exocytosis and lysosome

What coat protein allows exocytosis from trans golgi on

From golgi to go to lysosome, endososmes and to pm there is CLATERIN

A part from transport what is the function of clatherin in maturation of the vescicle

Clatherin removes unecessary immature protein so that the scretory vescicle has an AGGREGATED OF CARGO PROTEIN AND IS MATURE

How does vescicle maturation happens after there is the budding in the trans golgi?

The budding forms in the trans golgi

Cargo protein aggregates and the lymen of the trans golgi is more ioninc

Clatherin attaches

Maturation and removal of immature proteins

Why do we have different pepetidehormons from one single pro hormOne

Because the precursore is cleaved to produce different kinds of hormons

What allows the fusion of synaptic vescicle wehn needed with the pm

Snare prtreoins that are partially assembled and blocked until you need to secrete

What is priming and who inhibits snare?

Priming is when snare are partially assembled but are blocked from fusing the synaptic vescicle with the pm temporarly by COMPLEXINS stabilizing the snare complez

What is the role of complexins

snare are partially assembled but are blocked from fusing the synaptic vescicle with the pm temporarly by COMPLEXINS stabilizing the snare complez

If then the synaptic contet has to be released how is the snare complex re-activated?

Calcium ions trigger SYNAPTOTAGMIN binding to phopholipids and to SNARE these bindings make complexin displace and so the synaptic conent is released

How is complexin moved away from snare

CALCIUM MAKES synaptoTEGMIN bind to phopholipids and to SNARE

If there is no calcium can you have fusion of a secretory vesicle

NO

What is the function of the V type proton pump in the lysosomal memebrane?

Creates the acidic internal ph

How does the v pupm work

The h+ ions alone are not sufficient there has to be an equal amount of ANION in the same direction!!!

How are lysosomal protein modified and where

Lysosomal protein are modified in the CIS GOLGI

By phophorylation of MANNOSE on n glycosoilayion

The lysosomal enzymes are synthetzed in the er

In the golgi mannose residues are PHOPHORYLATED to M6P ( mannose 6 phophate) mediated by GlcNac P

Then lysosomal hydrolase with GlcNac p attched to mannose

GlcNac p detaches

You have you lysosomal hydrolases with mannose phophorylated m6p

What does GlcNac phophotransferase do

Recognize a structural signal ( signal patches) on. Lyssonmal protein ( M6P

What are the steps that lead lysosomal hydrolase to then be inserted into the eraly endosome?

1. You have in cis the lyosome hydrilase precursore and your mannose residue

2. P glcNA associates to the mannose and lys. Hyrsolase precursors

3. Uncovering of the mannose 6 phophate signals that PUTS THE LYSOSOME IN THE TRANS

4. Clatherin coat

5. Tranport to endosome

6. M6p recptor in budding vesciles

7. Entry in early endosome and phophate removal

Which is the signals that leads the precursor of lysosomal hydrolosa to go to the trans goldi and then to the early endosome

MANNOSE 6 PHOPHate

What is the cause of tay sachs disease

Lyssome disease lead to accumulation of undegradated material

These are SYSTEM DISSEASES

1. Mutation of HEXOSAMINIDASE in tay sacs disease does not allow the phophorylation of mannose and so lysosome are not localized!!!!!

2. Tay sacs disease is caracterized by undigested ganaglioside GM2 that interfears with the normal functioning of the brain

For each say what they recieve and where to the adress

1. Recycling endosome

Recieve from early endosome materials and they transported to PM

2. EARLY ENDOSOMES REC. FROM endocyting vescile from the trans golgi AND SEND TO recyling endosome Or they mature into late

3. Late endosome formed from multivescicular bodies and then fuse with the vescicle form trans golgi that carry lyssome hydrolases and become lysosomes

How do late endosome become lysosomes

They recieve all the molecules from the mulltivescicular bodiues and fuse with the lysosomal hydrolase ( m6p signale) from the TGN