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BIOL 101 Complete Study Guide Unit 1: DNA, RNA, and Genetics 1. DNA structure What is DNA? DNA stands for deoxyribonucleic acid. It stores the genetic information that tells cells how to function, grow, and reproduce. What is the shape of DNA? DNA has a double-helix shape, which looks like a twisted ladder. What are the building blocks of DNA? Nucleotides. What are the three parts of a DNA nucleotide? * A phosphate group * A five-carbon sugar called deoxyribose * A nitrogenous base What are the four nitrogenous bases in DNA? * Adenine (A) * Thymine (T) * Cytosine (C) * Guanine (G) What are the DNA base-pairing rules? * Adenine pairs with Thymine (A–T). * Cytosine pairs with Guanine (C–G). What does complementary base pairing mean? Each DNA base has a specific partner. Knowing one DNA strand allows you to determine the matching strand. Example: * Original: A T C G * Complement: T A G C What holds the two DNA strands together? Hydrogen bonds between the nitrogenous bases. 2. DNA vs. RNA What is RNA? RNA stands for ribonucleic acid. It helps use the genetic instructions stored in DNA to make proteins. What are the main differences between DNA and RNA? DNA RNA Usually double-stranded Usually single-stranded Contains deoxyribose sugar Contains ribose sugar Uses thymine (T) Uses uracil (U) Stores genetic information Helps make proteins What are the four bases in RNA? * Adenine (A) * Uracil (U) * Cytosine (C) * Guanine (G) What replaces thymine in RNA? Uracil. What are the three main types of RNA? * mRNA (messenger RNA): Carries genetic instructions from DNA to the ribosome. * tRNA (transfer RNA): Brings amino acids to the ribosome. * rRNA (ribosomal RNA): Makes up part of the ribosome and helps build proteins. 3. DNA replication What is DNA replication? DNA replication is the process of making an identical copy of DNA before a cell divides. Why is DNA replication important? It ensures that each new cell receives a copy of the genetic information. What does semiconservative replication mean? Each new DNA molecule contains one original strand and one newly made strand. What enzyme unzips DNA? Helicase. What enzyme adds new DNA nucleotides? DNA polymerase. What enzyme connects DNA fragments on the lagging strand? DNA ligase. What is the leading strand? The strand that is copied continuously in the direction of the replication fork. What is the lagging strand? The strand that is copied in short sections called Okazaki fragments. What are Okazaki fragments? Short pieces of newly made DNA that form on the lagging strand. What is the job of DNA ligase? It joins the Okazaki fragments together. What are telomeres? Protective sections of DNA found at the ends of chromosomes. What is the purpose of telomeres? They help protect important genetic information from being lost when DNA is copied. What is DNA repair? The process cells use to identify and correct mistakes or damage in DNA. 4. Transcription What is transcription? Transcription is the process of copying genetic information from DNA into mRNA. Where does transcription happen in eukaryotic cells? In the nucleus. What enzyme helps make RNA during transcription? RNA polymerase. What is the purpose of transcription? To create an mRNA copy of a gene that can be used to make a protein. What are the RNA base-pairing rules? * DNA A pairs with RNA U. * DNA T pairs with RNA A. * DNA C pairs with RNA G. * DNA G pairs with RNA C. Example: DNA: A T C G RNA: U A G C What happens to mRNA before it leaves the nucleus in eukaryotic cells? It undergoes RNA processing. What are introns? Sections of RNA that are removed during RNA processing. What are exons? Sections that remain and are joined together to form mature mRNA. What is RNA splicing? The removal of introns and joining of exons. 5. Translation What is translation? Translation is the process of using mRNA instructions to build a protein. Where does translation happen? At the ribosomes in the cytoplasm or on the rough endoplasmic reticulum. What is a codon? A group of three mRNA bases that codes for an amino acid or a stop signal. What is an anticodon? A group of three bases on tRNA that pairs with a matching mRNA codon. What is the role of tRNA? It carries amino acids to the ribosome. What is the role of the ribosome? It reads mRNA and connects amino acids to build a protein. What is the start codon? AUG. What amino acid does AUG code for? Methionine. What are the three stop codons? * UAA * UAG * UGA What happens when a stop codon is reached? Translation ends, and the completed protein is released. 6. Gene expression and regulation What is gene expression? Gene expression is the process of using information from a gene to make a functional product, such as a protein. What is gene regulation? Gene regulation controls when, where, and how much a gene is expressed. Why is gene regulation important? It allows cells to perform different functions even though many cells contain the same DNA. What is an operon? A group of genes controlled together, commonly found in bacteria. What is the lac operon? A group of bacterial genes involved in breaking down lactose. What happens when lactose is present? Lactose helps remove the repressor’s blockage, allowing the genes to be expressed. What happens when lactose is absent? The repressor binds to the operator and prevents the genes from being expressed. Unit 2: Evolution and Natural Selection 1. Understanding evolution What is evolution? Evolution is the change in inherited characteristics of a population over generations. Does an individual organism evolve during its lifetime? No. Populations evolve over generations. What is a population? A group of individuals of the same species living in the same area. What is a species? A group of organisms that can reproduce with one another and produce fertile offspring, under the biological species concept. What is natural selection? Natural selection is the process in which organisms with inherited traits that improve survival or reproduction tend to leave more offspring. Who is associated with the theory of evolution by natural selection? Charles Darwin. What is adaptation? An inherited characteristic that helps an organism survive or reproduce in its environment. What is biological fitness? An organism’s ability to survive and reproduce, measured by its contribution of offspring to the next generation. Does the strongest organism always have the highest fitness? No. Fitness depends on how well an organism’s inherited traits help it reproduce in its particular environment. 2. Natural selection What are the main requirements for natural selection? * Individuals in a population have variation. * Some variation is inherited. * Organisms produce more offspring than can survive. * Individuals with certain inherited traits reproduce more successfully. What happens to helpful inherited traits over generations? They may become more common in the population. Can natural selection create traits because organisms need them? No. Natural selection acts on existing inherited variation. It does not create a needed trait simply because an organism needs it. What is an example of natural selection? If insects vary in color and birds more easily spot one color, insects with the less visible color may survive and reproduce more often. Over generations, that color may become more common. 3. Population genetics What is an allele? An alternative version of a gene. What is a gene pool? All the alleles present in a population. What is allele frequency? The proportion of a particular allele in a population. What does it mean when allele frequencies change? The population is evolving. What is genetic variation? Differences in genetic information among individuals in a population. Why is genetic variation important? It provides the differences that natural selection can act on. 4. Mechanisms of evolution What are the five major mechanisms that can change allele frequencies? * Mutation * Gene flow * Genetic drift * Natural selection * Nonrandom mating can change genotype frequencies and, in some circumstances, influence allele frequencies indirectly through selection. What is mutation? A change in DNA sequence. How can mutations affect evolution? They can introduce new genetic variations and sometimes create new alleles. What is gene flow? The movement of alleles between populations when individuals or their reproductive cells move between them. What is genetic drift? A random change in allele frequencies, especially noticeable in small populations. What is the bottleneck effect? A type of genetic drift that happens when a population becomes much smaller due to a random event. What is the founder effect? A type of genetic drift that occurs when a small group separates from a larger population and starts a new population. What is the difference between natural selection and genetic drift? Natural selection is based on differences in reproductive success associated with inherited traits. Genetic drift changes allele frequencies randomly. 5. Hardy-Weinberg equilibrium What is Hardy-Weinberg equilibrium? A model that describes a population that is not evolving with respect to the gene being studied. What are the five Hardy-Weinberg conditions? * No mutations * Random mating * No natural selection * Very large population * No gene flow What is the Hardy-Weinberg equation? p+q=1 What does p represent? The frequency of one allele. What does q represent? The frequency of the other allele. What is the genotype frequency equation? p^2+2pq+q^2=1 What does each part represent? * : Homozygous dominant * : Heterozygous * : Homozygous recessive What does homozygous mean? Having two identical alleles for a gene. What does heterozygous mean? Having two different alleles for a gene. Practice question: If q = 0.4, what is p? Answer: p=1-0.4=0.6 Practice question: If p = 0.6 and q = 0.4, what is the frequency of homozygous dominant individuals? Answer: p^2=0.6^2=0.36 Practice question: If p = 0.6 and q = 0.4, what is the frequency of heterozygous individuals? Answer: 2pq=2(0.6)(0.4)=0.48 Practice question: If p = 0.6 and q = 0.4, what is the frequency of homozygous recessive individuals? Answer: q^2=0.4^2=0.16 6. Evidence for evolution What are some forms of evidence for evolution? * Fossil records * Comparative anatomy * Molecular similarities * Embryology * Biogeography What are fossils? Preserved remains or traces of organisms that lived in the past. What is homologous anatomy? Similar body structures inherited from a common ancestor, even if they serve different functions. What is an example of homologous structures? The forelimbs of humans, whales, bats, and cats. What is analogous anatomy? Structures that perform similar functions but evolved independently and do not come from the same ancestral structure. What is an example of analogous structures? The wings of birds and insects. What is biogeography? The study of where organisms live and how their geographic distribution relates to evolution. How can DNA provide evidence for evolution? Organisms with more similar DNA sequences often share a more recent common ancestor. 7. Speciation What is speciation? The formation of new species. What is reproductive isolation? When populations can no longer successfully reproduce with one another. What is geographic isolation? When physical barriers separate populations, such as mountains, rivers, or oceans. What is allopatric speciation? Speciation that occurs when populations are geographically separated. What is sympatric speciation? Speciation that occurs without geographic separation. Unit 3: Classification, Taxonomy, and Phylogeny 1. Classification What is taxonomy? The science of naming and classifying organisms. What is systematics? The study of biological diversity and evolutionary relationships. What is binomial nomenclature? The two-part scientific naming system for organisms. What are the two parts of a scientific name? Genus and species. How should scientific names be written? The genus is capitalized, the species name is lowercase, and both are italicized. Example: Homo sapiens. What are the main taxonomic levels from broadest to most specific? * Domain * Kingdom * Phylum * Class * Order * Family * Genus * Species What are the three domains of life? * Bacteria * Archaea * Eukarya 2. Phylogenetic trees What is a phylogenetic tree? A diagram showing evolutionary relationships among organisms. What does a branch point represent? A common ancestor where evolutionary lineages split. What is a common ancestor? An ancestral organism or population from which two or more groups evolved. What are sister taxa? Two groups that share an immediate common ancestor. What does a node represent on a phylogenetic tree? A branching point representing a common ancestor. Does being next to another organism on a tree always mean they are closely related? No. Relationships are determined by shared branch points, not simply by how close the names appear. What is a clade? An ancestor and all of its descendants. What is a derived trait? A trait that evolved in a particular lineage and can help identify relationships among organisms. Unit 4: Prokaryotes, Eukaryotes, Bacteria, and Archaea 1. Cell types What are prokaryotic cells? Cells that do not have a nucleus or membrane-bound organelles. What are eukaryotic cells? Cells that have a nucleus and membrane-bound organelles. What organisms are prokaryotes? Bacteria and Archaea. What organisms are eukaryotes? Animals, plants, fungi, and protists. What is the main difference between prokaryotic and eukaryotic cells? Eukaryotic cells have a nucleus and membrane-bound organelles. Prokaryotic cells do not. Where is DNA located in a prokaryotic cell? In a region called the nucleoid. What is the function of the cell membrane? It controls what enters and leaves the cell. What is the function of ribosomes? They make proteins. 2. Bacteria and Archaea What are bacteria? Single-celled prokaryotic organisms found in many environments. What are Archaea? Single-celled prokaryotic organisms that are genetically and chemically distinct from bacteria. What is binary fission? A form of asexual reproduction in which one cell divides into two cells. Do bacteria and archaea have a nucleus? No. What is a bacterial cell wall commonly made of? Peptidoglycan. What is peptidoglycan? A material that provides structure and support to bacterial cell walls. 3. Gram-positive and Gram-negative bacteria What are Gram-positive bacteria? Bacteria with a thick peptidoglycan cell wall that usually stain purple during Gram staining. What are Gram-negative bacteria? Bacteria with a thinner peptidoglycan layer and an outer membrane that usually stain pink or red during Gram staining. What is the major difference between Gram-positive and Gram-negative bacteria? Gram-positive bacteria have thick peptidoglycan walls, while Gram-negative bacteria have thin peptidoglycan walls and an outer membrane. 4. Endosymbiotic theory What is the endosymbiotic theory? The theory that mitochondria and chloroplasts originated from free-living bacteria that were taken into ancestral eukaryotic cells. What organelles are explained by endosymbiotic theory? Mitochondria and chloroplasts. What evidence supports endosymbiotic theory? * They contain their own DNA. * They have their own ribosomes. * They divide in a way similar to bacteria. * They have double membranes. What is the function of mitochondria? They produce most of the cell’s ATP through cellular respiration. What is the function of chloroplasts? They carry out photosynthesis in plants and some other organisms. 5. Protists and fungi What are protists? A diverse group of eukaryotic organisms that do not fit into the traditional plant, animal, or fungi kingdoms. Are all protists single-celled? No. Many are single-celled, but some are multicellular. What are fungi? Eukaryotic organisms that absorb nutrients from their surroundings. What is the cell wall of fungi made of? Chitin. What are examples of fungi? * Mushrooms * Molds * Yeasts How do fungi obtain nutrients? They release digestive enzymes into their surroundings and absorb the broken-down nutrients. What is the role of decomposers? They break down dead organisms and recycle nutrients into the environment. Unit 5: Plants 1. Plant characteristics What are the major characteristics of plants? Plants are multicellular eukaryotes that generally have chloroplasts, cellulose cell walls, and carry out photosynthesis. What is photosynthesis? The process of using light energy to convert carbon dioxide and water into sugars and oxygen. What is the function of chlorophyll? It absorbs light energy for photosynthesis. What is the function of roots? They absorb water and minerals and help anchor the plant. What is the function of stems? They support the plant and transport materials. What is the function of leaves? They are the main organs where photosynthesis occurs in most plants. 2. Alternation of generations What is alternation of generations? A plant life cycle that alternates between two multicellular stages: the haploid gametophyte and the diploid sporophyte. What is a gametophyte? The haploid stage that produces gametes. What is a sporophyte? The diploid stage that produces spores through meiosis. What does haploid mean? Having one set of chromosomes, represented by . What does diploid mean? Having two sets of chromosomes, represented by . What is fertilization? The joining of two gametes to form a diploid zygote. What is meiosis? A type of cell division that reduces the chromosome number by half and produces haploid cells. What is the difference between spores and gametes? Spores can develop into a new organism without fusing with another cell. Gametes must fuse during fertilization to form a zygote. 3. Major plant groups What are the major groups of plants? * Nonvascular plants * Seedless vascular plants * Gymnosperms * Angiosperms What are nonvascular plants? Plants that lack specialized vascular tissues for transporting water and nutrients. What are examples of nonvascular plants? Mosses and liverworts. What are vascular plants? Plants that have specialized tissues to transport water, minerals, and sugars. What are the two major vascular tissues? * Xylem * Phloem What is the function of xylem? It transports water and minerals from the roots toward the rest of the plant. What is the function of phloem? It transports sugars and other organic nutrients throughout the plant. What are seedless vascular plants? Vascular plants that reproduce using spores instead of seeds. What is an example of a seedless vascular plant? Ferns. What are gymnosperms? Seed-producing plants with seeds that are not enclosed within fruits. What is an example of a gymnosperm? Pine trees. What are angiosperms? Flowering plants that produce seeds enclosed within fruits. What is the main difference between gymnosperms and angiosperms? Gymnosperms have exposed seeds, while angiosperms produce seeds enclosed in fruits. 4. Plant reproduction What is pollination? The transfer of pollen from the male reproductive structure to the female reproductive structure of a plant. What is pollen? A structure that carries the male gametophyte in seed plants. What is the purpose of flowers? Flowers are reproductive structures of angiosperms. What develops into a fruit? The ovary of a flower. What develops into a seed? The ovule after fertilization. What is the purpose of seeds? They protect and provide resources for the developing plant embryo. Unit 6: Animals 1. Animal characteristics What are the major characteristics of animals? Animals are multicellular eukaryotes that obtain nutrients by consuming other organisms or organic material. They do not have cell walls. Are animals prokaryotic or eukaryotic? Eukaryotic. Are animals unicellular or multicellular? Multicellular. Are animals autotrophs or heterotrophs? Heterotrophs. What is a heterotroph? An organism that obtains energy and nutrients by consuming other organisms or organic material. 2. Animal body plans What is symmetry? The arrangement of body parts around an axis or center. What is asymmetry? A body shape that cannot be divided into matching halves. What is radial symmetry? A body arrangement in which body parts are organized around a central axis. What is an example of an animal with radial symmetry? A jellyfish. What is bilateral symmetry? A body arrangement that can be divided into approximately equal left and right halves. What is an example of an animal with bilateral symmetry? A human. What is cephalization? The concentration of sensory organs and nervous tissue toward the front or head region of an animal. What is a body cavity? A fluid-filled space within an animal’s body that can contain and protect internal organs. 3. Major animal groups What are sponges? Simple aquatic animals that lack true tissues and organs. What is the scientific phylum for sponges? Porifera. How do sponges obtain food? They filter small food particles from water flowing through their bodies. What are cnidarians? Aquatic animals with specialized stinging cells. What is the scientific phylum for cnidarians? Cnidaria. What are examples of cnidarians? * Jellyfish * Corals * Sea anemones What are cnidocytes? Specialized stinging cells used for capturing prey and defense. What are arthropods? Animals with segmented bodies, jointed appendages, and an external skeleton. What is an exoskeleton? A hard external structure that supports and protects an animal’s body. What are examples of arthropods? * Insects * Spiders * Crabs * Lobsters What is molting? The process of shedding an old exoskeleton so an arthropod can grow. What are the major animal groups covered in this section? * Porifera: Sponges * Cnidaria: Jellyfish, corals, and sea anemones * Arthropoda: Insects, spiders, and crustaceans Note: Your lecture material did not provide a complete list of characteristics for every animal phylum, so I have not added unsupported details to those sections. Unit 7: Biotechnology 1. Biotechnology basics What is biotechnology? The use of living organisms, cells, or biological processes to develop useful products and technologies. What is genetic engineering? The process of directly changing an organism’s DNA. What is recombinant DNA? DNA made by combining genetic material from different sources. What is a plasmid? A small circular DNA molecule commonly found in bacteria. Why are plasmids useful in biotechnology? They can be used to carry selected genes into cells. 2. PCR What does PCR stand for? Polymerase chain reaction. What is PCR used for? To make many copies of a specific DNA segment. What are the three main stages of PCR? * Denaturation * Annealing * Extension What happens during denaturation? The DNA strands separate. What happens during annealing? Primers attach to complementary DNA sequences. What happens during extension? DNA polymerase adds nucleotides to build new DNA strands. 3. Gel electrophoresis What is gel electrophoresis? A laboratory technique used to separate DNA fragments based on size. How does gel electrophoresis separate DNA? An electric current moves DNA fragments through a gel. Smaller fragments generally travel farther than larger fragments. Why does DNA move toward the positive end of the gel? DNA has a negatively charged phosphate backbone. What can gel electrophoresis help scientists do? Compare DNA fragments and examine DNA samples. 4. Restriction enzymes and cloning What are restriction enzymes? Enzymes that cut DNA at specific sequences. What is DNA cloning? The process of making copies of a DNA segment or gene. What is a GMO? A genetically modified organism whose genetic material has been altered using genetic engineering or other genetic modification methods. What is a transgenic organism? An organism that contains genetic material introduced from another species. What is CRISPR? A gene-editing technology that allows scientists to make targeted changes to DNA. What is gene therapy? A medical approach that aims to treat or prevent disease by changing genetic material in a person’s cells. What is genomics? The study of an organism’s entire genome. What is proteomics? The study of the proteins produced by a cell, tissue, or organism. Practice Questions: Multiple Choice The answer is immediately underneath each question. Try answering before reading it. 1. What is the basic building block of DNA? A. Amino acid B. Nucleotide C. Protein D. Lipid Answer: B. Nucleotide 2. Which base pairs with adenine in DNA? A. Guanine B. Cytosine C. Thymine D. Uracil Answer: C. Thymine 3. Which enzyme separates DNA strands during replication? A. Ligase B. Helicase C. RNA polymerase D. Ribosome Answer: B. Helicase 4. What is the purpose of DNA replication? A. To create proteins B. To make RNA C. To copy DNA before cell division D. To destroy old DNA Answer: C. To copy DNA before cell division 5. What is the process of making mRNA from DNA called? A. Translation B. Transcription C. Replication D. Mutation Answer: B. Transcription 6. Where does translation occur? A. Nucleus B. Ribosome C. Cell membrane D. Golgi apparatus Answer: B. Ribosome 7. What does tRNA carry? A. DNA B. Glucose C. Amino acids D. Lipids Answer: C. Amino acids 8. What is the start codon? A. UAA B. AUG C. UGA D. UAG Answer: B. AUG 9. What is evolution? A. An individual changing during its lifetime B. A population changing genetically over generations C. An organism growing larger D. A cell dividing Answer: B. A population changing genetically over generations 10. Who is associated with evolution by natural selection? A. Gregor Mendel B. Charles Darwin C. Louis Pasteur D. Robert Hooke Answer: B. Charles Darwin 11. What is genetic drift? A. A random change in allele frequencies B. The movement of water C. The creation of proteins D. The copying of DNA Answer: A. A random change in allele frequencies 12. What is gene flow? A. DNA replication B. Movement of alleles between populations C. Protein synthesis D. Natural death Answer: B. Movement of alleles between populations 13. Which equation represents Hardy-Weinberg allele frequencies? A. B. C. D. Answer: B. 14. What are the three domains of life? A. Plants, animals, fungi B. Bacteria, Archaea, Eukarya C. Mammals, reptiles, birds D. Protists, fungi, plants Answer: B. Bacteria, Archaea, Eukarya 15. Which cells do not have a nucleus? A. Eukaryotic B. Prokaryotic C. Animal D. Plant Answer: B. Prokaryotic 16. What is the function of mitochondria? A. Protein storage B. ATP production C. DNA protection D. Photosynthesis Answer: B. ATP production 17. What is the cell wall of fungi made of? A. Cellulose B. Peptidoglycan C. Chitin D. Starch Answer: C. Chitin 18. What tissue transports water in plants? A. Phloem B. Xylem C. Epidermis D. Pollen Answer: B. Xylem 19. What is the function of phloem? A. Transport sugars B. Absorb sunlight C. Produce pollen D. Protect seeds Answer: A. Transport sugars 20. Which plants produce flowers? A. Mosses B. Ferns C. Gymnosperms D. Angiosperms Answer: D. Angiosperms 21. What is the scientific phylum for sponges? A. Cnidaria B. Porifera C. Arthropoda D. Chordata Answer: B. Porifera 22. What do arthropods have? A. Jointed appendages and an exoskeleton B. No body structure C. Only soft bodies D. Chloroplasts Answer: A. Jointed appendages and an exoskeleton 23. What is PCR used for? A. Separating proteins B. Copying DNA C. Producing ATP D. Growing plants Answer: B. Copying DNA 24. What does gel electrophoresis separate DNA by? A. Color B. Shape only C. Fragment size D. Temperature Answer: C. Fragment size 25. What is CRISPR used for? A. Editing DNA B. Separating cells C. Making ATP D. Producing pollen Answer: A. Editing DNA Fill-in-the-Blank Practice 1. DNA stands for __________. Answer: Deoxyribonucleic acid. 2. RNA uses __________ instead of thymine. Answer: Uracil. 3. The enzyme that unzips DNA is __________. Answer: Helicase. 4. The enzyme that connects Okazaki fragments is __________. Answer: DNA ligase. 5. The process of making RNA from DNA is called __________. Answer: Transcription. 6. The process of making proteins from mRNA is called __________. Answer: Translation. 7. A group of three mRNA bases is called a __________. Answer: Codon. 8. The movement of alleles between populations is called __________. Answer: Gene flow. 9. Random changes in allele frequencies are called __________. Answer: Genetic drift. 10. The formation of new species is called __________. Answer: Speciation. 11. The three domains are Bacteria, Archaea, and __________. Answer: Eukarya. 12. The cell wall of fungi contains __________. Answer: Chitin. 13. The plant tissue that transports water is __________. Answer: Xylem. 14. The plant tissue that transports sugars is __________. Answer: Phloem. 15. Flowering plants are called __________. Answer: Angiosperms. 16. The haploid plant stage is called the __________. Answer: Gametophyte. 17. The diploid plant stage is called the __________. Answer: Sporophyte. 18. Sponges belong to the phylum __________. Answer: Porifera. 19. Jellyfish belong to the phylum __________. Answer: Cnidaria. 20. The process used to make many copies of DNA is called __________. Answer: PCR. Important Terms to Memorize Term Meaning DNA Stores genetic information RNA Helps make proteins Nucleotide Building block of DNA and RNA Helicase Separates DNA strands DNA polymerase Builds new DNA DNA ligase Connects DNA fragments Transcription DNA to RNA Translation RNA to protein Codon Three mRNA bases Mutation Change in DNA Evolution Change in inherited traits in a population over generations Natural selection Differences in reproductive success based on inherited traits Genetic drift Random allele frequency change Gene flow Movement of alleles between populations Speciation Formation of new species Taxonomy Naming and classifying organisms Phylogeny Evolutionary relationships Prokaryote Cell without a nucleus Eukaryote Cell with a nucleus Mitochondria ATP production Chloroplast Photosynthesis Xylem Transports water Phloem Transports sugars Gametophyte Haploid plant generation Sporophyte Diploid plant generation Angiosperm Flowering plant Gymnosperm Plant with exposed seeds PCR Makes copies of DNA Gel electrophoresis Separates DNA fragments by size CRISPR Gene-editing technology Final Cram Sheet: What You Should Know Before Your Exam DNA and genetics * DNA: A–T and C–G * RNA: A–U and C–G * Helicase: unzips DNA * DNA polymerase: builds DNA * Ligase: joins DNA fragments * Transcription: DNA → RNA * Translation: RNA → protein * AUG: start codon * UAA, UAG, UGA: stop codons Evolution * Darwin: natural selection * Mutation: changes DNA * Genetic drift: random change * Gene flow: movement between populations * Bottleneck: population becomes much smaller * Founder effect: small group starts a population * Speciation: new species form * Hardy-Weinberg: , Classification and cells * Three domains: Bacteria, Archaea, Eukarya * Prokaryotes: no nucleus * Eukaryotes: have a nucleus * Mitochondria: ATP * Chloroplasts: photosynthesis * Fungi: chitin cell walls * Bacteria: peptidoglycan cell walls Plants * Xylem: water * Phloem: sugar * Gametophyte: haploid * Sporophyte: diploid * Gymnosperms: exposed seeds * Angiosperms: flowers and enclosed seeds * Mosses: nonvascular * Ferns: seedless vascular Animals and biotechnology * Porifera: sponges * Cnidaria: jellyfish, corals, sea anemones * Arthropoda: jointed appendages and exoskeleton * PCR: copies DNA * Gel electrophoresis: separates DNA by size * Restriction enzymes: cut DNA * CRISPR: edits DNA Study tip: Cover the answers with a piece of paper and try to answer every question from memory. Then uncover the answer and check yourself. Focus especially on DNA replication, transcription versus translation, Hardy-Weinberg equations, evolution mechanisms, plant life cycles, and the differences between prokaryotic and eukaryotic cells.
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binomials
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Binary Fission
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PRAXIS MUSIC 5113 STUDY GUIDE Music Content Knowledge PURPOSE This study guide reviews important concepts in music theory, music history, performance, aural skills, music education, notation, and technology. It is a study resource, not a guarantee of the exact questions that will appear on the exam. SECTION 1: MUSIC THEORY 1. THE MUSICAL ALPHABET The musical alphabet consists of seven letters: A, B, C, D, E, F, G After G, the letters begin again at A. A sharp raises a note by one semitone. A flat lowers a note by one semitone. A natural cancels a previous sharp or flat. A half step is the smallest interval in standard Western equal-tempered music. A whole step equals two half steps. Important natural half steps: E to F B to C Examples of whole steps: C to D F to G A to B 2. MAJOR SCALES The major scale follows this pattern: WHOLE, WHOLE, HALF, WHOLE, WHOLE, WHOLE, HALF C MAJOR: C, D, E, F, G, A, B, C Half steps occur between E and F and between B and C. G MAJOR: G, A, B, C, D, E, F-sharp, G D MAJOR: D, E, F-sharp, G, A, B, C-sharp, D F MAJOR: F, G, A, B-flat, C, D, E, F B-flat MAJOR: B-flat, C, D, E-flat, F, G, A, B-flat 3. KEY SIGNATURES Order of sharps: F, C, G, D, A, E, B Order of flats: B, E, A, D, G, C, F Common major keys: C major has zero sharps or flats. G major has one sharp, F-sharp. D major has two sharps, F-sharp and C-sharp. A major has three sharps, F-sharp, C-sharp, and G-sharp. E major has four sharps. B major has five sharps. F-sharp major has six sharps. C-sharp major has seven sharps. F major has one flat, B-flat. B-flat major has two flats, B-flat and E-flat. E-flat major has three flats. A-flat major has four flats. D-flat major has five flats. G-flat major has six flats. C-flat major has seven flats. Relative major and minor keys share the same key signature. Examples: C major and A minor have no sharps or flats. G major and E minor have one sharp. F major and D minor have one flat. 4. SCALE DEGREES Scale degree 1: Tonic Scale degree 2: Supertonic Scale degree 3: Mediant Scale degree 4: Subdominant Scale degree 5: Dominant Scale degree 6: Submediant Scale degree 7: Leading tone Scale degree 8: Tonic, one octave higher In C major: 1 = C 2 = D 3 = E 4 = F 5 = G 6 = A 7 = B 8 = C The tonic is the tonal center and often feels like home. The dominant is scale degree 5 and commonly resolves to the tonic. The leading tone is scale degree 7 and commonly resolves upward to the tonic. 5. INTERVALS An interval is the distance between two pitches. Perfect unison: C to C Minor second: C to D-flat Major second: C to D Minor third: C to E-flat Major third: C to E Perfect fourth: C to F Tritone: C to F-sharp Perfect fifth: C to G Minor sixth: C to A-flat Major sixth: C to A Minor seventh: C to B-flat Major seventh: C to B Perfect octave: C to the next C Intervals have a number and a quality. Interval numbers include second, third, fourth, fifth, sixth, seventh, and octave. Interval qualities include major, minor, perfect, augmented, and diminished. 6. TRIADS AND CHORDS A triad contains three notes, usually stacked in thirds. Major triad: Root, major third, perfect fifth. Example: C, E, G. Minor triad: Root, minor third, perfect fifth. Example: C, E-flat, G. Diminished triad: Root, minor third, diminished fifth. Example: C, E-flat, G-flat. Augmented triad: Root, major third, augmented fifth. Example: C, E, G-sharp. In a major key, the usual triad pattern is: I = major ii = minor iii = minor IV = major V = major vi = minor vii diminished = diminished Uppercase Roman numerals represent major chords in this pattern. Lowercase Roman numerals represent minor chords. The diminished symbol identifies a diminished chord. In C major, the I chord is C major and the V chord is G major. The V chord commonly resolves to the I chord. 7. RHYTHM AND NOTE VALUES Whole note = four quarter-note beats. Half note = two quarter-note beats. Quarter note = one beat. Eighth note = half a beat. Sixteenth note = one-quarter of a beat. These values assume a simple meter in which the quarter note receives one beat. A dot adds half the original note value. A dotted half note equals three quarter-note beats. A rest indicates silence for a specific duration. The beat is the underlying pulse of music. Rhythm is the organization of note durations and rests. Tempo is the speed of the beat. Meter organizes beats into recurring groups. A measure is a unit of music separated by bar lines. Syncopation emphasizes unexpected beats or subdivisions. 8. TIME SIGNATURES The top number indicates how many beats or counting units are grouped in a measure. The bottom number identifies the note value used as the unit. 2/4 usually has two quarter-note beats per measure. 3/4 usually has three quarter-note beats per measure. 4/4 usually has four quarter-note beats per measure. 6/8 usually has two main beats, each divided into three eighth notes. Simple meter divides each main beat into two. Compound meter divides each main beat into three. Examples of simple meter include 2/4, 3/4, and 4/4. Examples of compound meter include 6/8, 9/8, and 12/8. 9. MUSICAL TEXTURE Monophonic texture consists of one melodic line. Several people singing the same melody in unison can still be monophonic. Homophonic texture consists of a melody supported by accompaniment or harmony. Polyphonic texture consists of two or more independent melodic lines. Heterophonic texture consists of simultaneous variations of the same melody. 10. MUSICAL FORM Binary form: AB. Ternary form: ABA. Rondo form: A recurring main section alternates with contrasting sections. An example is ABACA. Theme and variations: A theme is repeated with changes. Strophic form: The same music is repeated for different verses. Through-composed form: The music continues with new material rather than repeating the same large sections. Sonata form commonly includes exposition, development, and recapitulation. 11. DYNAMICS, TEMPO, AND ARTICULATION pp = pianissimo, very soft. p = piano, soft. mp = mezzo-piano, moderately soft. mf = mezzo-forte, moderately loud. f = forte, loud. ff = fortissimo, very loud. Crescendo means gradually louder. Diminuendo means gradually softer. Allegro means fast and lively. Adagio means slow. Andante means a walking pace. Moderato means moderate speed. Presto means very fast. Staccato means short and detached. Legato means smooth and connected. Accent means a note receives emphasis. Fermata means a note or rest is prolonged as directed. SECTION 2: MUSIC HISTORY AND LITERATURE 1. MEDIEVAL PERIOD, APPROXIMATELY 500 TO 1400 Important characteristics: Gregorian chant Monophonic sacred music Early musical notation Development of organum, which added voices to chant Important composer: Hildegard of Bingen 2. RENAISSANCE PERIOD, APPROXIMATELY 1400 TO 1600 Important characteristics: Polyphony Imitative counterpoint Balanced vocal writing Growth of secular vocal music Important genres: Motet Madrigal Mass Important composers: Josquin des Prez Giovanni Pierluigi da Palestrina Thomas Morley A madrigal is a secular vocal composition associated particularly with the Renaissance. 3. BAROQUE PERIOD, APPROXIMATELY 1600 TO 1750 Important characteristics: Ornamentation Contrast Basso continuo Counterpoint Development of opera Elaborate musical expression Important genres: Fugue Suite Concerto Opera Oratorio Important composers: Johann Sebastian Bach: Known for fugues, counterpoint, and sacred music. George Frideric Handel: Known for Messiah and other oratorios. Antonio Vivaldi: Known for The Four Seasons and concertos. Claudio Monteverdi: Important in the development of early opera. 4. CLASSICAL PERIOD, APPROXIMATELY 1750 TO 1820 Important characteristics: Balance Clarity Symmetry Clear phrase structure Formal organization Important genres: Symphony Sonata String quartet Concerto Opera Important composers: Wolfgang Amadeus Mozart: Known for operas, symphonies, and concertos. Joseph Haydn: Known for symphonies and string quartets. Ludwig van Beethoven: Bridged the Classical and Romantic periods. Sonata form commonly consists of exposition, development, and recapitulation. 5. ROMANTIC PERIOD, APPROXIMATELY 1820 TO 1900 Important characteristics: Emotional expression Expanded orchestras Richer harmony Nationalism Program music Virtuosic performance Important composers: Franz Schubert: Known for art songs and lieder. Frédéric Chopin: Known for piano compositions. Pyotr Ilyich Tchaikovsky: Known for ballets and orchestral music. Richard Wagner: Known for music dramas and operas. Hector Berlioz: Known for orchestration and programmatic music. Franz Liszt: Known for piano virtuosity and symphonic poems. 6. TWENTIETH-CENTURY AND CONTEMPORARY MUSIC Impressionism: Associated with Claude Debussy and Maurice Ravel. Emphasizes musical color, timbre, and distinctive harmonic language. Atonality: Music that does not rely on a conventional tonal center. Arnold Schoenberg is an important figure. Neoclassicism: Uses or adapts features associated with earlier musical periods. Igor Stravinsky is associated with this movement. Minimalism: Uses repetition and gradual musical change. Important composers include Philip Glass and Steve Reich. Other topics: Jazz Blues Electronic music Film music Experimental music Global musical traditions 7. IMPORTANT MUSICAL GENRES Symphony: A large-scale orchestral work, often consisting of several movements. Concerto: A work featuring a soloist or soloists in contrast with an orchestra. Sonata: An instrumental composition, often in multiple movements. Fugue: A contrapuntal composition based on a subject introduced in different voices. Opera: A dramatic work presented primarily through singing with instrumental accompaniment. Oratorio: A large vocal work, often sacred, generally performed without the staging typical of opera. Art song or Lied: A solo vocal composition, usually with piano accompaniment. Mass: A musical setting of texts from the Christian liturgy. Motet: A choral genre, often sacred, whose characteristics vary by period. Madrigal: A secular vocal genre especially associated with the Renaissance. SECTION 3: WORLD MUSIC AND CULTURAL CONTEXT West African drumming traditions: May feature layered rhythms, interlocking patterns, call and response, and community participation. Indian classical music: Raga refers to a melodic framework. Tala refers to a rhythmic cycle. Indonesian gamelan: Ensembles often include gongs, metallophones, and other instruments. Japanese shakuhachi: An end-blown bamboo flute. Blues: An African American musical tradition associated with expressive phrasing, blue notes, and characteristic harmonic patterns. Jazz: Often includes improvisation, interaction among musicians, and swing, depending on the style. Latin American music: Includes many regional traditions shaped by Indigenous, African, and European influences. Important principle: Study the sound, instruments, cultural context, and function of each musical tradition. Avoid assuming that every culture uses Western notation, harmony, or musical structures. SECTION 4: INSTRUMENT FAMILIES 1. WOODWINDS Examples: Flute Piccolo Oboe English horn Clarinet Bass clarinet Bassoon Contrabassoon Saxophone The flute produces sound when air is blown across an opening. The clarinet uses a single reed. The oboe uses a double reed. The bassoon uses a double reed. The saxophone uses a single reed, even though it is commonly made of brass. 2. BRASS Examples: Trumpet French horn Trombone Euphonium Tuba Brass instruments produce sound through the vibration of the player's lips against a mouthpiece. The trombone commonly uses a slide. The trumpet is generally the highest standard orchestral brass instrument. The tuba is generally the lowest standard orchestral brass instrument. 3. STRINGS Examples: Violin Viola Cello Double bass The violin is generally the highest standard orchestral string instrument. The viola is larger and lower in pitch than the violin. The cello is commonly played while seated. The double bass is the lowest standard orchestral string instrument. Strings can produce sound by bowing or plucking. 4. PERCUSSION Examples: Timpani Snare drum Bass drum Cymbals Triangle Tambourine Xylophone Marimba Vibraphone Definite-pitch percussion instruments include the xylophone, marimba, vibraphone, and timpani. Many drums and cymbals produce sounds without a single definite pitch. Percussion instruments are not all unpitched. SECTION 5: VOCAL MUSIC 1. VOICE TYPES Soprano: Highest common adult female voice category. Mezzo-soprano: Middle adult female voice category. Alto or contralto: Lower adult female voice category. Tenor: Highest common adult male voice category. Baritone: Middle adult male voice category. Bass: Lowest common adult male voice category. SATB stands for soprano, alto, tenor, and bass. Voice ranges can overlap. A student's comfortable singing range is more important than a single note boundary. 2. VOCAL HEALTH Encourage proper posture and relaxed breathing. Use age-appropriate warm-ups. Encourage hydration. Avoid forcing students to sing outside a comfortable range. Recognize that adolescent voices change at different rates. Adapt vocal parts to accommodate changing voices. Range refers to the pitches a singer can produce. Tessitura refers to the range in which a singer is most comfortable and can sing sustainably. SECTION 6: AURAL SKILLS AND PERFORMANCE 1. LISTENING VOCABULARY Pitch: How high or low a sound is. Timbre: The quality or color of a sound. Dynamics: The loudness and softness of music. Articulation: How notes begin, connect, and end. Intonation: The accuracy of pitch. Balance: The relative loudness of musical parts. Blend: How well sounds combine within an ensemble. Tempo: The speed of the beat. Texture: The relationship among musical lines. Form: The overall organization of a musical composition. 2. CONDUCTING Conducting patterns communicate meter and coordinate performers. Two-beat pattern: Two main beats per measure. Three-beat pattern: Three main beats per measure. Four-beat pattern: Four main beats per measure. The preparatory gesture establishes the tempo and helps cue the entrance. The ictus is the focal point of a beat gesture. A cutoff gesture indicates when performers should stop producing sound. Clear gestures, consistent tempo, and appropriate eye contact help ensembles perform together. 3. TUNING AND INTONATION Tuning establishes a reference pitch. Intonation describes the accuracy of performed pitches. A sharp pitch is too high. A flat pitch is too low. Musicians should listen to one another and adjust pitch, balance, and blend. Warm-ups prepare performers physically and musically. 4. TRANSPOSING INSTRUMENTS Some instruments sound at a different pitch from the written note. B-flat trumpet: Written C sounds as B-flat. B-flat clarinet: Written C sounds as B-flat. E-flat alto saxophone: Written C sounds as E-flat. F horn: Written C sounds as F. These are standard written-to-sounding relationships for the listed instruments. SECTION 7: MUSIC EDUCATION AND PEDAGOGY 1. IMPORTANT TEACHING APPROACHES Orff Schulwerk: Uses speech, movement, improvisation, and accessible percussion instruments. Kodály: Emphasizes singing, solfege, inner hearing, and sequential musicianship. Dalcroze Eurhythmics: Uses bodily movement to experience musical concepts, particularly rhythm. Suzuki: Emphasizes listening, repetition, early instrumental learning, and family involvement. Gordon Music Learning Theory: Emphasizes audiation, the ability to hear and comprehend music internally. 2. SOLFEGE Movable-do solfege connects syllables to scale degrees. Scale degree 1 = Do Scale degree 2 = Re Scale degree 3 = Mi Scale degree 4 = Fa Scale degree 5 = Sol Scale degree 6 = La Scale degree 7 = Ti Scale degree 8 = Do In movable do, Do represents the tonic of the key. In fixed do, syllables correspond to specific note names. Hearing is the physical process of perceiving sound. Audiation is the mental process of hearing and understanding music internally. 3. LESSON PLANNING A strong music lesson includes: A clear learning objective. Activities that directly support the objective. Opportunities for guided and independent practice. Assessment to determine whether students met the objective. Feedback and adjustments for future instruction. Example objective: Students will accurately clap and identify quarter notes, eighth notes, and quarter rests in a four-beat measure. An appropriate assessment would ask students to perform or identify the rhythms. 4. TYPES OF ASSESSMENT Diagnostic assessment: Identifies what students know before instruction. Formative assessment: Checks learning during instruction and helps the teacher adjust instruction. Summative assessment: Evaluates learning after a unit or at the end of instruction. Performance assessment: Requires students to demonstrate a skill, such as singing a melody. Rubric: Defines assessment criteria and performance levels. Example: If students struggle with a rhythmic pattern during rehearsal, the teacher can model the rhythm, provide guided practice, and reassess their performance. 5. CLASSROOM MANAGEMENT AND INCLUSION Give clear instructions. Model the expected behavior. Establish routines for distributing instruments and transitioning between activities. Choose age-appropriate repertoire. Use suitable vocal and instrumental ranges. Adapt instruction to accommodate different abilities and learning needs. Provide multiple ways for students to demonstrate understanding. Give specific and constructive feedback. Maintain a respectful environment where students can participate without humiliation. 6. COPYRIGHT AND PROFESSIONAL ETHICS Copyright generally protects original musical works and sound recordings, subject to applicable law. Purchasing sheet music does not automatically grant permission to copy or distribute it. Educational use is not automatically exempt from copyright restrictions. Fair use depends on the circumstances and relevant legal factors. Use appropriately licensed scores, recordings, and digital resources. Respect student privacy and follow school policies when recording or sharing performances. Credit sources and respect creators' rights. SECTION 8: MUSIC TECHNOLOGY AND ACOUSTICS MIDI: Represents musical performance data, including notes, timing, and velocity. MIDI is not itself a recorded audio waveform. Digital audio: Represents sound using digital samples. Microphone: Converts sound into an electrical signal. Amplification: Increases the level of an audio signal. Reverberation: The persistence of sound due to reflections in an environment. Acoustics: The study of how sound behaves in spaces and environments. Frequency: Measured in hertz and related to perceived pitch. Amplitude: Describes the magnitude of a sound wave or signal and is related to perceived loudness. Timbre: The quality that helps distinguish sounds produced by different instruments or voices. Important distinction: Frequency is not the same as loudness. Higher frequency generally corresponds to higher pitch, while greater amplitude generally corresponds to greater loudness. SECTION 9: MUSICAL NOTATION AND TERMS 1. STAFF AND CLEFS Treble clef lines, from bottom to top: E, G, B, D, F. Treble clef spaces, from bottom to top: F, A, C, E. Bass clef lines, from bottom to top: G, B, D, F, A. Bass clef spaces, from bottom to top: A, C, E, G. Ledger lines extend the staff to represent pitches above or below it. 2. ADDITIONAL TERMS Enharmonic notes: Notes that sound the same in equal temperament but have different spellings, such as C-sharp and D-flat. Anacrusis: An incomplete opening measure, also called a pickup. Ostinato: A repeated musical pattern. Rubato: Expressive flexibility in tempo. Cadenza: A soloistic passage, often virtuosic, traditionally associated with a concerto. A cappella: Singing without instrumental accompaniment. Counterpoint: The combination of independent melodic lines. Basso continuo: A Baroque accompaniment practice involving a bass line and harmonic realization.
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