HIBE PRACTICAL

atleast you will get a 1/50?

Black Stripe

LB Agar

Red Stripe

Ampicillin

Blue Stripe

Arabinose

Purple (pinkish to us) Stripe

Kanamycin

Green Stripe

Spectinomycin

Required PPE in lab

Goggles/tied-back hair/closed-toe shoes

what is this?

beaker

full form of dH20

distilled water

capacity of pipette pump?

25 ml

capacity of pipette pump?

10 ml

capacity of pipette pump?

2 ml

convert to nanometer: 1ml

1000 ul

convert to ml: 1 L

1000 ml

Independant Variable

what is manipulated or changed

Dependant Variable

thing which is affected by manipulation or change in independent variable

Positive Control

guaranteed to produce reaction from change in independent variables (so you know how the reaction actually should look like)

Negative Control

set to have no reaction; if it does, data is not valid.

you have to get 200 ul of a reagent; what do you use (micropipette, graduated cylinder, tabletop balance, analytical balance)

micropipette (P-1000)

\-use smallest measuring tool

function of analytical balance

measure the mass of an object with a high degree of accuracy and precision. (measure within 0.0001 grams.)

function of tabletop balance

A tabletop balance is a scientific instrument used to measure the mass of an object. (measure till 0.01 g)

Prepare 50 ml of LB agar with a concentration of 45g/L

45g/L→45g/1000ml=C

50ml=V

C\*V=M

45g/1000ml\*50ml=M

2\.25g=M

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