Biotility Bace practice knowledge exam

cell theory states

All life forms are made from one or more cells. Cells only arise from pre-existing cells. The cell is the smallest form of life.

E.coli is an example of a eukaryote

false

Quality Assurance

ensure that all products meet standards of quality in manufacturing process

reasearch and development (R AND D)

identify candidate biopharmaceutical drugs

Manufacturing

make commercial quantities of biopharmaceutical drug

Which structure regulates the flow of molecules into and out of the cell?

Plasma Membrane

Which bacterial cell component is commonly isolated and used to digest DNA?

Restriction Enzyme

polymerase chain reaction ( PCR)

Heat-stable DNA polymerase

Digesting DNA

Restriction enzymes ( EcoRI)

Running DNA gels

Horizontal gel electrophoresis box

Running protein gels ( PAGE)

Vertical gel electrophoresis box

Joining pieces of DNA

DNA ligase

Spectrophotometer

Measuring protein or DNA concentration

A technician reapeated the maltase reaction described above, but the water bath failed to reach 37°C ( it is warmed to 30°C). What is the most likely out come?

The enzyme activity would decrease , yielding the same level of glucose over a longer time period.

Reaguarding the out come in question 8 , select the most appropriate action for the technician.

Report the problem to QA or individual in charge of repairs and place an out of service lable on the water bath

The byproduct of carbon dioxide benefits the brewing and baking industries. carbon dioxide is produced by microorganisms ,usually yeast, that obtain their energy by:

Anaerobic Respiration

Where is the energy in the triphosphate (ATP) ?

Phosphate bonds

In a DNA double helix, the two DNA chains are held together by:

Hydrogen bonds between the pair of bases

How do the DNA molecules in various species differ from one to another?

The number of genes and non-coding regions.

The nucleotide strands of DNA are oriented in opposite directions in a structure that is described as :

Anti-parallel

primary (1°) structure

Amino acids linked together by peptide bonds

Secondary (2°) structure

3D polypeptide with alpha-helices and beta-sheets due to hydrogen bonding.

Tertiary (3°) structure

Final 3D protein shape

Quaternary (4°) structure

Multiple polypeptides forming a larger protein complex

Correct order of assembly in the cell:

amino acid, polypeptide, protein

Why does increasing the temperature of the reactents increase the rate of chemical reaction?

Heat energy causes the particales of the reactents to move faster, and therefore react with each other more frequently

Why does the rate of chemical reaction increase with increasing temperature ?

Increase temperature increase the frequency of collisions between molecules

Why are enzyme solutions always prepare using a buffered solvent

cell theory states

All life forms are made from one or more cells. Cells only arise from pre-existing cells. The cell is the smallest form of life.

E.coli is an example of a eukaryote

false

Quality Assurance

ensure that all products meet standards of quality in manufacturing process

reasearch and development (R AND D)

identify candidate biopharmaceutical drugs

Manufacturing

make commercial quantities of biopharmaceutical drug

Which structure regulates the flow of molecules into and out of the cell?

Plasma Membrane

Which bacterial cell component is commonly isolated and used to digest DNA?

Restriction Enzyme

polymerase chain reaction ( PCR)

Heat-stable DNA polymerase

Digesting DNA

Restriction enzymes ( EcoRI)

Running DNA gels

Horizontal gel electrophoresis box

Running protein gels ( PAGE)

Vertical gel electrophoresis box

Joining pieces of DNA

DNA ligase

Spectrophotometer

Measuring protein or DNA concentration

A technician reapeated the maltase reaction described above, but the water bath failed to reach 37°C ( it is warmed to 30°C). What is the most likely out come?

The enzyme activity would decrease , yielding the same level of glucose over a longer time period.

Reaguarding the out come in question 8 , select the most appropriate action for the technician.

Report the problem to QA or individual in charge of repairs and place an out of service lable on the water bath

The byproduct of carbon dioxide benefits the brewing and baking industries. carbon dioxide is produced by microorganisms ,usually yeast, that obtain their energy by:

Anaerobic Respiration

Where is the energy in the triphosphate (ATP) ?

Phosphate bonds

In a DNA double helix, the two DNA chains are held together by:

Hydrogen bonds between the pair of bases

How do the DNA molecules in various species differ from one to another?

The number of genes and non-coding regions.

The nucleotide strands of DNA are oriented in opposite directions in a structure that is described as :

Anti-parallel

primary (1°) structure

Amino acids linked together by peptide bonds

Secondary (2°) structure

3D polypeptide with alpha-helices and beta-sheets due to hydrogen bonding.

Tertiary (3°) structure

Final 3D protein shape

Quaternary (4°) structure

Multiple polypeptides forming a larger protein complex

Correct order of assembly in the cell:

amino acid, polypeptide, protein

Why does increasing the temperature of the reactents increase the rate of chemical reaction?

Heat energy causes the particales of the reactents to move faster, and therefore react with each other more frequently

Why does the rate of chemical reaction increase with increasing temperature ?

Increase temperature increase the frequency of collisions between molecules

Why are enzyme solutions always prepare using a buffered solvent at a specific pH?

A buffered solvent is a resistant to fluctuations in pH, because it is made with a combination of a weak acid and it's made with a combination of a weak acid and it's conjugate base. Enzymes lose function outside of a narrow pH range.

Naturally occurring enzymes invovled with DNA replication, and which are also used as tools in biotechnology include :

DNA polymerase , DNA ligase

If a structural Gene's code is "TAC ATG CCC TTA ATC", What will the mRNA transcript be?

AUG UAC GGG AAU UAG

During the process of translation:

mRNA is translated into proteins

Where does translation take place in prokaryotes?

At the ribosomes

Humans have two copies of each cromosome, and therefore are:

Diploid

The observable traits of an organism are referred to as the:

Phenotype

The type of cell division in which haploid gametes are produced is called

Meiosis

What is the chance a child will be born with cystic fibrosis ( a homozygous recessive trait (aa)) if both the mom and dad are heterozygous (Aa) ?

25% chance of having the disorder

If DNA segments changes from GGTTAG to GGATAG this is a:

Nucleotide base substitution

monocolonal atibodies for scientific research can be produced in human cells

True

How many grams is 2.6kg?

2600

Plasmid DNA is purified using an ion exchange column chromatography. why?

Because DNA is negatively charged, binds to the positively charged resin, and is eluted with increasing salt.