Orientation to the Histopath Section of the Lab

- Clinical Chemistry

- Hematology

- Clinical Microscopy and Parasitology

- Microbiology

- Blood Bank

- Immunology/Serology

Divisions of Clinical Pathology

- Histopathology

- Morgue

- Cytology

- Cytogenetics

- Electron Microscopy

Divisions of Anatomical Pathology

Pathology

the study of diseases

Pathology

study of the structural and functional alterations in cells, tissues, and organs of the body, which lead to the formation of a disease process.

pathologist

a physician highly skilled in the identification and diagnosis of both normal and diseased tissues.

Anatomical Pathology

medical specialty that is concerned with the diagnosis of disease based on the gross, microscopic and molecular examination of tissues and organs.

Anatomical Pathology

further subdivided into sections, mainly of which include surgical pathology, histopathology, cytopathology and forensic pathology

surgical pathology

histopathology

cytopathology

diagnosis carried at the cellular level

forensic pathology

medico-legal cases, not limited to autopsy

anatomic pathologist

operates primarily on cells and tissues.

microscopic study and interpretation

In the hospital standard pathology laboratory,

the daily surgical and autopsy tissue is

processed for ___

pathologist

In the hospital standard pathology laboratory, the daily surgical and autopsy tissue is processed for

medical laboratory scientist or histologic technician

engaged with the preparation and staining of tissue sections from surgical or autopsy material for the reading of the pathologist.

BIOPSY

procedures from surgical operations

AUTOPSY

procedures post-mortem specimens

admittance or specimen (accession) number

Upon reaching the laboratory, the tissue is given an identifying ?

1. Fixation

2. Decalcification (for hard tissues only,

optional step)

3. Dehydration

4. Clearing

5. Impregnation (Infiltration)

6. Embedding

7. Trimming

8. Section Cutting

9. Staining

10. Mounting

11. Labeling

Order of Processing of tissues

Microscope

an optical instrument used to view specimens that are too small to be seen by the naked eye

Microtome

cutting machine especially designed to cut very thin sections of tissues (tissue ribbons)

Automatic Tissue Processor/Autotechnicon

machine that automatically fixes, dehydrates, clears and

infiltrates the tissues

Cryostat

for frozen sections

Staining Trough and Coplin Jar

being used to stain cells and tissues on glass microscope slides at laboratory testing.

Cytospin

a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined

post-mortem examination

Autopsy is also known as ?

AUTOPSY

a highly specialized surgical procedure that consists of a thorough examination of a corpse to determine the cause and manner of death and to evaluate any disease or injury that may be present.

AUTOPSY

primarily to determine the cause of death, the state of health of the person before he or she died

medical examiner or coroner.

They head and perform autopsies and investigate deaths.