BIOC Krebs Cycle Citric Acid Tricarboxylic Acid Cycle

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Step 1

citrate synthase
oxaloacetate + AcetylCoA + H2O —> citrate + CoASH

step 2

aconitase
citrate —> cis-aconitate +H2O (transition)
cis-aconitate + H2O —> isocitrate (using that water right back)

step 3

isocitrate dehydrogenase
isocitrate + NAD —> oxalosuccinate + NADH + H+ (transition)
oxalosuccinate —> alpha-ketoglutarate + CO2

FIRST CO2 RELEASE

step 4

alpha ketoglutarate dehydrogenase
alpha ketoglutarate + CoASH + NAD+ —> succinyl CoA + CO2 + NADH

SECOND CO2 RELEASE

irreversible

step 5

succinyl CoA synthetase

succinyl CoA + GDP + Pi —> succinate + GTP + CoASH

GTP GENERATING STEP

step 6

succinate dehydrogenase
succinate + FAD —> fumarate + FADH2

step 7

fumarase
fumarate + H2O —> L-malate

step 8

malate dehydrogenase
L-malate + NAD+ —> oxaloacetate + NADH + H+

citrate structure

5 carbon
cross shape @C3

cis-aconitate structure

6 carbon
right side COO^-

isocitrate structure

5 carbon

C3 has H-C-COO-

C4 has HO-C-H

oxalosuccinate structure

5 carbon

C3 has H-C-COO-
4C has C=O right side carbonyl

alpha-ketoglutarate structure

5 carbon
C=O at C4, right side carbonyl

succinyl CoA structure

4 carbon
S-CoA group

succinate structure

4 carbon
C2 + C3 are CH2

fumarate structure

4 carbon
C2 is CH

C3 is HC

L-malate structure

4 carbon
cross at C2

oxaloacetate structure

4 carbon
C2 has C=O right side carbonyl