Urine lab session

Method for urine SG

• Wipe any dust off the prism with tissue  

• Apply 2 drops of distilled water to the prism and look through the lens, pointing the prism at light  

• Ensure the line is set at 1.000 (USG)  

• If it is above or below adjust using a screwdriver  

• Wipe off distilled water with a clean tissue 

• Invert the urine sample and put one-two drops of urine onto the prism using a clean syringe/pipette  

• Look through and read the USG (Far right or can be middle)  

• Write down reading – is this within normal limits? 

• Rinse with water and dry, leaving ready for next user  

What are 3 urine concentrations

• Hypersthenuria (normal concentration) 

• Isothenuria 

• Hyposthenuria  

What is hypersthenuria for a cat

1.035-1.060

What is hypersthenuria for a dog

1.015-1.045

What is hypersthenuria for a rabbit

1.003-1.036

What is isothenuria

1.010-1.025

What is hyposthenuria

<1.010

Method for urine dipstick

• Put on gloves 

• Put dipstick flat onto absorbent tissue/inco pad   

• Using a pipette/syringe – apply one drop of urine to each reagent pad   

• OR dip the whole stick into the urine pot (for OSCE only)  

• Compare the colour of each reagent with the standards on the pack after required amount of time – this varies from 30s to 2m 

Method for urine sediment

• Invert sample to mix the urine  

• Using a pipette transfer 2-5ml of urine to an Eppendorf tube (conical tipped centrifuge tube) 

• Place the tube in the centrifuge ensuring it is balanced  

• Centrifuge for 5 mins at 2000rpm  

• Once stopped remove the tube and discard the supernatant by pipetting it off  

• Keep 0.5ml of supernatant in the tube  

• Add one drop of stain to the tube with a pipette 

• Swirl/flick the tube to resuspend the sediment in the supernatant  

• Use a pipette to transfer a drip on to a microscope slide  

• Place a coverslip over the sample  

• Look at under a x10 and x40 microscopic lens with a low light intensity