Identify which type of microscopy was used to obtain a given picture

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Biology

Cells

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16 Terms

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Phase contrast microscopy (light)

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Fluorescence microscopy

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transmission electron microscope (TEM)

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Scanning electron microscope (SEM)

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Brightfield/Light/Compound Microscope

Type: Optical microscope

Pros: Inexpensive, can view living organisms

Cons: Low resolution, medium magnification, samples must be stained

<p>Pros: Inexpensive, can view living organisms</p><p>Cons: Low resolution, medium magnification, samples must be stained</p>
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Fluorescence Microscope

Type: Optical microscope

Pros: Separate parts of a cell can be individually labeled

Cons: Fluorescence is temporary

<p>Pros: Separate parts of a cell can be individually labeled</p><p>Cons: Fluorescence is temporary</p>
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Phase Contrast Microscope

Type: Optical microscope

Pros: Can view living organisms, does not require staining, high contrast and resolution

Cons: Not good for thick samples, distorted "halo" around images

<p>Pros: Can view living organisms, does not require staining, high contrast and resolution</p><p>Cons: Not good for thick samples, distorted "halo" around images</p>
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Laser Scanning Confocal Microscope

Type: Optical microscope

Pros: controlled depth of field - good for thick samples, reduction of background noise

Cons: Medium magnification

<p>Pros: controlled depth of field - good for thick samples, reduction of background noise</p><p>Cons: Medium magnification</p>
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Transmission electron microscope (TEM)

Type: Electron microscope

Pros: Very high magnification, can view organelles and intercellular details

Cons: Preparation of samples requires fixation, expensive

<p>Pros: Very high magnification, can view organelles and intercellular details</p><p>Cons: Preparation of samples requires fixation, expensive</p>
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Scanning electron microscope (SEM)

Type: Electron microscope

Pros: 3D picture of samples, high magnification

Cons: Samples must be dried and covered in metal - difficult preparation, expensive

<p>Pros: 3D picture of samples, high magnification</p><p>Cons: Samples must be dried and covered in metal - difficult preparation, expensive</p>
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Brightfield Microscopy

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Phase Contrast Microscopy

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Differential Interference Contrast Microscopy

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Confocal Microscopy

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Transmission Electron Microscopy

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Scanning Electron Microscopy

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