GENBIO L1 Q3
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11 Terms
RECOMBINANT DNA
ARTIFICIALLY FORMED DNA CREATED BY COMBINING GENETIC MATERIAL FROM DIFFERENT SOURCES
ISOLATION OF DNA
DESIRED GENE AND VECTOR DNA ARE ISOLATED FROM THER RESPECTIVE ORGANISMS
CUTTING OF DNA
THE USE OF RESTRICTION ENZYMES THAT RECOGNIZE SPECIFIC DNA AND MAKE “STICKY ENDS”
INSERTION OF GENE INTO VECTOR
DESIRED GENE IS INSETED INTO THE PLASMID VECTOR USING DNA LIGASE
PLASMID VECTOR
THE TYPE OF VECTOR THE DESIRED GENE IS INSERTED INTO
DNA LIGASE
SEALS THE FRAGMENTS TOGETHER TO FORM RECOMBINANT DNA
TRANSFER INTO HOST CELL
THE RECOMBINANT PLASMID IS INTRODUCED INTO THE HOST CEORGANISM THROUGH TRANSFORMATION
TRANSFORMATION
THE PROCESS THAT INTRODUCES THE RECOMBINANT PLASMID INTO THE HOST ORGANISM
CLONING OF RECOMBINANT CELLS
PRODUCES MANY IDENTICAL COPIES OF THE DESIRED GENE
SCREENING AND SELECTION
RECOMBINANT AND NON-RECOMBINANT DNA ARE IDENTIFIED AND SEPERATED
EXPRESSION OF GENE
THE HOST ORGANISM USES THE DESIRED GENE TO PRODUCE THE DESIRED PROTEIN OR PRODUCT
Note 
Flashcards (20)