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Explain the sequence of events that occur during ATP synthesis.
F0 component provides a channel for protons
protons transferred from a to c subunits
then proton is returned to mitochondrial matrix when c subunit faces a subunit
where is ATP generated?
3 beta subunits in T form converts ADP→ATP
t→O = atp release.
Explain uncoupling with regard to mitochondria.
Carry protons across inner mitochondrial membrane. NO atp synthesis occurs, proton gradient never forms.
Explain how the rate of oxidative phosphorylation is regulated.
Electrons dont flow unless ADP is available. Inhibition of electron transport chain and Acceptor control by energy charge.
What poisions affect oxidative phophorylation?
Cyanide is a severe blocker