Chem lab final

studied byStudied by 0 people
0.0(0)
learn
LearnA personalized and smart learning plan
exam
Practice TestTake a test on your terms and definitions
spaced repetition
Spaced RepetitionScientifically backed study method
heart puzzle
Matching GameHow quick can you match all your cards?
flashcards
FlashcardsStudy terms and definitions

1 / 35

flashcard set

Earn XP

Description and Tags

Chemistry

36 Terms

1

Buffer

A solution that resists changes in pH upon the addition of small amounts of acid or base.

New cards
2

ka

ka=(H+)(A-)/(HA)

New cards
3

pKa

The negative logarithm of the acid dissociation constant (Ka), indicating the strength of an acid.

New cards
4

Henderson-Hasselbalch equation

An equation used to calculate the pH of a buffer solution, given by pH = pKa + log([A-]/[HA]).

New cards
5

Beer lambert equation

A=€cl

New cards
6

stronger acid = ___ pka

smaller

New cards
7

Lysozyme

An enzyme that catalyzes the hydrolysis of glycosidic bonds in peptidoglycan, leading to cell wall rupture.

New cards
8

Peptidoglycan

A polymer consisting of sugars and amino acids that makes up the cell wall in bacteria.

New cards
9

lysozyme

cataylzes the hydroylsis of Beta 1 to 4 glycosidic bonds between NAG and NAM

New cards
10

Ion-Exchange Chromatography

A technique used to separate molecules based on their net charge.

New cards
11

Homodimer

A protein composed of two identical subunits.

New cards
12

Cation exchange

A method using negatively charged resin to bind positively charged proteins. (CM sepharose) -positive charged will bind

New cards
13

Anion exchange

A method using positively charged resin to bind negatively charged proteins. (DEAE sepharose)- negative charge will bind

New cards
14

pH < pI

protein has positive charge

New cards
15

pH > pI

protein has negative charge

New cards
16

lysozyme PI

very high -10.7

New cards
17

Biuret method

A colorimetric assay for detecting the presence of proteins based on the formation of a colored complex with copper II ions.
Color changes from light blue to dark purple

not very sensitive

New cards
18

ELISA

A technique that uses antibodies to detect the presence of specific antigens in a sample.

New cards
19

function of BSA in ELIZA

excess inert protein that locks sites not covered by protein

New cards
20

Michaelis-Menten equation and plot

knowt flashcard image

New cards
21

lineweaver burke plot

knowt flashcard image

New cards
22

Salt precipitation

A method used to purify proteins by adding salt to precipitate proteins out of solution. disrupt hydration sphere, salt pulls water molecules from surface of protein, forcing protein molecule to aggregate

-maintains protein native structure

New cards
23

how is got activity indirectly measured

  • oxaloacetate reacts with NADH to create L-malate and

  • NAD+, NADH absorbs at 340 but NAD+ does not

New cards
24

Heat denaturation

The process by which proteins lose their structural integrity and function due to high temperature. irreversible

New cards
25

IU/Ml equation:
Specific Activity equation:

Total GOT activity equation:

%yield fraction:

Degree of purity:

knowt flashcard image

New cards
26

Coomassie Blue

A staining dye used in electrophoresis. we use with acid/methanol mixture to fix proteins to the gel to prevent protein form diffusing in gel or being washed out

New cards
27

BME (Beta-Mercaptoethanol)

A reducing agent used to break disulfide bonds in proteins during SDS-PAGE.

New cards
28

SDS-PAGE

A technique that separates proteins based on their size by using polyacrylamide gel electrophoresis with sodium dodecyl sulfate.

New cards
29

Native PAGE

Polyacrylamide gel electrophoresis that maintains the native structure and charge density of proteins.

New cards
30

Polyacrylamide gel

A cross-linked polymer of acrylamide and bis-acrylamide used as a medium for gel electrophoresis to separate proteins based on size

New cards
31

higher % acrylamide to seperate ___ (big/small) proteins

small- dense matric- restrict movement of the large proteins

New cards
32

SDS

used to break non-covalent interactions (all become neg charged and linear)

New cards
33

BME

  • reducing agent to reduce (break) disulfide bonds

New cards
34

reducing conditions

  • BME+SDS

  • disulfide bonds broken

  • non-cov broken

New cards
35

non-reducing conditions

  • SDS only

  • disulfide not broken

  • non- cov broken

New cards
36

bromophenol blue in sds-page

doesn’t stain proteins, just shows progress of electrophoresis

New cards

Explore top notes

note Note
studied byStudied by 18 people
60 days ago
5.0(1)
note Note
studied byStudied by 125 people
706 days ago
4.5(4)
note Note
studied byStudied by 15 people
789 days ago
5.0(1)
note Note
studied byStudied by 2 people
67 days ago
5.0(1)
note Note
studied byStudied by 40 people
941 days ago
5.0(3)
note Note
studied byStudied by 12 people
661 days ago
5.0(1)
note Note
studied byStudied by 29 people
963 days ago
5.0(1)
note Note
studied byStudied by 505 people
1 hour ago
4.8(5)

Explore top flashcards

flashcards Flashcard (34)
studied byStudied by 10 people
148 days ago
5.0(1)
flashcards Flashcard (80)
studied byStudied by 5 people
483 days ago
5.0(1)
flashcards Flashcard (124)
studied byStudied by 35 people
427 days ago
5.0(1)
flashcards Flashcard (20)
studied byStudied by 8 people
396 days ago
5.0(1)
flashcards Flashcard (59)
studied byStudied by 11 people
774 days ago
5.0(1)
flashcards Flashcard (37)
studied byStudied by 4 people
767 days ago
5.0(1)
flashcards Flashcard (59)
studied byStudied by 4 people
233 days ago
5.0(1)
flashcards Flashcard (94)
studied byStudied by 85 people
14 days ago
5.0(1)
robot