The study of what genes are, how they carry info, how info is expressed, and how genes are replicated
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Define Gene
a segment of DNA that encodes a functional product, usually a protein
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Define Chromosome
Structure containing DNA that physically carries hereditary info: chromosomes contain the genes.
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Define Genome
All the genetic info in a cell
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Name the locations of genetic materials for:
* Eukaryotic cells * Prokaryotic cells
Eukaryote: Mitochondria, plasmids, nucleus
Prokaryote: Plasmids, chromosomes
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Vertical vs. Horizontal genetic flow
Vertical: parent → offspring
Horizontal: in the same population/generation
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Describe the steps of transcription
* initiation - RNA polymerase binds to the promoter and DNA unwinds * elongation - RNA is synthesized. DNA that has been transcribed rewinds. * termination - Transcription reaches the terminator. RNA and RNA polymerase are released. DNA helix re-forms
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What holds Okazaki fragments together?
DNA Ligase
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types of RNA:
* mRNA * tRNA * rRNA
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Where does Translation occur?
in ribsomes
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mRNA is translated in:
codons
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List the start and end codons
Start: AUG
End: UAA, UAG, UGA
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Redundancy of codons help with what?
Protect genetic code from harmful mutations
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What operon is usually off but can be triggered to turn on?
* A cell with pili will form a bridge with another cell to transfer DNA * direct
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Explain tranformation
* naked DNA (free donor DNA fragment) in the environment is taken into the cell. * indirect
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Explain transduction
* A bacteriophage takes up the donor’s cell DNA and when it enters another cell, it will incorporate the Donor bacteria DNA into the recipient’s DNA (lysogenic cycle) * indirect
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Generalized vs. Specialized Transduction
Generalized:
* The virus only has bacterial DNA in it. Does not give viral DNA to the recipient. * bacteriophages can pick up any portion of the host's genome.
\ Specialized:
* New, replicated virus has both viral and bacterial DNA. * bacteriophages pick up only specific portions of the host's DNA.
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F+ x F- cell
A cell with a pilus (F+) attaches to a cell without a pilus (F-). It transfer one strand of F plasmid over to the recipient. Now both are F+ and have pilus
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High-frequency transfer (HFr)
* plasmid jumps into the chromosome and becomes a part of it * when the chromosome duplicates, the plasmid and part of the chromosome is transmitted to a new cell (conjugation). * The plasmid/chromosome hybrid incorporated into the recipient chromosome
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How can antibiotic resistance spread?
High-frequency transfer
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Explain how Recombinant DNA technology works
* A gene (insulin) is integrated into a plasmid by Ligase. * Plasmid enters a cloning host cell (E.coli) through transformation. This cell lacks any extra plasmids that could complicate the gene expression of the plasmid. * Take and keep the cells that have the recombinant plasmid. * As cells multiply, the plasmid also replicates. Each cell contains the gene. (E. coli cells can now produce human insulin) * There can be billions within a few hours * Recombinant strain is maintained in culture for production purposes
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What is a PCR (Polymerase Chain Reaction)?
* amplifies the number of copies of DNA * rapidly increased the amount of DNA in a sample
\ Steps
1. Denature - breaks double helix into two strands 2. Anneal - a primer is added to each strand for DNA synthesis 3. Extension: the DNA strand is extended