Test For Macromolecules

Anatomy and Physiology 1 lab

What does Benedict's test for?

Reducing sugars (simple carbohydrates: mono- and disaccharides)

What is a negative indicator for Benedict's test?

Blue

What is a positive indicator for disaccharides in Benedict's test?

Green

What is a positive indicator for monosaccharides in Benedict's test?

Orange, yellow, or red

What does the IKI (Iodine Potassium Iodide) test for?

Starch only

What is a negative indicator for the IKI test?

Pale yellow to amber brown

What is a positive indicator for the IKI test?

Black, bluish black, or dark brownish black

What does the Biuret test for?

Proteins and amino acids

What is a negative indicator for the Biuret test?

Blue

What is a positive indicator for proteins and polypeptides in the Biuret test?

Light to dark purple

What is a positive indicator for amino acids in the Biuret test?

Pink

What does the Evaporation test for?

Lipids

What is a negative result for the Evaporation test?

Aqueous solution evaporates

What is a positive result for the Evaporation test?

Lipids do not evaporate and leave an oily ring on brown paper

What does the Tween test for?

Lipids

What is a negative result for the Tween test?

No layers or droplet formation (no bubbles)

What is a positive result for the Tween test?

Lipids separate into smaller droplets (bubbles) or form a layer on water