Nutrient Testing

SBI4U

Benedict Test

Benedict Test

• Test for: Monosaccharides (carbohydrates)

• Mix with sample, heat over hot water 5 minutes

• Blue → Orange

• Note: Does NOT test for disaccharides

Lugol Test

• Test for: Starch (a polysaccharide)

• Uses: Iodine

• Mix with sample

• Turns blue

Biuret Test

• Test for: Proteins

• Uses: Potassium hydroxide (KOH)

• Mix with sample

• Turns purple

Sudan Red Test

• Test for: Lipids

• Uses: Fat-soluble dye

• Mix with sample

• Concentrated spots of colour