Bradford assay
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6 Terms
What is the principle of the Bradford assay?
The Bradford assay measures protein concentration by dye binding to protein.
What dye is used in the Bradford method?
The dye used is Coomassie Brilliant Blue G-250.
What colour change occurs during the assay?
The dye changes from brown-red to blue when it binds protein.
At what wavelength is absorbance measured?
Absorbance is measured at 595 nm.
What protein properties affect dye binding?
Dye binding is affected by the protein’s amino acid composition, especially basic and aromatic residues.
Why is the Bradford assay considered sensitive?
he Bradford assay is sensitive because it detects low protein concentrations.
