Lecture 9 Mitochondria pt 1

Select the true statement about the citric acid cycle

The major reactants in the citric acid cycle are CoA, NAD, GDP and FAD

Select the true statement about the citric acid cycle

The citric acid cycle is an aerobic process

Select the true statement about the citric acid cycle

The citric acid cycle occurs in the mitochondria of the cell

What are the major reactants in the citric acid cycle?

CoA, NAD, GDP, and FAD

The citric acid cycle is an aerobic process, this means this process requires

oxygen

Is the citric acid cycle aerobic or anaerobic

aerobic

Where does the citric acid cycle occur?

Mitochondria of the cell

In the mitochondria does ATP enter or leave?

ATP leaves the matrix to the cytoplasm for cellular use

In the mitochondria does H+ enter or leave the mitochondria?

H+ goes into the matrix through ATP synthase (passive flow down the electrochemical gradient , driving ATP synthase)

Where does Pi (phosphate) go in terms of the mitochondria? or does it enter at all?

Phosphate enters the matrix and is needed to make ATP

Which of the following about the mitochondrial electron-transport system is false?

All of these are true

True or false: Complex 4 donates electrons to O2 to form H2O

True

Proton-motive force is used to power ATP synthesis by:

Mitochondria, chloroplasts, and. bacteria

Which processes yield the most ATP? Citric acid cycle? Oxidation of pyruvate to acetyl-CoA? Glycolysis?

Tie between Citric acid Cycle and Glycolysis

Which processes yield the most ATP? When determining the ATP yield for each process, include ATP derived from reduced cofactors. Oxidation of pyruvate to acetyl-CoA? Glycolysis? Citric acid cycle?

Citric acid cycle

How are electrons moved from complex to complex?

By coupled oxidation-reduction reactions

“Hydrogen Ions cannot freely pass through the inner mitochondrial membrane”, true or false?

True

“ H+ concentration is higher in the inter membrane space that in the mitochondrial matrix” True or false?

True

“Oxidative phosphorylation relies on the H+ gradient generated by the electron transport chain”, True to False?

True

“The pH in the intermembrane space is higher than the pH in the mitochondrial matrix.” True or False?

False, the pH in the intermembrane space is lower (more acidic) than the pH in the mitochondrial matrix

“Energy is generated as a result of the difference in hydrogen ion concentration between the inter membrane space and the cytoplasm”, True or False?

False, energy is generated as a result of the difference in hydrogen concentration between the Intermembrane space and the mitochondrial matrix

“ATP synthase uses energy from ATP to move H+ into the mitochondrial matrix”, True or False?

False, ATP synthase uses the energy from the H+ gradient (proton motive force) to synthesize ATP from ADP and Pi, not the other way around

What is Ubiquinones main role?

To facilitate the movement of H+ out of the mitochondrial matrix

Is “Protons move down an electrochemical gradient through a proton channel at the interface between the a subunit and c subunit ring” part of the F0F1 ATP synthase binding mechanism for F1B subunit formation of the phosphoanhydride bond between ADP and Pi?

No this is not part of the F0F1 ATP synthase binding mechanism

Which of the following is NOT directly part of the F0F1 ATP synthase binding mechanism for F1B subunit formation of the phosphanhydride bond between ADP and Pi?

• The rotational position of the y subunit determines the conformational state of each B subunit

• All are part of the bidding mechanism

• Formation of the phosphoanhydride bond is catalyzed in the B subunit T state, and the ATP is released in the O state

• Each B subunit has three distinct conformational states: O, L and T.

• Protons move down an electrochemical gradient through a proton channel at the interface between the a subunit and c subunit ring.

• Protons move down an electrochemical gradient through a proton channel at the interface between the a subunit and c subunit ring.

What are the major reactants in the citric acid cycle?

acetylene CoA, NaD+, GDP and FAD

True or false, the citric acid cycle is an aerobic process?

True, (requires oxygen)

Where does the citric acid cycle occur?

In the mitochondria of the cell

What are the names of the central metabolic pathways?

Citric acid cycle, Kerbs Cycle, Tricarboxylic acid

Where is newly synthesized ATP located

in the mitochondrial matrix