Cell death assay with fluorescent dyes

assay techniques

Biochemistry Laboratory Practical: Enzyme Reactions and Assays

antagonists in functional assays

Assays and Techniques

Fire Assay and Metallurgical Analysis

Blood sampling and assays

Serology Assays

Lesson 10. Assay of Ascorbic Acid Tablets

Question 1: Application of Methylation Detection Methodologies A researcher wants to monitor the early emergence of platinum resistance in a new cohort of HGSOC patients using liquid biopsies. They must choose between High-Resolution Melt (HRM) analysis and Sanger sequencing of bisulfite-converted cell-free DNA (cfDNA). Task: Contrast these two methodologies and explain which approach should be used first to screen for general methylation changes in the NKAPL promoter across the entire cohort, and why a second, independent technique is then required. To monitor the emergence of platinum resistance in a cohort of HGSOC patients using liquid biopsies, the methodologies of High-Resolution Melt (HRM) analysis and Sanger sequencing can be contrasted and applied as follows: Comparison of Methodologies • High-Resolution Melt (HRM) Analysis: This is a post-PCR technique that measures the overall methylation status of a targeted genomic region (amplicon). It relies on the fact that methylated and unmethylated DNA, after bisulfite conversion, will have different GC contents, causing them to melt at different temperatures. It is considered a low-cost, efficient screening tool but does not provide details on specific CpG sites. • Sanger Sequencing (of bisulfite-converted DNA): This technique provides site-specific resolution, allowing the researcher to determine the exact methylation percentage at every individual CpG site within the region of interest. However, it is more technically demanding because bisulfite conversion can degrade DNA, making it "really really difficult to sequence". Screening Strategy HRM analysis should be used first to screen the entire cohort for general methylation changes in the NKAPL promoter. The rationale for this sequence is that HRM acts as an effective initial filter to identify samples where "something potentially interesting" is occurring regarding DNA methylation patterns. Because liquid biopsies (cfDNA) contain only a small fraction of circulating tumor DNA (ctDNA) amidst a high background of non-tumor DNA, a broad, efficient screening tool like HRM is practical for processing many samples at multiple time points. Requirement for a Second, Independent Technique A second, independent technique like Sanger sequencing is required to confirm the initial findings and provide functional insight. 1. Validation: It is standard practice to confirm results from one epigenetic assay with an independent method to ensure accuracy and rule out artifacts. 2. Site-Specific Resolution: While HRM indicates a general change in the region, sequencing reveals which specific CpG sites within the NKAPL promoter are being modified. This detail is crucial for subsequent mechanistic studies, such as using CRISPR-dCas9 systems to edit those specific sites to prove they directly modulate chemoresistance.

FA All ASSAYS

Lecture 4: Antagonists in Functional Assays

Epigenomic Assays for Dissecting Non-Coding Function

Lesson 4.4 Total Flavonoid Content and Antioxidant Assay

Experiment 4. Acidimetric Assay by Double-Indicator Titration

Experiment 6. Assay of Ammonium Chloride

Comprehensive Macromolecules, Enzyme Assays, and Cell Transport in Cow Tissues

L2: M/o in medical / pharma assays

Module 20 - Plaque Assay of Phage

7 - Drug binding: binding assays