. Inclusion: not all bacteria have, helps with the storage of nutrients and other materials, has protein shell, and is found in cytoplasm (ex: lipid droplets store fats, volutin stores inorganic phosphates, sulfur inclusions store sulfur, gas bubbles store gas for buoyancy in water, magnetosomes store metals for movement) Not part of Eukaryotes; instead, they have vesicles for storage All cellular microbes have what four (4) components? Cell Membrane, Cytoplasm, Ribosomes, and DNA What is the only component of all cells that viruses have? Cytoplasm Know/ be able to identify the shapes of bacteria Round shape: coccus Rod shape: bacillus Vibrio: curved rod shape Short rods with combo of coccus and bacillus: coccobacillus Wavy spiral shape: Spirillum Coiled spiral shape: Spirochete Microbial growth Phases of growth Lag Phase: bacteria gear up for replication by increasing cell size, metabolism, and protein synthesis Log Phase: exponential growth phase, where bacteria actively replicate and are most susceptible to antibiotics Stationary Phase: growth curve flatlines bc bacteria are dying at same rate of growth, begin survival mode and sporulation, produce secondary metabolites, and produce virulence factors, and are low on space, nutrients, and oxygen Death Phase: bacteria die at exponential rate, increase amount of toxic waste, release spores, some spontaneously lyse to feed others, and persister cells refuse to die How do microbes replicate? Through binary fission, fragmentation, budding, and sexually What is a biofilm? Communities of bacteria (steps: colonization, attachment, replication, make EPS with antibiotic resistance, EPS kick bacteria out to replicate somewhere else) How does quorum sensing work? Bacteria want to work together, so they secrete autoinducers, and when you activate enough receptors, they activate a response Growth requirements – classifications and adaptations 1. Oxygen requirements Obligate Aerobes: need O2 to survive Obligate Anaerobes: die in presence of O2 Faculative: mostly need O2 but can survive without it Microaerophiles: need O2 for survival but not atmospheric O2 (low levels) Aerotolerant: can survive with or without oxygen Capnophiles: like high CO2 and low O2 2. pH requirements Acidophiles: low pH (2-4), high H+ environments, efflux pumps to remove H+ ions, changes membrane composition to withstand low pH Neutrophiles: neutral pH (7), found within body Alkaliphiles: basic pH (9-10), modified lipid protein structures, modified electron transport system that use Na+ instead of H+, high OH- environment 3. temperature requirements Psychrophiles: like freezing temperatures a below 0-15 degrees, die at or above 20 degrees, found in cold lakes or the ocean floor, have hydrophobic proteins to increase flexibility, have decreased secondary stabilizing bonds Psychrotolerant: cold not ideal but wont kill them, live between 4-25 degrees (fridge temperature) Mesophiles: moderate temperatures, 20-40 degrees, grow in body Thermophiles: hot environments 50-80 degrees, hot springs, geothermal soil Hyperthermophiles: very hot environments 80-110 degrees, found in hydrothermal vents, increased saturation in membranes, increased stabilizing bonds, alter amino acids to prevent denaturation 4. osmolarity requirements Hypertonic: more water out Hypotonic: more water in Isotonic: equal water in and out Halotolerant: dont require salt but can grow in high salt environments Halophiles: love salt, found in ocean and salt lakes, have increased cytoplasmic glycerol, have efflux pumps for salt 5. barometric requirements Barophile: survive high atmospheric environments like the bottom of the ocean (something at top of mountain has low atmospheric pressure) Microbial Metabolism Means of generating energy (do the processes require oxygen?, which gives the most energy?) Glycolysis: does not require O2 bc it can be used during fermentation, used 2 ATP, makes 4 ATP, NET 2 ATP Kreb’s cycle: requires O2; NET after 1 round= 1ATP, 1 NADH, 1FADH2 (x2 for second round) Electron transport: requires O2, biggest payout of ATP with NET 34 ATP Photosynthesis: does not require O2 as it is a waste product, can do oxygenic and anoxygenic Microbial genetics DNA Replication – enzymes and functions DNA gyrase: unwinds DNA (enzyme) Helicase: unzips DNA (enzyme) Single-stranded binding proteins: bind to DNA so doesnt close back up (protein) Single-stranded binding proteins: bind to DNA so doesnt close back up (protein) DNA polymerase III: lay down new DNA nucleotides, synthesizes leading and lagging strands 5’ →3’(enzyme) DNA polymerase I: removes RNA primers (enzyme) DNA Ligase: seals RNA primers (enzyme) Topoisomerase 4: separates 2 circular chromosomes Transcription and translation – enzymes and function 1. RNA polymerase: turns DNA→ mRNA (transcription) 2. Ribosomes: 30S small, 50S large, 70S total 3. tRNA: A,P, and E sites, bacteria links transcription and translation bc no nucleus, has anticodon at opposite long 3’ end, high energy bond, same active translation process as Eukaryotes Be able to do DNA base pairing, transcription, and translation (given codon table) Also know differences between prokaryotic and eukaryotic Replication, transcription, and translation Genetic Diversity – how does this work? 1. Transformation: uptake of plasmid into different bacteria and is incorporated into its genetic material 2. Transduction: bacteriophage inserts its plasmid into a bacteria (virus that infects a bacteria) 3. Conjugation: like plasmid transfer (ex: rolling circle replication), “bacterial sex” 4

Receptor Mediated Endocytosis

endocytosis 4

endocytosis 3

Topic 14: Endocytosis and Degradation by the Lysosome

endocytosis 2

endocytosis 1

Endocytosis

BS2092 - Endocytosis

Endocytosis & Exocytosis

Endocytosis and Lysosomal Pathway

CBIO3400 Lecture L19 - Retrograde Transport and Exocytosis

Endocytosis and intracellular vesicle traffic lecture