Cell Fractionation

What is cell fractionation

Seperation of different parts of the cell

what is Homogenisation

Breaking the cell. Either by vibrating or blending cells into solution

why must the homogenised solution then by filtered

to remove complete cells or large fragments of cells

What is ultra centrifugation

the process of splitting up the organelles based on mass. The heavier the mass, the more centrifugal force

What are the conditions and explain them

Ice cold - to reduce enzyme activity so it doesnt break down organelles

Isotomic - so organelles dont burst or shrink due to loss or gain of water

Buffer - to maintain a constant PH so organelles don’t denture