Reo/Rota- viruses

Orbibiruses

Rotoviruses

What two categories are w/in Reoviruses?

Segmented , non-eveloped dsRNA virus

What kind of virus is a Orbivirus?

They can combine to create a new virus and infect new species

What are benefits of being a segmented virus?

Culicoides - in early fall in the tropics

What is the vector to orbiviruses?

Partial uncoating of endolysozomes w/ early replication w/in the viral particle - in the cytoplasm

How are reoviruses going to evade the immune system?

Sheep

Deer (White tailed most affected)

Blue tongue is going to affect ___ more commonly and Enzootic hemorrhagic dz will affect ___ more commonly?

African horse sickness -in Africa and Thailand

Which Orbivirus will not be endemic to the U/S?

Bone marrow

Endotheilal cells - hemorrhage/ clotting issues

Orbiviruses will initially infect the ___ but will eventually affect the ____ cells?

High fever

Oronasla discharge

Hypersalivation

Oral necrosis (ulceration)

Lameness

Name clinical signs of Orbivirus?

African horse sickness

Pulmonary edema is most common with which orbivirus?

Enzootic hemorrhage dz

Sudden death is possible w/ which orbivirus?

Blue tongue virus

Abortion is possible w/ which orbivirus?

Edema of head/ neck

Ulceration in mouth

Pulmonary edema

Muscular hemorrhage

On necropsy what are we likely to see of an animal infected with/ orbivirus?

AGID/ELISA: Surviving

rtPCR: dead/ serotyping

AGID and ELISA is used to test for orbivirus in ____ animals. rtPCR is used to _____?

Palliative/ euthanasia

-dont do well after infected

-Commercial/ custom autogenous vax - not great for long term immunity only one strain

How is orbivirus tx?

Vesicular dz (FMD/ VSV)

MCF

Adenovirus

What differentials should you distinguish Orbivirus from?

Species - can cause diarrhea in neonates

Rotaviruses are typically ___ specific?

Feco-oral

Infects villi tips/ mature enterocytes

How is rotavirus transmitted and where does it infect?

Non-bloody (unless secondary bacterial infection)

Watery D

What are clinical signs of Rotavirus?

Parvovirus - 5-7 days

Rotavirus - 3-4 days - only affects villus tips

What lasts longer parvovirus or rotavirus?

Electron microscopy - not often

ELISA/ Latex agglutination

FA

How is rotavirus dx?

Oral vax

Colostrum/ cleaning

How is Rotavirus prevented?

Supportive care

Manage concurent infections

How is Rotavirus tx?

Parvo

Coronavirus

BVDV

Parasites

FB

Name a few differentials to R/O if you suspect rotavirus?

A dsRNA virus - nonenveloped

What kind of virus is birnavirus?

Transcription w/in viron in the cytoplasm

budding

How will birnavirus evade the immune system?

Ecchymotic hemorrhages on pectoral muscles

Enlarged Bursa of Fabricius

Depend on age and strain

What are clinical signs of Birnaviruses?

Fecal oral transmission to GI tissue -> liver kipper cells -> B-cells

What is the target of infectious burial dz virus?

Immunosuppression

What are lingering symptoms of infectious burial dz virus?

ELISA

Viral Isolation on eggs

rt-PCR

Genome sequencing

What are methods of Dx infectious burial dz virus?

Depop/repop

All-in/All-out

Vax

Biosecurity

How is infectious burial dz virus tx/ prevented?