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Light Microscope
Uses visible light for illumination.
Electron Microscope
Uses a beam of electrons for illumination.
Magnification of Light Microscope
Up to ~1,000x.
Magnification of Electron Microscope
Up to ~2,000,000x.
Resolution of Light Microscope
~0.25-0.3 µm.
Resolution of Electron Microscope
~0.001 µm.
Specimen Type for Light Microscope
Can observe live or dead specimens.
Specimen Type for Electron Microscope
Can only observe dead or dried specimens.
Image Output of Light Microscope
Produces colored images.
Image Output of Electron Microscope
Produces black and white images (can be colorized digitally).
Lens Material of Light Microscope
Made of glass.
Lens Material of Electron Microscope
Uses electromagnetic lenses.
View Format of Light Microscope
2D (some stereo microscopes offer 3D).
View Format of Electron Microscope
2D or 3D depending on type (TEM or SEM).
Preparation Time for Light Microscope
Minutes to hours.
Preparation Time for Electron Microscope
Several days.
Portability of Light Microscope
Compact and movable.
Portability of Electron Microscope
Large, stationary equipment.
Cost of Light Microscope
Affordable for schools and hobbyists.
Cost of Electron Microscope
Expensive; used in advanced labs.
Applications of Light Microscope
Used in education, basic biology, and live cell study.
Applications of Electron Microscope
Used in nanotech, virology, and materials science.
Field of View (FOV)
The diameter of the visible area under the microscope.
Relationship between Magnification and FOV
As magnification increases, the field of view decreases.
Cell Theory - First Tenet
All living organisms are composed of one or more cells.
Cell Theory - Second Tenet
The cell is the basic unit of structure and function in living things.
Cell Theory - Third Tenet
All cells arise from pre-existing cells.
Robert Hooke
Coined the term 'cell' after observing cork under a microscope.
Matthias Schleiden
Proposed that all plants are made of cells.
Theodor Schwann
Concluded that all living things are composed of cells.
Rudolf Virchow
Added the tenet that all cells arise from pre-existing cells.
Conversion of Millimetre to Micrometre
1 mm = 1000 µm.
Example of Conversion
15 mm = 15 × 1000 µm = 15000 µm.
reliable data
data that has been judged to have a high level of reliability
can data be made more reliable
repetition and replication
validity
extent to which tests measure what was intended, how accurate the data inferences and data produced from tests are
biology
study of living things
organisms
living things that can respire to produce energy, grow, respond to stimuli, consume nutrients, reproduce and regulate their internal environment
biodiversity
range of different living things in a region
biosphere
any area on earth living things inhabit
genes
a section of DNA in a chromosome that codes for specific proteins
gene pool
sum of all genes including all different forms in a given population of one species
endemic
species native to region
biodiversity hotspot
an area with numerous endemic species a large number of endangered and threatened species
species
group of morphologically similar organisms that share a gene pool
ecosystem
all living organisms in a particular area and the non living components of their environments and their interactions
photosynthesis
chemical reaction using energy from the sun to convert CO2 + H2O to glucose and oxygen
population
a group of individuals belonging to the same species living in a particular area at the same time
global changes
climate, biochemical cycles, land use, species introduction
ecosystem function
provisioning services, cultural services, supporting services, regulating services
biological species concept
definition of species based on whether members can interbreed to produce fertile offspring
hybrid
non viable infertile offspring that can result from mating of two individuals from diff species
morphological species concept
definition of a species based physical characteristics (morphology)
phylogenetic species concept
a definition of species states a species compromises the smallest group of individuals sharing a common ancestor often determined through genetic analysis
common ancestor
a species from which to or more evolved from
phylogeny
the evolutionary ancestry of a species
extent
living
phylogenetic tree
based on common ancestry often determined through genetic analysis shows evolutionary relationship between organisms
how are all components of an ecosystem linked
cycling of nutrients
genetic diversity
variety of genetic traits within species
spatial
space occupied
spacial patterns are used to
understand extent and distribution of individuals in a species
distribution
location pattern of members of a species in an area
random distribution
irregular (cacti in desert)
clumped distribution
individuals in diff sized groups that are spaced out in non uniform manner (meerkats)
uniform distribution
evenly spaced individuals (trees in forest competing for sun)
bacteria
microscopic unicellular organisms that are prokaryotic
temporal
time, provides details about biodiversity in a certain area over a certain time period
taxa
group of organisms
what are the taxa
domain, kingdom, phylum, class, order, family, genus, species
characteristics for classifying
phylogeny, morphological/ structure, molecular data/ DNA and proteins
methods of reproduction
asexual and sexual
organelle
specialised structure or component within a cell that has a specific function
what are proteins made of
sequences of amino acids
amino acids
nitrogen containing compound
dorsal nerve chord
bundle of nerve fibres that run down the back
what are animals
multicellular heterotrophic eukaryotes that are classes based on body plans and developmental pathways
embryo
early stage in development
discussion
explain what results mean
autotrophic
produce own simple sugars through photosynthesis (plants)
what separates the to groups of plants
have or don't have vascular tissue
vascular tissue
tissue devoted to bulk transport of water, nutrient, sugars and other substances
non vascular plants
bryophytes (liverworts & mosses)
advantage of vascular tissue
transport more water faster therefore can grow faster
2 plant groups that reproduce using seeds
gymnosperms and angiosperms
seed
embryo with stored food supply
gymnosperms
non flowering plants have, pollen, naked seeds, seeds in cones
angiosperms
flowering plants that produce seeds in fruit
bryophytes
non vascular land plants
pteridophytes
seedless vascular plants, spores
classification seeds
tools used to identify organisms that belong to species that have already been discovered
how are organisms classified
physical features, habitats, ancestry
dichotomous
each step user has to choose between two options
evolution
slow incremental changes over time as organisms adapt to their environment
cladistics
a method of classifying organisms based on the characteristics they share
cladogram/phylogenetic tree
constructed using cladistics that show the evolutionary relationships between organisms
clade
A group of species that includes an ancestral species and all its descendants.
limitations of cladistics
when unrelated organisms have evolved similar characteristics independently
paraphyletic
group that doesn't contain all organisms descended from the most recent common ancestor
example of paraphyletic class
reptile because birds also descended from dinosaurs