L12 Using assays for screening
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14 Terms
The chromogenic assay (recap)
Screening by NMR
What is waterLOGSY
What does a waterLOGSY signal look like
Signal inversion occurs following binding to the protein. Aggregation can lead to inversion of the signal without the protein, in which case it is very difficult to tell if binding occurred or not.
Saturated Transfer Difference
STD signal example - what does the change in intensity of the peaks tell us
A larger decrease in peak size happens as a result of stronger binding interactions with the protein in the region of the proton signal
WaterLOGSY cocktails
Put in around 6 molecules at the same time and figure out which is which at the end
Pros and cons of WL and STD
What is SPR
SPR sensogram - what does it tell us
Pros and cons of SPR
Isothermal Titration Calorimetry
ITC results
Pros and cons of ITC
Note 
Flashcards (72)