Citric Acid Cycle

Citrate synthase

Enzyme in step 1

aconitase

Enzyme in step 2

Isocitrate dehydrogenase

Enzyme in step 3

alpha-ketpglutarate dehydrogenase complex

Enzyme in step 4

succinyl-CoA synthetase

Enzyme in step 5

Succinate dehydrogenase

Enzyme in step 6

Fumarase

Enzyme in step 7

Malate dehydrogenase

Enzyme in step 8

Acetyl-CoA

Input in CAC

Citrate

Step 1: Acetyl-CoA + Oxaloacetate -----> ?

Isocitrate

Step 2: Citrate -----> ?

alpha-ketoglutarate

Step 3: Isocitrate -----> ?

Succinyl-CoA

Step 4: alpha-ketoglutarate -----> ?

Succinate

Step 5: Succinyl-CoA -----> ?

Fumarate

Step 6: Succinate -----> ?

L-Malate

Step 7: Fumarate -----> ?

Oxaloacetate

Step 8: L-Malate -----> ?

Alpha-ketoglutarate dehydrogenase complex + malate dehydrogenase + isocitrate dehydrogenase

Which steps make NADH

Succinate dehydrogenase

Which steps make FADH2

Succinyl-CoA synthetase

What steps make GTP

3 NADH, 1 FADH2, 1 GTP

What does CAC make

Isocitrate dehydrogenase + alpha-ketoglutarate dehydrogenase complex

what steps release Co2

aconitase (moderate) + L-malate dehydrogenase

Energetic hurdles