Bio 006 - Biotech 6: Genomic DNA Extraction

Restriction Digest

Process of cutting DNA with enzymes.

Agarose

Gel used for electrophoresis to separate DNA.

Plasmid Mini-Prep

Extraction of plasmids from bacterial cells.

pARA-R

Red plasmid used as positive control.

Gel Electrophoresis

Technique to separate DNA fragments by size.

Buffer

Solution that maintains pH during reactions.

DNA Ladder

Molecular weight marker for gel electrophoresis.

Loading Dye

Colored solution to track DNA migration.

Minifuge

Small centrifuge for spinning samples.

Cheek Cells

Epithelial cells collected for DNA extraction.

Thermocycler

Device for controlled heating and cooling.

Centrifuge

Machine that separates components by spinning.

Supernatant

Liquid above solid residue after centrifugation.

Pellet

Solid residue formed after centrifugation.

Reagents

Substances used in chemical reactions.

Negative Control

Sample expected to show no effect.

Positive Control

Sample expected to show a known effect.

Agarose Powder

Solid form of agarose before mixing.

Gel Green

Stain used to visualize DNA in gels.

Water Bath

Device for maintaining samples at specific temperatures.

Vortex

Mixing technique using rapid spinning.

length

Gel Electrophoresis makes sure the DNA is the correct ___________