SEMEN - LEC

SPERMATOZOA

5%

SEMINAL FLUID

60%-70%

PROSTATE FLUID

20-30%

BULBOURETHRAL GLANDS

5%

Testis/Epididymis

0.15ml (5%)

Average approximately spermatozoa 80 million/ml

Seminal Vesicle

1.5-2ml (50-65%)

Fructose (1.5-6.5 mg/ml)

phosphorylcholine ergothioneine, ascorbic acid, flavins prostaglandins,

bicarbonate

Prostate

0.6-0.9ml (20-30%)

Prostate, spermine, citric acid, cholesterol, phospholipids, Fibrinolysin, fibrinogenase, zinc acid, phosphatease, prostate-specific

Bulbourethral Glands

< 0.15ml (<5%)

Clear mucus

SEMINIFEFROUS TUBULES OF TESTES

SPERMATOGENESIS

EPIDIDYMIS

SPERM MATURATION

DUCTUS DEFERENS

PROPEL SPERM TO EJACULATORY DUCTS

SEMINAL VESICLES

PROVIDE NUTRIENTS FOR SPERM AND FLUID

PROSTATE GLAND

PROVIDE ENZYMES AND PROTEINS FOR COAGULATION AND LIQUEFACTION

BULBOURETHRAL GLANDS

ADD ALKALINE MUCUS TO NEUTRALIZE PROSTATIC ACID AND VAGINAL ACIDITY

DECREASED MOTILITY WITH NORMAL COUNT

VIABILTY

EOSIN-NIGROSIN STAIN

DECREASED MOTILITY WITH CLUMPING

MALE ANTISPERM ANTIBODIES

MIXED AGGLUTINATION REACTION AND IMMUNOBEAD TESTS

SPERM AGGLUTINATION WITH MALE SERUM

DECREASED COUNT

LACK OF SEMINAL VESICLE SUPPORT MEDIUM

FRUCTOSE LEVEL

NORMAL ANALYSIS WITH CONTINUED INFERTILITY

FEMAL ANTI-SPERM ANTIBODIES

SPERM AGGLUTINATION WITH FEMALE SERUM

HAMSTER EGG PENETRATION

SPERM ARE INCUBATED WITH SPECIESNONSCPECIFIC HAMSTER EGGS AND PENETRATION IS OBSERVED MICROSCOPICALLY

CERVICAL MUCUS PENETRATION

OBSERVATION OF SPERM PENETRATION ABILITY OF PARTNERES MIDCYCLE CERVICAL MUCUS

HYPO-OSMOTIC SWELLING

SPERM EXPOSED TO LOW SODIUM CONCENTRATIONS ARE EVALUATED FOR MEMBRANE INTEGRITY AND SPEM VIABILITY

IN VITRO ACROSOME REACTION

EVALUATION OF THE ACROSOME TO PRODUCE ENZYMES ESSENTIAL FOR OVUM PENETRATION