Genetic Diversity

Spontaneous mutation

Errors madde by DNA polymerases during DNA replication

Genotype

Genetic makeup of an organism

Phenotype

An organism's physical appearance, or visible traits

Neopeptone agar

Rich, amino acids

YNB agar

Minimal nutrients, no amino acids

Rhodotorula glutinis wild type genotype

Genes for enzymes to make all 20 amino acids used to make proteins

Rhodotorula glutinis wild type neopeptone agar phenotype

Growth using amino acids in media

Rhodotorula glutinis wild type YNB agar phenotype

Growth by making amino acids from nutrients in media

Rhodotorula glutinis mutant genotype

Genes for enzymes to synthesize 19 of 20 amino acids to make proteins; due to gene mutation, cannot make enzyme to synthesize the amino acid lysine

Rhodotorula glutinis mutant neopeptone agar phenotype

Growth using amino acids in media

Rhodotorula glutinis mutant YNB agar phenotype

Death unless provided with amino acid

Missing Rhodotorula glutinis amino acid

Lysine