PHYBIO LAB - Protein Folding

4Y1S

Proteins are intricate biomolecules consisting of

long chains of amino acids

What structural classification of proteins represent the functional folded state, stabilized by molecular forces

Tertiary Structure

Refers to the process of altering a protein’s state, temporarily or permanently, by disrupting molecular forces

Denaturation

Denaturation is induced by factors like

pH, temperature, or presence of metal ions

Protein folding is thermodynamically driven, favoring states of

reduced free energy

The change in Gibbs free energy (∆G) is _-dependent

temperature

The change in Gibbs free energy (∆G) is comprised of

enthalpy (∆H) and entropy (∆S) components.

Protein folding can be significantly influenced by

the pH environment

The pH environment significantly influences protein folding by altering

ionization states

Bovine serum albumin (BSA) retains its native structure between pH 4 and 8, but outside this range, what happens

it adopts non-native conformations

Temperature can also affect the structure of protein folding by inducing

β-sheet formation during thermal denaturation and aggregation

Refers to the wavelength range where the absorbance of a compound significantly changes

Transition region