Reo/Rota- viruses

What two categories are w/in Reoviruses?

Orbibiruses

Rotoviruses

What kind of virus is a Orbivirus?

Segmented , non-eveloped dsRNA virus

What are benefits of being a segmented virus?

They can combine to create a new virus and infect new species

What is the vector to orbiviruses?

Culicoides - in early fall in the tropics

How are reoviruses going to evade the immune system?

Partial uncoating of endolysozomes w/ early replication w/in the viral particle - in the cytoplasm

Blue tongue is going to affect ___ more commonly and Enzootic hemorrhagic dz will affect ___ more commonly?

Sheep

Deer (White tailed most affected)

Which Orbivirus will not be endemic to the U/S?

African horse sickness -in Africa and Thailand

Orbiviruses will initially infect the ___ but will eventually affect the ____ cells?

Bone marrow

Endotheilal cells - hemorrhage/ clotting issues

Name clinical signs of Orbivirus?

High fever

Oronasla discharge

Hypersalivation

Oral necrosis (ulceration)

Lameness

Pulmonary edema is most common with which orbivirus?

African horse sickness

Sudden death is possible w/ which orbivirus?

Enzootic hemorrhage dz

Abortion is possible w/ which orbivirus?

Blue tongue virus

On necropsy what are we likely to see of an animal infected with/ orbivirus?

Edema of head/ neck

Ulceration in mouth

Pulmonary edema

Muscular hemorrhage

AGID and ELISA is used to test for orbivirus in ____ animals. rtPCR is used to _____?

AGID/ELISA: Surviving

rtPCR: dead/ serotyping

How is orbivirus tx?

Palliative/ euthanasia

-dont do well after infected

-Commercial/ custom autogenous vax - not great for long term immunity only one strain

What differentials should you distinguish Orbivirus from?

Vesicular dz (FMD/ VSV)

MCF

Adenovirus

Rotaviruses are typically ___ specific?

Species - can cause diarrhea in neonates

How is rotavirus transmitted and where does it infect?

Feco-oral

Infects villi tips/ mature enterocytes

What are clinical signs of Rotavirus?

Non-bloody (unless secondary bacterial infection)

Watery D

What lasts longer parvovirus or rotavirus?

Parvovirus - 5-7 days

Rotavirus - 3-4 days - only affects villus tips

How is rotavirus dx?

Electron microscopy - not often

ELISA/ Latex agglutination

FA

How is Rotavirus prevented?

Oral vax

Colostrum/ cleaning

How is Rotavirus tx?

Supportive care

Manage concurent infections

Name a few differentials to R/O if you suspect rotavirus?

Parvo

Coronavirus

BVDV

Parasites

FB

What kind of virus is birnavirus?

A dsRNA virus - nonenveloped

How will birnavirus evade the immune system?

Transcription w/in viron in the cytoplasm

budding

What are clinical signs of Birnaviruses?

Ecchymotic hemorrhages on pectoral muscles

Enlarged Bursa of Fabricius

Depend on age and strain

What is the target of infectious burial dz virus?

Fecal oral transmission to GI tissue -> liver kipper cells -> B-cells

What are lingering symptoms of infectious burial dz virus?

Immunosuppression

What are methods of Dx infectious burial dz virus?

ELISA

Viral Isolation on eggs

rt-PCR

Genome sequencing

How is infectious burial dz virus tx/ prevented?

Depop/repop

All-in/All-out

Vax

Biosecurity