HIBE PRACTICAL

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21 Terms

1

Black Stripe

LB Agar

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2

Red Stripe

Ampicillin

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3

Blue Stripe

Arabinose

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4

Purple (pinkish to us) Stripe

Kanamycin

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5

Green Stripe

Spectinomycin

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6

Required PPE in lab

Goggles/tied-back hair/closed-toe shoes

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7
<p>what is this?</p>

what is this?

beaker

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8

full form of dH20

distilled water

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9
<p>capacity of pipette pump?</p>

capacity of pipette pump?

25 ml

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10
<p>capacity of pipette pump?</p>

capacity of pipette pump?

10 ml

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11
<p>capacity of pipette pump?</p>

capacity of pipette pump?

2 ml

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12

convert to nanometer: 1ml

1000 ul

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13

convert to ml: 1 L

1000 ml

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14

Independant Variable

what is manipulated or changed

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15

Dependant Variable

thing which is affected by manipulation or change in independent variable

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16

Positive Control

guaranteed to produce reaction from change in independent variables (so you know how the reaction actually should look like)

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17

Negative Control

set to have no reaction; if it does, data is not valid.

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18

you have to get 200 ul of a reagent; what do you use (micropipette, graduated cylinder, tabletop balance, analytical balance)

micropipette (P-1000)

-use smallest measuring tool

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19

function of analytical balance

measure the mass of an object with a high degree of accuracy and precision. (measure within 0.0001 grams.)

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20

function of tabletop balance

A tabletop balance is a scientific instrument used to measure the mass of an object. (measure till 0.01 g)

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21

Prepare 50 ml of LB agar with a concentration of 45g/L

45g/L→45g/1000ml=C

50ml=V

C*V=M

45g/1000ml*50ml=M

2.25g=M

<p>45g/L→45g/1000ml=C</p><p>50ml=V</p><p>C*V=M</p><p>45g/1000ml*50ml=M</p><p>2.25g=M</p><p></p><p></p>
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