Proteomics Final

Primary Structure

Amino acid sequence

Bacisally just processed by DNA sequence

Proteomics methods: Mass spectrometry, Edman degradation, etc.

Rigid Peptide bond

Limited rotational freedom, almost always in trans configuration

Secondary Structure

Alpha helix and beta sheet.

Stabilized by hydrogen bonds between the backbone atoms

Tertiary Structure

3D shape of a protein

Defined by primary structure

Combination of motifs and domains

Single polypeptide chain

Quaternary Structure

Structure of two or more polypeptide chains

Homo- or hetero-oligomer

Large Complexes

Secondary Structure Prediction: Parametric Approach

Value for alpha helix and beta sheet likelihood assigned to each amino acid. Predicts strethches of 5+ amino acids.