BACTE RECALLS

4-cocci, bacilli, spirilla, pleamorphic

Number of bacterial morphologies and what are they?

Bright field microscope

In case dark field microscope is not available, which one can be a SUBSTITUTE?

flourescent microscope

In case dark field microscope is not available, which one can be used for leptospira ID?

10 minutes

Sodium hypochlorite is a useful disinfectant with an optimal contact time of_______

AOTA

Media used in isolating Bordatella spp:

-bordet gengou

-regan lowe

-Charcoal cephalexin blood agar (CCBA)

h influenza

Mousy odor of colonies is a characteristic of?

Psuedomonas aeruginosa

Tortilla like or grape like odor of colonies

M catarrhalis

Semen like or pungent smelling colonies

B pertussis

Fresh soil like smell of colonies

85% nitrogen

Campy gas includes

-85% nitrogen

-10% CO2

-5% O2

Bacterial vaginosis

Clue cells are a characteristic of?

G vaginalis

Causative agent of bacterial vaginosis with clue cells in the urine

Human blood bilayer Tween Agar

Media of choice for culturing G vaginalis

C albicans

One of the most probable causes of moniliasis

C trachomatis

Associated with non gonococcal urethritis infection

Mycoplasma

Smallest bacteria as big as a large virus

P aeruginosa

Bacteria having this colonial morphology: serrate, irregular edges

B anthracis

Bacteria having this colonial morphology: medusa head/iron head, box car shaped bacillus, bamboo rods, beaten egg consistency

B pertussis

Bacteria having this colonial morphology: mercury droplet

H influenza

Bacteria having this colonial morphology: tan, mucoid and convex

B bronchoseptica

Bordatella species positive to both urease and oxidase

P jirovecii

Most common pathogen causing pneumonitis in AIDS patients

M avium complex

Most common pathogen causing disseminated infections in AIDS patients

S bovis

Bacteria obtained from cows causing colon cancer

Haemophilus and Neisseria

CHOC is the medium of choice for_________

Poor growth, larger zone

If susceptibility agar is dry, what will happen?

Class II

Generally, BSC for viral culture is set at what class?

Class I

Generally BSC for personnel environment protection but no product protection is what class?

Class IV

Generally BSC for hemorrhagic fevers (Ebola, Lass, Machupo, Marburg, Congo-crimean) and C. Immitis is set at what class?

P aeruginosa

Most commonly isolated organisms in ICU

Chlamydospore

Structure of C albicans that grown on cornmeal agar

Germ tube

Structure of C albicans that grown on serum and is elongated

Psuedohyphae

Structure of C albicans that is elongated without dissociation (links of sausages)

Blastoconidia

Structure of C albicans that is usually seen in direct microscopic exam

Poliovirus

Acute flaccid paralysis agent

Enterovirus 71

Causative agent of HFMD

CJD agent

Which one is not sterilized?

-ascaris ova

-subtilis spores

-CJD agent

-mycobacteria

Prions

CHON 14-3-3; most resistant to disinfection

Cotton blue

The aman medium contains which stain?

Bacitracin

Which is not an antibiotic:

-bacitracin

-gentamycin

-erythromycin

-sulphonamide

Middlebrook 7H10

A clear mycobacterium medium for better colony distinction

Botolinum toxin

Most potent toxin

Tetanospasmin

blocks the release of glycine and GABA.

2nd most potent toxin

Diptheria toxin

3rd most potent toxin

C difficile

Agent of pseudomembraneous enterocolitis

C sordelii

Agent of sever TSS

C perfringens

Agent of gas gangrene

Viral nucleic acid

Hepatitis C test of choice "HCV-RNA"

S epidermidis

Non hemolytic, catalase positive and oxidase neg

Elementary body

Infectious unit of chlamydia

Virion

Complete viral particle

Helicobacter

Catalase and oxidase pos curved abacteria causing ulcers

Klebsiella

Mucoid colonies --++

Germ tube

C albicans in serum yields_________________

75-15 k

Count of bacteria after pasteurization-> 10-20% of bacteria shall remain

Capnocytophaga

Diagnosed by peripheral blood staining with giemsa or field stain

bromcresol purple

The pH indicator used to facilitate detection of decarboxylase activity

bromthymol blue

The pH indicator used in HE, TCBS, citrate

Neutral red

The pH indicator used in MAC, SSA

Phenol red

The pH indicator used in MSA and XLD

Saboraud's agar

Used for the gen cultivation and maintenance of fungal culture

Dengue

Transfusion transmissible infections except:

-dengue

-HCV

-HIV

-syphilis

IV (rapid growers)

M phlei and M smegmatis are members of Runyon group__________

HOH and CAMP

Can be used to identify group B strep

Bile solubility test

Used to ID S pnuemoniae (S)

PYR test

ID S pyogenes and enterococci

LAP test

ID all streptococci

Modified oxidase

Test to differentiate Micrococcus and Staphylococcus

Novobiocin resistance

Used to ID S saphrophyticus

Penicillin susceptibility

A wound specimen produced a medusa head colony and square end bamboo shaped appearance gram + rod. To further ID this organism. Which test should be performed next?

Sporothirix

Asteroid bodies are found in tissue of infected patients

NAT

Definitive diagnosis of HPV is made through___________

Coxsackie B

Virus assc with Myocarditis, percarditis

Adenovirus

Has a common group specific antigen located on the hexon protein

Human fibroblast cell

Human CMV replicates in vitro in_________________

Human embryonic cell

Varicella zoster virus replicates in vitro in_________________

Primary monkey kidney cells

Influenza A replicates in vitro in_________________

Varicella

Associated with Reye's syndrome

Urine

Recommended spx for JC virus isolation

Accept and process

Stool for adenovirus is received in the lab, what to do next?

HGV

What virus is found from male drug users that is known in US but not in the Philippines?

Adenovirus

Acute hemorrhagic cystitis in children and Acute respiratory distress syndrome is most likely associated with what virus

Kirby Bauer Method

What method is the most commonly used test for C/S today?

+-

DNAse reaction of S aureus and S epidermidis respectively

DNAse +

"SMS"

S aureus

Moraxella catarrhalis

Serratia marcescens

Serotyping

Capsule is for what testing/Grouping

Protein A

It is detected in Coagglutination testing

Virulence factor of Staph

Salmonella

Kauffman scheme

Group A strep

Most common pathogens in the throat

bile salts and brilliant green

Selective agent in S-S agar

Color change

Positive for beta lactamase chromogenic cephalosporin method

Methylene blue and resazurin

Markers of anaerobiosis

Calcium alginate

This swab must not be used because it is toxic to virus

Dacron

Best for virus spx collection

Cotton Swab

No to neisseria

>14 mm

Positive/sensitive to optochin must be at least?

-70

In what temp do viruses must be stored for transport in several days (>5 days)

70% alcohol

Disinfectant to be used in wound abscess collection

positive and negative

PYR reaction of S pyogenes and S bovis respectively

Virulence test

final diagnosis of diptheria is made from

(+) 45˚ precipitin formation

(-) no precipitin formation