MOLBIO LAB: RNA EXTRACTIONS

TRANSCRIPTION

copying of one strand of DNA into RNA by a process similar to that of DNA replication. This activity is catalyzed by RNA polymerase, occurs mostly in interphase.

PROMOTER

RNA polymerase and its supporting accessory proteins assemble on DNA at a specific site called______

INITIATION

RNA polymerase and its supporting accessory proteins assemble on DNA at a specific site called promoter

ELONGATION

RNA polymerases in both eukaryotes and prokaryotes synthesize RNA using the base sequence of one strand of the double helix as a guide.

TERMINATION

Rho factor, Hairpin loop (Prokaryotes)

ONE

There is at least _____tRNA for each amino acids

RIBOSOMES

Translation of information from nucleic acid to protein requires reading of the mRNA by _____

TRANFER RNA tRNA

Translation of information from nucleic acid to protein requires reading of the mRNA by ribosomes, suing adaptor molecules or

RIBOSOMAL RNA

The largest component of cellular RNA is _____, comprising 80% to 90% of the total cellular RNA.

rRNA

__is an important structural and functional part of the ribosomes, cellular organelles where proteins are synthesized.

snRNA

____RNA stays in the nucleus after its transcription by RNA polymerase I or III. These RNAs sediment in a range of 6 to 85

DIETHYL PYROCARBONATE (DEPC)

__can be added to water and buffers (Except for Tris buffer) to inactivate RNAses permanently.

GUANIDINIUM THIOCYANATE

Chaotrophic agent used to degrade proteins and lyse cells

PHENOL AND CHLOROFORM

added under acidic pH condition

ISOPROPANOL

RNA is recovered by precipitation with _____